Simple method for a cell count of the colonial cyanobacterium, Microcystis sp.

Journal of Microbiology

Volumn 44, Issue 5, 2006, Pages 562-565

  Joung, Seung Hyun ^a   Kim, Choong Jae ^a   Ahn, Chi Yong ^a   Jang, Kam Yong ^b   Sung, Min Boo ^c   Oh, Hee Mock ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Konyang University   (South Korea)

^c Chungnam National University   (South Korea)

Author keywords

Boiling method; Cell count; Colony volume; Cyanobacteria; Microcystis

Indexed keywords

CYANOBACTERIA; MICROCYSTIS; MICROCYSTIS SP.;

ALGORITHM; ARTICLE; BACTERIAL COUNT; CYTOLOGY; METHODOLOGY; MICROCYSTIS; SPECIES DIFFERENCE; TEMPERATURE;

ALGORITHMS; COLONY COUNT, MICROBIAL; MICROCYSTIS; SPECIES SPECIFICITY; TEMPERATURE;

EID: 33947118842     PISSN: 12258873     EISSN: 12258873     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Asai, R., S. McNiven, K. Ikebukuro, and I. Karube. 2000. Development of a fluorometric sensor for the measurement of phycobilin pigment and application to freshwater phytoplankton. Field Anal. Chem. Technol. 4, 53-61.
2. Dubelaar, G.B.J. and C.S. van der Reijden. 1995. Size distributions of Microcystis aeruginosa colonies: A flow cytometric approach. Water Sci. Tech. 32, 171-176.
3. Falconer, I.R. 1994. Health problems from exposure to cyanobacteria and proposed safety guidelines for drinking and recreational water, p. 3-10. In G.A. Codd, T.M. Jefferies, C.W. Keevil, and E. Potter (eds.), Detection methods for cyanobacterial toxins. The Royal Society of Chemistry, Cambridge, UK
4. Ishikawa, K., R.F. Walker, S. Tsujimura, H. Nakahara, and M. Kumagai. 2004. Estimation of Microcystis colony size in developing water blooms via image analysis. J. Japan Society on Water Environ. 27, 69-72. (In Japanese).
5. Kurmayer, R., G. Christiansen, and I. Chorus. 2003. The abundance of microcystin-producing genotypes correlates positively with colony size in Microcystis sp. and determines its microcystin net production in Lake Wannsee. Appl. Environ. Microbiol. 69, 787-795.
6. Lyck, S. 2004. Simultaneous changes in cell quotas of microcystin, chlorophyll a, protein and carbohydrate during different growth phases of a batch culture experiment with Microcystis aeruginosa. J. Plankton Res. 26, 727-736.
7. Manage, P.M., Z. Kawabata, and S. Nakano. 1999. Seasonal changes in densities of cyanophage infectious to Microcystis aeruginosa in a hypereutrophic pond. Hydrobiologia 411, 211-216.
8. Neilan, B.A., J. Daniel, and E.G. Amanda. 1995. Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol. 61, 3875-3883.
9. Oh, H.M. and G.Y. Rhee. 1990. Preparation of unialgal cultures from natural waters by a micropipette technique. Korean J. Phycol. 5, 131-136.
10. Oliver, R.L. and G.G. Ganf. 2000. Freshwater blooms, p. 149-194. In B.A. Whitton, and M. Potts (eds.), The ecology of cyanobacteria. Their diversity in time and space. Kluwer Academic Publishers, Dordrecht.
11. Otsuka, S., S. Suda, R. Li, M. Watanabe, H. Oyaizu, S. Matsumoto, and M.M. Watanabe. 1998. 16S rDNA sequences and phylogenetic analysis of Microcystis strains with and without phycoerythrin. FEMS Microbiol. Lett. 164, 119-124.
12. Reynolds, C.S., G.H.M. Jaworski, H.A. Cmiech, and G.F. Leedale. 1981. On the annual cycle of the blue-green alga Microcystis aeruginosa Kütz Emend Elenkin. Phil. Trans. R. Soc. Lond. B 293, 419-477.
13. Soranno, P.A. 1997. Factors affecting the timing of surface scums and epilimnetic blooms of blue-green algae in a eutrophic lake. Can. J. Fish. Aquat. Sci. 54, 1965-1975.
14. Takamura, N. and M. Watanabe. 1987. Seasonal changes in the biomass of four species of Microcystis in Lake Kasumigaura. Jap. J. Limnol. 48, S139-S144 (In Japanese).
15. Tsujimura, S. 2003. Application of the frequency of dividing cells technique to estimate the in situ growth rate of Microcystis (Cyanobacteria). Freshwat. Biol. 48, 2009-2024.
16. Via-Ordorika, L., J. Fastner, R. Kurmayer, M. Hisbergues, E. Dittmann, J. Komarek, M. Erhard, and I. Chorus. 2004. Distribution of microcystin-producing and non-microcystin-producing Microcystis sp. in European freshwater bodies: Detection of microcystins and microcystin genes in individual colonies. System. Appl. Microbiol. 27, 592-602.
17. Welker, M., M. Brunke, K. Preussel, I. Lippert, and H.V. Döhren. 2004. Diversity and distribution of Microcystis (cyanobacteria) oligopeptide chemotypes from natural communities studied by single-colony mass spectrometry. Microbiology 150, 1785-1796.
18. Whang, G.D. and Y.S. So. 1993. Photochemical removal of color in aqueous suspension of titanium dioxide. J. Kor. Soc. Waste Manage. 10, 325-331 (In Korean).