Combination of peptide nucleic acid beacon and nuclease S1 for clear-cut genotyping of single nucleotide polymorphisms

Analytical Biochemistry

Volumn 363, Issue 2, 2007, Pages 300-302

  Ye, Sheng ^a   Miyajima, Yoshitaka ^a   Ohnishi, Toshiyuki ^a   Yamamoto, Yoji ^a   Komiyama, Makoto ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CLEAR CUTS; GENOTYPING; NUCLEASE S1; PEPTIDE NUCLEIC ACID; SINGLE NUCLEOTIDE POLYMORPHISMS;

NUCLEIC ACIDS;

DNA; NUCLEASE; NUCLEIC ACID;

ANALYTIC METHOD; ARTICLE; ASPERGILLUS ORYZAE; CARBOXY TERMINAL SEQUENCE; DNA MICROARRAY; DNA SEQUENCE; GENOTYPE; MOLECULAR RECOGNITION; NUCLEIC ACID PROBE; PH MEASUREMENT; PHOTOCHEMICAL QUENCHING; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; SPECTROFLUOROMETRY;

EID: 33947268746     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.12.040     Document Type: Article

References (9)

1. Syvänen A.C. Accessing Genetic Variation: Genotyping Single Nucleotide Polymorphisms. Nat. Rev. Genet. 2 (2001) 930-942
2. Tyagi S., and Kramer F.R. Molecular Beacons: Probes that Fluoresce upon Hybridization. Nat. Biotechnol. 14 (1996) 303-308
3. Ortiz E., Estrada G., and Lizardi P.M. PNA molecular beacons for rapid detection of PCR amplicons. Mol. Cell. Probes 12 (1998) 219-226
4. Seitz O. Solid-Phase Synthesis of Doubly Labeled Peptide Nucleic Acids as Probes for the Real-Time Detection of Hybridization. Angew. Chem. Int. Ed. 39 (2000) 3249-3252
5. Kuhn H., Demidov V.V., Coull J.M., Fiandaca M.J., Gildea B.D., and Frank-Kamenetskii M.D. Hybridization of DNA and PNA Molecular Beacons to Single-Stranded and Double-stranded DNA targets. J. Am. Chem. Soc. 124 (2002) 1097-1103
6. Xi C., Balberg M., Boppart S.A., and Raskin L. Use of DNA and Peptide Nucleic Acid Molecular Beacons for Detection and Quantification of rRNA in Solution and in Whole Cells. Appl. Environ. Microbiol. 69 (2003) 5673-5678
7. Petersen K., Vogel U., Rockenbauer E., Nielsen K.V., Kølvraa S., Bolund L., and Nexø B. Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms. Mol. Cell. Probes 18 (2004) 117-122
8. Smolina I.V., Demidov V.V., Cantor C.R., and Broude N.E. Real-time monitoring of branched rolling-circle DNA amplification with peptide nucleic acid beacon. Anal. Biochem. 335 (2004) 326-329
9. Komiyama M., Ye S., Liang X., Yamamoto Y., Tomita T., Zhou J.-M., and Aburatani H. PNA for One-Base Differentiating Protection of DNA from Nuclease and Its Use for SNPs Detection. J. Am. Chem. Soc. 125 (2003) 3758-3762