Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana

Biotechnology Letters

Volumn 29, Issue 4, 2007, Pages 569-574

  Hong, Young Ho ^a   Lee, Dong Woo ^b   Lee, Sang Jae ^a   Choe, Eun Ah ^c   Kim, Seong Bo ^d   Lee, Yoon Hee ^e   Cheigh, Chan Ick ^a   Pyun, Yu Ryang ^a  

^a Yonsei University   (South Korea)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d CJ Corporation   (South Korea)

^e OHIO STATE UNIVERSITY   (United States)

Author keywords

D Tagatose; Esherichia coli; Immobilization; L Arabinose isomerase; Thermotoga neapolitana

Indexed keywords

CALCIUM COMPOUNDS; CELLS; ENZYME IMMOBILIZATION; ESCHERICHIA COLI;

CALCIUM ALGINATE BEADS; RECYCLING MODES; TAGATOSES; THERMOTOGA NEAPOLITANA;

ENZYME KINETICS;

ARABINOSE; HEXOSE; ISOMERASE; RECOMBINANT PROTEIN; SWEETENING AGENT; TAGATOSE;

ARTICLE; BIOSYNTHESIS; CULTURE TECHNIQUE; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING; TEMPERATURE; THERMOTOGA NEAPOLITANA;

ARABINOSE; CELL CULTURE TECHNIQUES; ESCHERICHIA COLI; HEXOSES; ISOMERASES; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SWEETENING AGENTS; TEMPERATURE; THERMOTOGA NEAPOLITANA;

ESCHERICHIA COLI; THERMOTOGA NEAPOLITANA;

EID: 33847723004     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9277-2     Document Type: Article

References (20)

1. Adachi S, Sugawara H (1963) Separation of ketose and aldose by chromatography on an ion-exchange column. Arch Biochem Biophys 100:468-471
2. Beadle JR, Saunders JP, Wajda TJ (1992) Process for manufacturing tagatose. U.S. Patent 5,078,796
3. Busto MD (1998) An experiment illustrating the effect of immobilization on enzyme properties. Biochem Educ 26:304-308
4. Cheetham PSJ, Wootton AN (1992) Bioconversion of D-galactose into D-tagatose. Enzyme Microb Technol 15:105-108
5. Dische Z, Borefreund E (1951) A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem 192:583-387
6. Ibrahim OO, Spradlin JE (2000) Process for manufacturing D-tagatose. U.S. Patent 6,057,135
7. Jørgensen F, Hansen OC, Stougaard P (2004) Enzymatic conversion of D-galactose to D-tagatose:heterologous expression and characterization of a thermostable L-arabinose isomerase from Thermoanaerobacter mathranii. Appl Microbiol Biotechnol 64:816-822
8. Kim BC, Lee YH, Lee HS, Lee DW, Choe EA, Pyun YR (2002) Cloning, expression and characterization of L-arabinose isomerase from Thermotoga neapolitana: bioconversion of D-galactose to D-tagatose using the enzyme. FEMS Microbiol Lett 212:121-126
9. Lee DW, Jang HJ, Choe EA, Kim BC, Lee SJ, Kim SB, Hong YH, Pyun YR (2004) Characterization of a thermostable L-arabinose isomerase (D-galactose) isomerase from the hyperthermophilic eubacterium Thermotoga maritima. Appl Environ Microbiol 70:1397-1404
10. Lee DW, Choe EA, Kim SB, Eom SH, Hong YH, Lee SJ, Lee HS, Lee DY, Pyun YR (2005a) Distinct metal dependence for catalytic and structural functions in the L-arabinose isomerases from the mesophilic Bacillus halodurans and the thermophilic Geobacillus stearothermophilius. Arch Biochem Biophys 434:333-343
11. Lee DW, Hong YH, Choe EA, Lee SJ, Kim SB, Lee HS, Oh JW, Shin HH, Pyun YR (2005b) A thermodynamic study of mesophilic, thermophilic, and hyperthermophilic L-arabinose isomerases: the effects of divalent metal ions on protein stability at elevated temperatures. FEBS Lett 579:1261-1266
12. Lernia ID, Schiraldi C, Generoso M, Rosa MD (2002) Trehalose production at high temperature exploiting an immobilized cell reactor. Extremophiles 6:341-347
13. Levin GV (2002) Tagatose, the new GRAS sweetener and health product. J Med Food 5:23-36
14. Nabors LO (2002) Sweet choices:sugar replacements for foods and beverages. Food Technol 56:28-45
15. Ortega N, Busto MD, Perez-Mateos M (1998) Stabilization of β-glucosidase entrapped in alginate and polyacrylamide gels towards thermal and proteolytic deactivation. J Chem Technol Biotechnol 73:7-12
16. Ryu SA, Kim CS, Kim HJ, Baek DH, Oh DK (2003) Continuous D-tagatose production by immobilized thermostable L-arabinose isomerase in a packed-bed bioreactor. Biotechnol Prog 19:1643-1647
17. Shaw CJ, Huang A, Zhang X (2003) Quantitation and test of enantiomeric purity of the L-ketohexoses by liquid chromatography with dual refractive index and laser-based chiroptical detection. J Chromatogr A 987:439-443
18. Tewari YB, Goldberg RN (1985) An investigation of the equilibria between aqueous ribose, ribulose, and arabinose. Biophys Chem 22:197-204
19. Tewari YB, Steckler DK, Goldberg RN (1985) Thermodynamics of the conversion of aqueous xylose to xylulose. Biophys Chem 22:181-185
20. Zehner LR (1988) D-Tagatose as a low-calorie carbohydrate sweetener and bulking agent. U.S. Patent 4,786,722