Flow-FISH analysis and isolation of clostridial strains in an anaerobic semi-solid bio-hydrogen producing system by hydrogenase gene target

Applied Microbiology and Biotechnology

Volumn 74, Issue 5, 2007, Pages 1126-1134

  Jen, Chang Jui ^a   Chou, Chia Hung ^b   Hsu, Ping Chi ^b   Yu, Sian Jhong ^a   Chen, Wei En ^a   Lay, Jiunn Jyi ^b   Huang, Chieh Chen ^a   Wen, Fu Shyan ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b NATIONAL KAOHSIUNG UNIVERSITY OF APPLIED SCIENCES   (Taiwan)

Author keywords

Clostridium; Flow cytometry; Fluorescence in situ hybridization; Hydrogenase

Indexed keywords

ENZYMES; FISH; GENES; INDUSTRIAL WASTES; MICROBIOLOGY; STRAIN; YEAST;

CLOSTRIDIUM; FLUORESCENCE IN SITU HYBRIDIZATION; HYDROGENASE;

FLOW CYTOMETRY;

HYDROGEN; HYDROGENASE; RNA 16S;

ANOXIC CONDITIONS; BACTERIUM; ENZYME ACTIVITY; FERMENTATION; FLOW CYTOMETRY; GENE; HYBRIDIZATION; HYDROGEN; POLYMERASE CHAIN REACTION; RNA;

ANAEROBIC BACTERIUM; ARTICLE; BACILLUS; BACTERIAL STRAIN; BACTERIUM; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; BIOSYNTHESIS; CLOSTRIDIUM; CLOSTRIDIUM BUTYRICUM; CLOSTRIDIUM PASTEURIANUM; CLOSTRIDIUM SACCHAROBUTYLICUM; COMPARATIVE STUDY; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE EXPRESSION; GENE TARGETING; INOCULATION; NONHUMAN; NUCLEOTIDE SEQUENCE;

ANAEROBIOSIS; BASE SEQUENCE; CLOSTRIDIUM; FLOW CYTOMETRY; HYDROGEN; HYDROGENASE; IN SITU HYBRIDIZATION, FLUORESCENCE; MOLECULAR SEQUENCE DATA;

BACILLUS THERMOAMYLOVORANS; BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM BUTYRICUM; CLOSTRIDIUM PASTEURIANUM; CLOSTRIDIUM SACCHAROBUTYLICUM;

EID: 33947220032     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0740-8     Document Type: Article

References (23)

1. Amann RI, Binder BJ, Olsen RJ, Chrisholm SW, Devereux R, Stahl DA (1990) Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed populations. Appl Environ Microbiol 56:1919-1925
2. Auling G, Pilz F, Busse HJ, Karrasch S, Streichan M, Schön G (1991) Analysis of the polyphosphate-accumulating microflora in phosphorus-eliminating, anaerobic-aerobic activated sludge systems by using diaminopropane as a biomarker for rapid estimation of Acinetobacter spp. Appl Environ Microbiol 57:3585-3592
3. Cammack R (1999) Hydrogenase sophistication. Nature 397:214-215
4. Chang JJ, Chen WE, Shih SY, Yu SJ, Lay JJ, Wen FS, Huang CC (2006) Molecular detection of the clostridia in an anaerobic biohydrogen fermentation system by hydrogenase mRNA-targeted reverse transcription-PCR. Appl Microbiol Biotechnol 70:598-604
5. Chen WM, Tseng ZJ, Lee KS, Chang JS (2005) Fermentative hydrogen production with Clostridium butyricum CGS5 isolated from anaerobic sewage sludge. Int J Hydrogen Energy 30:1063-1070
6. Cloete TE, Steyn PL (1988) A combined membrane filter-immunofluorescent technique for the in situ identification and enumeration of Acinetobacter in activated sludge. Water Res 22:961-969
7. Devereux R, Mundfrom GW (1994) A phylogenetic tree of 16S rRNA sequences from sulfate-reducing bacteria in sandy marine sediment. Appl Environ Microbiol 60:3437-3439
8. Esteso MA, Estrella CN, Podestá JJ (1996) Evaluation of the absorption on mild steel of hydrogen evolved in glucose fermentation by pure cultures of Clostridium acetobutylicum and Enterobacter. Sens Actuators B Chem 32:27-31
9. Fang HH, Zhang T, Liu H (2002) Microbial diversity of a mesophilic hydrogen-producing sludge. Appl Microbiol Biotechnol 58:112-118
10. Gorwa MF, Croux C, Soucaille P (1996) Molecular characterization and transcriptional analysis of the putative hydrogenase gene of Clostridium acetobutylicum ATCC 824. J Bacteriol 178:2668-2675
11. Hiraishi A (1988) Respiratory quinone profiles as tools for identifying different bacterial populations in activated sludge. J Gen Appl Microbiol 34:39-56
12. Howgrave-Graham AR, Steyn PL (1988) Application of the fluorescent-antibody technique for the detection of Sphaerotilus natans in activated sludge. Appl Environ Microbiol 54:799-802
13. Karube I, Urano N, Matsunaga T, Suzuki S (1982) Hydrogen production from glucose by immobilized growing cells of Clostridium butyricum. Eur J Appl Microbiol 16:5-9
14. Kataoka N, Miya A, Kiriyama K (1997) Studies on hydrogen production by continuous culture system of hydrogen-producing anaerobic bacteria. Water Sci Technol 36:41-47
15. Lay JJ (2000) Modeling and optimization of anaerobic digested sludge converting starch to hydrogen. Biotechnol Bioeng 68:269-278
16. Lay JJ (2001) Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose. Biotechnol Bioeng 74:280-287
17. Muyzer G, de Waal EC, Uitterlinden AG (1993) Profiling of complex microbial population by DGGE analysis of polymerase chain reaction amplified genes encoding for 16S rRNA. Appl Environ Microbiol 62:2676-2680
18. Nielsen AT, Liu WT, Filipe C, Grady L, Molin S, Stahl DA (1999) Identification of a novel group of bacteria in sludge from a deteriorated biological phosphorus removal reactor. Appl Environ Microbiol 65:1251-1258
19. Palmer CJ, Tsai YL, Paszko-Kolva C, Mayer C, Sangermano LR (1993) Detection of Legionella species in sewage and ocean water by polymerase chain reaction, direct fluorescent-antibody, and plate culture methods. Appl Environ Microbiol 59:3618-3624
20. Snaidr J, Amann R, Huber I, Ludwig W, Schleifer KH (1997) Phylogenetic analysis and in situ identification of bacteria in activated sludge. Appl Environ Microbiol 63(7):2884-2896
21. Ueno Y, Otauka S, Morimoto M (1996) Hydrogen production from industrial wastewater by anaerobic microflora in chemostat culture. J Ferment Bioeng 82:194-197
22. Völsch A, Nader WF, Geiss HK, Nebe G, Birr C (1990) Detection and analysis of two serotypes of ammonia-oxidizing bacteria in sewage plants by flow-cytometry. Appl Environ Microbiol 56:2430-2435
23. Zhang T, Fang HP (2000) Digitization of DGGE (denaturing gradient gel electrophoresis) profile and cluster analysis of microbial communities. Biotechnol Lett 22:399-405