Characterization of heparin-living bacteria interactions by chemiluminescence electrophoretic mobility shift assay

Analytical Biochemistry

Volumn 363, Issue 2, 2007, Pages 312-314

  Kang, Jonghoon ^a   Lee, Myung Soog ^a   Gorenstein, David G ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

ELECTROPHORETIC MOBILITY SHIFT ASSAY;

ELECTROPHORETIC MOBILITY;

HEPARIN; PHOSPHATE BUFFERED SALINE;

ARTICLE; BACTERIAL COUNT; BACTERIUM COLONY; CHEMOLUMINESCENCE; CONTROLLED STUDY; CROSS LINKING; DISSOCIATION CONSTANT; ELECTROBLOTTING; ESCHERICHIA COLI; GEL MOBILITY SHIFT ASSAY; MATHEMATICAL ANALYSIS; MICROBIAL ACTIVITY; NONHUMAN; OPTICAL DENSITY; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; ULTRAVIOLET RADIATION;

EID: 33947260110     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.01.038     Document Type: Article

References (19)

1. Berger R., Duncan M.R., and Berman B. Nonradioactive gel mobility shift assay using chemiluminescent detection. BioTechniques 15 (1993) 650-652
2. Ikeda S., and Oda T. Nonisotopic gel-mobility shift assay using chemiluminescent detection system. BioTechniques 14 (1993) 878-880
3. Patti J.M., Allen B.L., McGavin M.J., and Hook M. MSCRAMM-mediated adherence of microorganisms to host tissues. Annu. Rev. Microbiol. 48 (1994) 585-617
4. Rostand K.S., and Esko J.D. Microbial adherence to and invasion through proteoglycans. Infect. Immunol. 65 (1997) 1-8
5. Aoki K., Matsumoto S., Hirayama Y., Wada T., Ozeki Y., Niki M., Domenech P., Umemori K., Yamamoto S., Mineda A., Matsumoto M., and Kobayashi K. Extracellular mycobacterial DNA-binding protein 1 participates in mycobacterium-lung epithelial cell interaction through hyaluronic acid. J. Biol. Chem. 279 (2004) 39798-39806
6. Ascencio F., Fransson L.A., and Wadstrom T. Affinity of the gastric pathogen Helicobacter pylori for the N-sulphated glycosaminoglycan heparan sulphate. J. Med. Microbiol. 38 (1993) 240-244
7. Baron M.J., Bolduc G.R., Goldberg M.B., Auperin T.C., and Madoff L.C. Alpha C protein of group B Streptococcus binds host cell surface glycosaminoglycan and enters cells by an actin-dependent mechanism. J. Biol. Chem. 279 (2004) 24714-24723
8. Fleckenstein J.M., Holland J.T., and Hasty D.L. Interaction of an outer membrane protein of enterotoxigenic Escherichia coli with cell surface heparan sulfate proteoglycans. Infect. Immunol. 70 (2002) 1530-1537
9. Capila I., and Linhardt R.J. Heparin-protein interactions. Angew. Chem. Intl. Ed. Engl. 41 (2002) 391-412
10. Muñoz E.M., and Linhardt R.J. Heparin-binding domains in vascular biology. Arteriorsc. Thromb. Vasc. Biol. 24 (2004) 1549-1557
11. Bormann B.J., and Engelman D.M. Intramembrane helix-helix association in oligomerization and transmembrane signaling. Annu. Rev. Biophys. Biomol. Struct. 21 (1992) 223-242
12. Chen J.C., Zhang J.P., and Stephens R.S. Structural requirements of heparin binding to Chlamydia trachomatis. J. Biol. Chem. 271 (1996) 11134-11140
13. Dupres V., Menozzi F.D., Locht C., Clare B.H., Abbott N.L., Cuenot S., Bompard C., Raze D., and Dufrene Y.F. Nanoscale mapping and functional analysis of individual adhesions on living bacteria. Nat. Methods 2 (2005) 515-520
14. Garner M.M., and Revzin A. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: Application to components of the Escherichia coli lactose operon regulatory system. Nucleic Acids Res. 9 (1981) 3047-3060
15. Kang J., Lee M.S., and Gorenstein D.G. Quantitative analysis of chemiluminescence signals using a cooled charge-coupled device camera. Anal. Biochem. 345 (2005) 66-71
16. Kang J., Lee M.S., Watowich S.J., and Gorenstein D.G. Chemiluminescence-based electrophoretic mobility shift assay of RNA-protein interactions: Application to binding of viral capsid proteins to RNA. J. Virol. Methods 131 (2006) 155-159
17. Kang J., Lee M.S., and Gorenstein D.G. Chemiluminescence-based electrophoretic mobility shift assay of heparin-protein interactions. Anal. Biochem. 349 (2006) 156-158
18. J. Kang, Application of a cooled charge-coupled device (CCD) camera for the detection of chemiluminescence signal, Biologicals (in press), doi:10.1016/j.biologicals.2006.09.002.
19. Xu J., and Gordon J.I. Honor thy symbionts. Proc. Natl. Acad. Sci. USA 100 (2003) 10452-10459