A potential generic downstream process using Cibracon Blue resin at very high loading capacity produces a highly purified monoclonal antibody preparation from cell culture harvest

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 848, Issue 1, 2007, Pages 108-115

  Riske, F ^a   Smith, M ^a   Menon, M ^a   Goetschalck, S ^b   Goidsenhoven, I V ^b   Krul, A ^b   Pimpaneau, V ^b   Renaers, I ^b   Van Tichelt, N ^b   Van Houdt, K ^b   Hayes, M ^a   Lawrence, C ^a   Bigelow, R ^a   Schroeder, J ^a  

^a GENZYME CORPORATION   (United States)

^b Genzyme Corporation   (United States)

Author keywords

Cibracon Blue resin; Generic downstream process; Monoclonal antibody; Purification of cell culture harvest

Indexed keywords

FEED STREAMS; GENERIC DOWNSTREAMS; POLYCYCLIC ANIONIC LIGANDS; TANGENTIAL FLOW FILTRATION (TFF);

CELL CULTURE; CHROMATOGRAPHY; DYES; HYDROPHOBICITY; MONOCLONAL ANTIBODIES; PURIFICATION;

RESINS;

CELL PROTEIN; CIBRACON BLUE; DYE; IMMUNOGLOBULIN G4 MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY; OLIGOMER; PROTEIN A; RESIN; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; CHROMATOGRAPHY; DOWNSTREAM PROCESSING; DRUG MANUFACTURE; DRUG PURIFICATION; DRUG PURITY; DRUG SYNTHESIS; FILTRATION; HOST CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL LINE, TUMOR; CULTURE MEDIA, CONDITIONED; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMUNOGLOBULIN G; RESINS, SYNTHETIC;

EID: 33847634400     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2006.06.034     Document Type: Article

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