Calcium ion-induced stabilization and refolding of agkisacutacin from agkistrodon acutus venom studied by fluorescent spectroscopy

Journal of Fluorescence

Volumn 17, Issue 2, 2007, Pages 215-221

  Xu, Xiaolong ^a   Chen, Jiexia ^a   Zhang, Liyun ^a   Wang, Shouye ^b   Shen, Dengke ^a   Liu, Qingliang ^a  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Agkisacutacin; Agkistrodon acutus; Calcium ion; Fluorescence; Unfolding

Indexed keywords

AGKISTRODON ACUTUS; ANTICOAGULANTS; DENATURANTS; STRUCTURAL STABILITY;

CALCIUM; CONFORMATIONS; DENATURATION; FLUORESCENCE; PLATELETS; POSITIVE IONS; PROTEINS;

TOXIC MATERIALS;

AGKISACUTACIN; CALCIUM; DIVALENT CATION; SNAKE VENOM; UNCLASSIFIED DRUG;

AGKISTRODON; ANIMAL; ARTICLE; CHEMISTRY; METABOLISM; PROTEIN DENATURATION; PROTEIN FOLDING; SPECTROFLUOROMETRY;

AGKISTRODON; ANIMALS; CALCIUM; CATIONS, DIVALENT; CROTALID VENOMS; PROTEIN DENATURATION; PROTEIN FOLDING; SPECTROMETRY, FLUORESCENCE;

AGKISTRODON; DEINAGKISTRODON ACUTUS;

EID: 33847631160     PISSN: 10530509     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10895-007-0160-x     Document Type: Article

References (28)

1. Morita T (2005) Structures and functions of snake venom CLPs (C-type lectin-like proteins) with anticoagulant-, procoagulant-, and platelet-modulating activities. Toxicon 45:1099-1114
2. Chen X, Qian Y, Liu Q, Li BX, Zhang M, Liu J (1999) Purification, characterization, and cDNA cloning of a new fibrinogenlytic venom protein, Agkisacutacin, from Agkistrodon acutus venom. Biochem Biophys Res Commun 265:530-535
3. Cheng X, Xu ZY, Liu QD, Li XM, Li XY, Liu J (2000) Purification and characterization of a platelet agglutinating inhibition protein (agkisacutacin) from Agkisacutacin acutus venom. Acta Biochim Biophys Sin 32:653-657
4. 2+ in fibrinolytic principle separated from snake venom. Spectrochimica Acta Part A 56:1843-1849
5. Li WF, Chen L, Li XM, Liu J (2005) A C-type lectin-like protein from Agkistrodon acutus venom binds to both platelet glycoprotein Ib and coagulation factor IX/factor X. Biochem Biophys Res Commun 332:904-912
6. Rumfeldt JAO, Stathopulos PB, Chakrabarrty A, Lepock JR, Meiering EM (2006) Mechanism and thermodynamics of guanidinium chloride-induced denaturation of LS-associated mutant Cu,Zn superoxide dismutases. J Mol Biol 355:106-123
7. Brun L, Isom DG, Velu P, Garcia-Moreno B, Royer CA (2006) Hydration of the folding transition state ensemble of a protein. Biochemistry 45:3473-348
8. Valente-Mesquita VL, Botelho MM, Ferreira ST (1998) Pressure-induced subunit dissociation and unfolding of dimeric beta-lactoglobulin. Biophys 175:471-476
9. Scalley ML, Baker D (1997) Protein folding kinetics exhibit an Arrhenius temperature dependence when corrected for the temperature dependence of protein stability. Proc Natl Acad Sci USA 94:10636-10640
10. Bedell JL, McCrary BS, Edmondson SP, Shriver JW (2000) The acid-induced folded state of Sac7d is the native state. Protein Sci 9:1878-1888
11. Liu XQ, Wang CC (2001) Disulfide-dependent folding and export of Escherichia coli DsbC. J Biol Chem 276:1146-1151
12. Ahmad A, Akhtar MS, Bhakuni V (2001) Monovalent cationinduced conformational change in glucose oxidase leading to stabilization of the enzyme. Biochemistry 40:1945-1955
13. Yuan C, Xie ZQ, Zhang FW, Xu GJ (2001) Association and activation of fructose 1,6-bisphosphase during unfolding and refolding: spectroscopic and enzymatic studies. J Protein Chem 20:39-47
14. Andersson D, Hammarstrom P, Carlsson U (2001) Cofactorinduced refolding: refolding of molten globule carbonic anhydrase induced by Zn(II) and Co(II). Biochemistry 40:2653-2661
15. Xu XL, Liu QL, Xie YS (2002) Metal ion-induced stabilization and refolding of anticoagulation factor II from the venom of Agkistrodon acutus. Biochemistry 41:3546-3554
16. Xu XL, Liu QL, Yu HM, Xie YS (2002) Ca(II) and Tb(III)-induced stabilization and refolding of anticoagulation factor I from the venom of Agkistrodon acutus. Protein Sci 11:944-956
17. Nozaki Y (1972) The preparation of guanidine hydrochloride. Methods Enzymol 26:43-50
18. Muzammil S, Kumar Y, Tayyab S (2000) Anion-induced stabilization of human serum albumin prevents the formation of intermediate during urea denaturation. Proteins Struct Funct Genet 40:29-38
19. Ionescu RM, Smith VF, O'Neill JC, Matthews CR (2000) Multistate equilibrium unfolding of Escherichia coli dihydrofolate reductase: thermodynamic and spectroscopic description of the native, intermediate, and unfolded ensembles. Biochemistry 39:9540-9550
20. Eftink MR (1991) Fluorescence techniques for studying protein structure. Methods Biochem Anal 35:127-205
21. Brustein EA, Vedenkina NS, Irkova MN (1973) Fluorescence and the location of tryptophan residues in protein molecules, Photochem. Photobiol 18:263-279
22. Sakai K, Sakurai K, Sakai M, Hoshino M, Goto Y (2000) Conformation and stability of thiol-modified bovine β-lactoglobulin. Protein Sci 9:1719-1729
23. Chen YL, Tsai IH (1996) Function and sequence characterization of coagulation factor IX/factor X-binding protein from the venom of Echis carinatus leucogaster. Biochemistry 35:5264-5271
24. Atoda H, Ishikawa M, Mizuno H, Morita T (1998) Coagulation factor X-binding protein from Deinagkistrodon acutus venom is a Gla domain-binding protein. Biochemistry 37:17361-17370
25. Mizuno H, Fujimoto Z, Koizumi M, Kano H, Atoda H, Morita T (1997) Structure of coagulation factor IX/factor X-binding protein, a heterodimer of C-type lectin domains. Nat Struct Biol 4: 438-441
26. Mizuno H, Fujimoto Z, Koizumi M, Kano H, Atoda H, Morita T (1999) Crystal structure of coagulation factor IX-binding protein from habu snake venom at 2.6 Å: implication of central loop swapping based on deletion in the linker region. J Mol Biol 289:103-112
27. Mizuno H, Atoda H, Morita T (1991) Crystallization and preliminary X-ray study of blood coagulation factor IX/factor X-binding protein with anticoagulant activity from habu snake venom. J Mol Biol 220:225-226
28. Liu XH, Xu XL, Chen JX, Liu WQ, Liu QL (2005) Effects of metal ions and an inhibitor on the fluorescence and activity of acutolysin A from Agkistrodon acutus venom. Indian J Biochem Biophys 42:100-105