Nucleolus disassembly in mitosis and apoptosis: Dynamic redistribution of phosphorylated-c-Myc, fibrillarin and Ki-67

European Journal of Histochemistry

Volumn 50, Issue 4, 2006, Pages 273-280

  Soldani, Cristiana ^a   Bottone, M G ^a,b   Pellicciari, C ^a   Scovassi, A I ^b  

^a UNIVERSITY OF PAVIA   (Italy)

^b CNR   (Italy)

Author keywords

Apoptosis; Fibrillarin; Ki 67; Mitosis; Nucleolus; Phosphorylated c Myc

Indexed keywords

FIBRILLARIN; KI 67 ANTIGEN; DNA BINDING PROTEIN; MYCBP PROTEIN, HUMAN; NONHISTONE PROTEIN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ANTIBODY LABELING; APOPTOSIS; ARTICLE; CELL PROLIFERATION; CYTOPLASM; FLUORESCENCE MICROSCOPY; HUMAN; IMMUNOCYTOCHEMISTRY; KINETICS; MITOSIS; PHOSPHORYLATION; WESTERN BLOTTING; CONFOCAL MICROSCOPY; FLUORESCENT ANTIBODY TECHNIQUE; HELA CELL; METABOLISM; NUCLEOLUS; PHYSIOLOGY;

APOPTOSIS; BLOTTING, WESTERN; CELL NUCLEOLUS; CHROMOSOMAL PROTEINS, NON-HISTONE; DNA-BINDING PROTEINS; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HELA CELLS; HUMANS; KI-67 ANTIGEN; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MITOSIS; PHOSPHORYLATION; TRANSCRIPTION FACTORS;

EID: 33847784959     PISSN: 1121760X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Amati B. Myc degradation: dancing with ubiquitin ligases. Proc Natl Acad Sci USA 2004; 101:8843-44.
2. Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, et al. Nucleolar proteome dynamics. Nature 2005; 433:77-8.
3. Arabi A, Wu S, Ridderstrale K, Bierhoff H, Shiue C, Fatyol K, et al. c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription. Nat Cell Biol 2005; 7:303-10.
4. Biggiogera M, Bottone MG, Scovassi AI, Soldani C, Vecchio L, Pellicciari C. Rearrangement of nuclear RNP-containing structures during apoptosis and transcriptional arrest. Biol Cell 2004; 96:603-15.
5. Biggiogera M, Pellicciari C. Heterogeneous ectopic RNP-derived structures (HERDS) are markers of transcriptional arrest. FASEB J 2000; 14:828-34.
6. Brown DC, Gatter KC. Ki67 protein: the immaculate deception? Histopathology 2002; 40:2-11.
7. Cline AM, Radic MZ. Apoptosis, subcellular particles, and autoimmunity. Clin Immunol 2004; 112:175-82.
8. Donzelli M, Bernardi R, Negri C Prosperi E, Padovan L, Lavialle C, Brison O, et al. Apoptosis-prone phenotype of human colon carcinoma cells with a high level amplification of the c-myc gene. Oncogene 1999; 18:439-48.
9. Endl E, Gerdes J. The Ki-67 Protein: Fascinating forms and an unknown function. Exp Cell Res 2000; 257:231-37.
10. Endl E, Hollmann C, Gerdes J. Antibodies against the Ki-67 protein: assessment of the growth fraction and tools for cell cycle analysis. Methods Cell Biol 2001; 63:399-418.
11. Fraschini A, Bottone MG, Scovassi AI, Denegri M, Risueno MC, Testillano PS, et al. Changes in extranucleolar transcription during actinomycin D-induced apoptosis. Histol Histopathol 2005; 20:107-17.
12. Gébrane-Younès J, Sirri V, Junéra HR, Roussel P, Hernandez-Verdun D. Nucleolus: an essential nuclear domain. In: Hemmerich P, Diekmann S, eds. Visions of the cell nucleus, American Scientific Publishers, CA, 2005, pp. 120-35.
13. Goessens G. Nucleolar structure. Int Rev Cytol 1984; 87:107-158.
14. Halicka HD, Bedner E, Darzynkiewicz Z. Segregation of RNA and separate packaging of DNA and RNA in apoptotic bodies during apoptosis. Exp Cell Res 2000; 260:248-56.
15. Hernandez-Verdun D, Gautier T. The chromosome periphery during mitosis. Bioessays 1994; 16:179-85.
16. Hernandez-Verdun D. Nucleolus: from structure to dynamics. Histochem Cell Biol 2006; 125:127-37.
17. Horky M, Kotala V, Anton M, Wesierska-Gadek J. Nucleolus and apoptosis. Ann NY Acad Sci 2002; 973:258-64.
18. Martelli AM, Robuffo I, Bortul R, Ochs RL, Luchetti F, Cocco L, et al. Behavior of nucleolar proteins during the course of apoptosis in camptothecin-treated HL60 cells. J Cell Biochem 2000; 78:264-77.
19. Navarrete M, Maier CM, Falzoni R, Quadros LG, Lima GR, Baracat EC, et al. Assessment of the proliferative, apoptotic and cellular renovation indices of the human mammary epithelium during the follicular and luteal phases of the menstrual cycle. Breast Cancer Res 2005; 7:306-13.
20. Olson MO, Dundr M. The moving parts of the nucleolus. Histochem Cell Biol 2005; 123:203-16.
21. Oskarsson T, Trumpp A. The Myc trilogy: lord of RNA polymerases. Nat Cell Biol 2005; 7:215-17.
22. Pelengaris S, Khan M, Evan G. c-MYC: more than just a matter of life and death. Nat Rev Cancer 2002; 2:764-76.
23. Pellicciari C, Bottone MG, Soldani C, Vecchio L, Biggiogera M. Extrusion of nuclear protein during apoptosis. In: Scovassi AI, ed. Apoptosis. Kerala, India: Research Signpost, 2005, pp. 131-44.
24. Ross W, Hall PA. Ki67: from antibody to molecule to understanding? J Clin Pathol Mol Pathol 1995; 48:113-17.
25. Rubbi CP, Milner J. Disruption of the nucleolus mediates stabilization of p53 in response to DNA damage and other stresses. EMBO J 2003; 22:6068-77.
26. Scheer U, Benavente R. Functional and dynamic aspects of the mammalian nucleolus. Bioessays 1990; 12:14-21.
27. Secombe J, Pierce SB, Eisenman RN. Myc: a weapon of mass destruction. Cell 2004; 117:153-56.
28. Smith KP, Byron M, O'Connell BC, Tam R, Schorl C, Guney I, et al. c-Myc localization within the nucleus: evidence for association with the PML nuclear body. J Cell Biochem 2004; 93:1282-96.
29. Soldani C, Bottone MG, Biggiogera M Alpini C, Scovassi AI, Martin T, Pellicciari C. Nuclear localization of phosphorylated c-Myc protein in human tumor cells. Eur J Histochem 2002; 46:377-80.
30. Thiry M, Goessens G. The nucleolus during the cell cycle. Heidelberg, Germany: Springer-Verlag GmbH & Co KG, 2004, pp. 30-36.
31. Vervoorts J, Lüscher-Firzlaff J, Lüscher B. The Ins and Outs of MYC Regulation by Posttranslational Mechanisms. J Biol Chem 2006; 281:34725-29.
32. Zimber A, Nguyen Q-D, Gespach C. Nuclear bodies and compartments: functional roles and cellular signalling in health and disease. Cell Signal 2004; 16:1085-104.