Stable expression of adenylyl cyclase 2 leads to the functional rescue of human 5-HT6 receptor in a CHODUKX cell line

Journal of Pharmacological and Toxicological Methods

Volumn 55, Issue 3, 2007, Pages 323-331

  Nawoschik, Stanley P ^a   Olsen, Michael ^a   Smith, Deborah L ^a   Khawaja, Xavier ^a  

^a CN8000   (United States)

Author keywords

Adenylyl cyclase; cAMP; CHODUKX; Human 5 HT6; Methods; pHTop vector plasmid

Indexed keywords

4 (3 BUTOXY 4 METHOXYBENZYL) 2 IMIDAZOLIDINONE; ADENYLATE CYCLASE; BISINDOLYLMALEIMIDE; CLOZAPINE; G PROTEIN COUPLED RECEPTOR; LISURIDE; MESULERGINE; METITEPINE; MIANSERIN; PHORBOL 13 ACETATE 12 MYRISTATE; SEROTONIN; SEROTONIN 6 RECEPTOR; SUMATRIPTAN;

ANIMAL CELL; ARTICLE; BINDING SITE; CELL FUNCTION; CONTROLLED STUDY; DOSE RESPONSE; HUMAN; HUMAN CELL; LIGAND BINDING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; RAT; SEROTONIN RELEASE; SIGNAL TRANSDUCTION;

ADENYLATE CYCLASE; BLOTTING, WESTERN; CELL LINE; CLONE CELLS; CYCLIC AMP; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG DESIGN; DRUG EVALUATION, PRECLINICAL; GENE EXPRESSION REGULATION; HUMANS; ISOENZYMES; LIGANDS; PLASMIDS; RADIOLIGAND ASSAY; RECEPTORS, SEROTONIN; SEROTONIN AGONISTS; SIGNAL TRANSDUCTION; TETRAHYDROFOLATE DEHYDROGENASE; TRANSFECTION;

RATTUS;

EID: 33947209946     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vascn.2006.10.003     Document Type: Article

References (22)

1. 6 receptors stably expressed in HEK293 cells. Neuropharmacology 36 (1997) 713-720
2. 6 receptor. Journal of Neurochemistry 71 (1998) 2169-2177
3. 6 receptor in the rat brain: A study using antisense oligonucleotides. Journal of Pharmacology and Experimental Therapeutics 274 (1995) 173-180
4. Fisas A., Codony X., Romero G., Dordal A., Giraldo J., Merce R., et al. Chronic 5-HT(6) receptor modulation by E-6837 induces hypophagia and sustained weight loss in diet-induced obese rats. British Journal of Pharmacology 148 (2006) 973-983
5. Harry A., Chen Y., Magnusson R., Iyengar R., and Weng G. Differential regulation of adenylyl cyclases by Galphas. Journal of Biological Chemistry 272 (1997) 19017-19021
6. Hirst W.D., Abrahamsen B., Blaney F.E., Calver A.R., Aloj L., Price G.W., et al. Differences in the central nervous system distribution and pharmacology of the mouse 5-hydroxytryptamine-6 receptor compared with rat and human receptors investigated by radioligand binding, site-directed mutagenesis, and molecular modeling. Molecular Pharmacology 64 (2003) 1295-1308
7. 125)I]-SB-258585 binding to human recombinant and native 5-HT(6) receptors in rat, pig and human brain tissue. British Journal of Pharmacology 130 (2000) 1597-1605
8. s protein. Biochemical and Biophysical Research Communications 329 (2005) 684-692
9. Kaufman R.J., Davies M.V., Wasley L.C., and Michnick D. Improved vectors for the stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus. Nucleic Acids Research 19 (1991) 4485-4490
10. 6 serotonin receptor. Journal of Neurochemistry 66 (1996) 47-56
11. MacEwan D.J., Kim G.D., and Milligan G. Agonist regulation of adenylate cyclase activity in neuroblastoma x glioma hybrid NG108-15 cells transfected to co-express adenylate cyclase type II and the beta 2-adrenoceptor. Evidence that adenylate cyclase is the limiting component for receptor-mediated stimulation of adenylate cyclase activity. Biochemical Journal 318 (1996) 1033-1039
12. Mons N., Harry A., Dubourg P., Premont R.T., Iyengar R., and Cooper D.M. Immunohistochemical localization of adenylyl cyclase in rat brain indicates a highly selective concentration at synapses. Proceedings of the National Academy of Sciences of the United States of America 92 (1995) 8473-8477
13. o proteins in modulation of adenylyl cyclase type-2 activity. Journal of Neurochemistry 83 (2002) 1252-1261
14. Pieroni J.P., Harry A., Chen J., Jacobowitz O., Magnusson R.P., and Iyengar R. Distinct characteristics of the basal activities of adenylyl cyclases 2 and 6. Journal of Biological Chemistry 270 (1995) 21368-21373
15. 6 receptor. Synapse 47 (2003) 218-224
16. 6 receptor-mediated cAMP signaling pathways. British Journal of Pharmacology 148 (2006) 1133-1143
17. Schechter L.E., Ring R.H., Beyer C.E., Hughes Z.A., Khawaja X., Malberg J.E., and Rosenzweig-Lipson S. Innovative approaches for the development of antidepressant drugs: current and future strategies. NeuroRx 2 (2005) 590-611 (Review)
18. 6 receptors positively coupled to adenylyl cyclase in striatal neurones in culture. Neuroreport 5 (1994) 2553-2557
19. 6 receptors. British Journal of Pharmacology 124 (1998) 556-562
20. Vickers S.P., and Dourish C.T. Serotonin receptor ligands and the treatment of obesity. Current Opinion in Investigational Drugs 5 (2004) 377-388
21. 6 receptors in retention of spatial learning in the Morris water maze. Neuropharmacology 41 (2001) 210-219
22. 6 receptors. Current Drug Targets. CNS and Neurological Disorders 3 (2004) 59-79