Nested reverse transcription polymerase chain reaction for the detection of rubella virus in clinical specimens

Indian Journal of Medical Research

Volumn 125, Issue 1, 2007, Pages 73-78

  Shyamala, G ^a   Malathi, J ^a   Moses, Y S ^a   Therese, K L ^a   Madhavan, H N ^a  

^a VISION RESEARCH FOUNDATION   (India)

Author keywords

Congenital cataract; Congenital rubella syndrome; Lens aspirates; RT PCR; Rubella virus

Indexed keywords

GLYCOPROTEIN E1; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; VIRUS RNA;

ANTIBODY DETECTION; ARTICLE; CLINICAL ARTICLE; CONGENITAL CATARACT; CONGENITAL RUBELLA SYNDROME; CONTROLLED STUDY; DISEASE ASSOCIATION; HUMAN; HUMAN CELL; INFANT; INTERMETHOD COMPARISON; LEUKOCYTE; NONHUMAN; OPEN READING FRAME; PROCESS OPTIMIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; RUBELLA VIRUS; SENSITIVITY ANALYSIS; SEROLOGY; VERO CELL; VERTICAL TRANSMISSION; VIRUS CULTURE; VIRUS DETECTION; VIRUS ISOLATION; VIRUS STRAIN;

ANIMALS; CATARACT; CERCOPITHECUS AETHIOPS; HUMANS; INFANT; INFANT, NEWBORN; LENS, CRYSTALLINE; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; RUBELLA; RUBELLA VIRUS; VERO CELLS;

EID: 33947100002     PISSN: 09715916     EISSN: 09715916     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Gupta E, Dar L, Broor S. Seroprevalence of Rubella in pregnant women in Delhi; India. Indian J Med Res 2006; 123 : 833-5.
2. Angara SK. Aetiology and management of congenital cataract. Indian J Pediatr 1987; 54 : 673-7.
3. Eckstein M, Vijayalakshmi P, Killedar M, Gilbert C, Foster A. Aetiology of childhood cataract in south India. Br J Ophthalmol 1996; 80 : 628-32.
4. Givens KT, Lee DA, Jones T, Ilstrup DM. Congenital rubella syndrome ophthalmic manifestations and associated systemic disorders. Br J Ophthalmol 1993; 77 : 358-63.
5. Sridharan G, Ohn TS, Mathai E, Moses PD, Cherian T. Kuruvilla KA, et al. Serology in congenital infections: Experience in selected symptomatic infants. Indian Pediatr 1999; 36 : 667-9.
6. Anne GA. Rubella virus. In: Mandell GL, Bennet JE, Dolin R, editors. Mandell, Douglas and Bennett's principles and practice of infectious diseases. New York: Churchill Livingstone; 2005 p. 1921-4.
7. Best JM, O'Shea C. Rubella virus: diagnostic procedures for viral, Rickettsial and Chlamydial infections. 6th ed. Washington: American Public Health Association; 1989 p. 731-95.
8. Malathi J, Therese KL, Madhavan HN. The association of Rubella virus in congenital cataract - a hospital-based study in India. J Clin Virol 2001; 23 : 25-9.
9. Belleni WJ, Sever JL. Measles, mumps & rubella. In: Specter S, Hodinka RL, Young SA, editors. Clinical virology manual. Washington DC: ASM Press; 2000 p. 507-9.
10. Bosma TJ, Corbett KM, Eckstein MB, O'Shea S, Vijayalakshmi P, Banatvala JE, et al. Use of PCR for prenatal & post natal diagnosis of congenital rubella. J Clin Microbiol 1995; 33 : 2881-7.
11. Tanemura M, Suzumori K, Yagami Y, Katow S. Diagnosis of fetal rubella infection with reverse transcription & nested polymerase chain reaction: a study of 34 cases diagnosed in fetusus. Am J Obstet Gynaecol 1996; 174 : 578-82.
12. Bosma TJ, Corbett KM, O'Shea S, Banatvala JE, Best JM. PCR for detection of Rubella virus RNA in clinical samples. J Clin Microbiol 1995; 33 : 1075-9.
13. Revello MG, Baldanti F, Sarasini A, Zavattoni M, Torsellini M, Gerna G. Prenatal diagnosis of Rubella virus infection by direct detection and semiquantitation of viral RNA in clinical samples by reverse transcription-PCR. J Clin Microbiol 1997; 35 : 708-13.
14. Vyse AJ, Jin L. An RT-PCR assay using oral fluid samples to detect rubella virus genome for epidemiological surveillance. Mol Cell Probes 2002; 16 : 93-7.
15. Macé M, Cointe D, Six C, Levy-Bruhl D, Parent du Chatelet I, Ingrand D, et al. Diagnostic value of reverse transcription-PCR of amniotic fluid for prenatal diagnosis of congenital rubella infection in pregnant women with confirmed primary rubella infection. J Clin Microbiol 2004; 42 : 4818-20.
16. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem 1987; 162 : 156-9.
17. Eckstein MB, Brown DWG, Foster A, Richards AF, Gilbert C E, Vijayalakshmi P. Congenital rubella in south India: diagnosis using saliva from infants with cataract. Br Med J 1996; 312 : 161.