Expression, refolding, and characterization of recombinant thrombopoietin/stem cell factor fusion protein in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 74, Issue 4, 2007, Pages 836-842

  Zang, Yuhui ^a   Zhang, Xu ^a,b   Jiang, Xiaoling ^a   Li, Haoran ^a   Zhu, Jie ^a   Zhang, Chi ^a   Peng, Wei ^a   Qin, Junchuan ^a  

^a NANJING UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Bacterial expression; Inclusion body; Megakaryopoiesis; Refolding; TPO SCF

Indexed keywords

AFFINITY CHROMATOGRAPHY; CELLS; ESCHERICHIA COLI; SOLUBILITY; UREA;

INCLUSION BODIES; MEGAKARYOPOIESIS; STEM CELL FACTORS (SCF); THROMBOPOIETINS;

ENZYMES;

BACTERIAL PROTEIN; BUFFER; HYBRID PROTEIN; NICKEL; STEM CELL FACTOR; THIOREDOXIN; THROMBIN; THROMBOPOIETIN; UREA;

CHROMATOGRAPHY; COLIFORM BACTERIUM; EXPERIMENTAL STUDY; GENE EXPRESSION; PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; CELL LINE; CELL PROLIFERATION; CONTROLLED STUDY; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FOLDING; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL LINE; CELL PROLIFERATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; HUMANS; INCLUSION BODIES; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; STEM CELL FACTOR; THROMBOPOIETIN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 33847332571     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0734-6     Document Type: Article

References (20)

1. Archimbaud E, Thomas X (1998) Thrombopoietic factors potentially useful in the treatment of acute leukemia. Leuk Res 22:1155-1164
2. Avanzi GC, Lista P, Giovinazzo B, Moniero R, Saglio G, Benetton G, Codd R, Cattoretti G, Pegoraro L (1988) Selective growth response to IL-3 of a human leukaemic cell line with megakaryoblastic features. Br J Haematol 69:359-365
3. Broudy C (1997) Stem cell factor and hematopoiesis. Blood 90:1345-1364
4. Cao P, Mei JJ, Diao ZY, Zhang SQ (2005) Expression, refolding, and characterization of human soluble BAFF synthesized in Escherichia coli. Protein Expr Purif 41:199-206
5. Charles A, Wherter M, Feng Y, Linda L et al (1999) Circular permutation of the granulocyte colony-stimulating factor receptor agonist domain of myelopoietin. Biochemistry 38:4564-4571
6. Curtis BM, Williams DE, Broxmeyer H et al (1991) Enhanced hematopoietic activity of a human granulocyte/macrophage colony-stimulating factor-interleukin 3 fusion protein. Proc Natl Acad Sci USA 88:5809-5813
7. Drayer AL, Boer AK, Los EL, Esselin, MT, Vellenga E (2005) Stem cell factor synergistically enhances thrombopoietin-induced STAT5 signaling in megakaryocyte progenitors through JAK2 and Src kinase. Stem Cells 23:240-251
8. Gu Z, Weidenhaupt M, Ivanova N, Pavlov M, Xu B, Su ZG, Janson JC (2002) Chromatographic methods for the isolation of, and refolding of proteins from, Escherichia coli inclusion bodies. Protein Expr Purif 25:174-179
9. Kitamura T, Tange T, Terasawa T, Chiba S, Kuwaki T, Miyagawa K, Piao Y, Miyazono K, Urabe A, Takaku F (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol 140:323-334
10. Langley KE, Mendiaz EA, Liu N, Narhi LO, Zeni L, Pareseghian C, Clogston M, Leslie CL, Pope I, Lu JA (1994) Properties of variant forms of human stem cell factor recombinantly expressed in Escherichia coli. Arch Biochem Biophys 311:55-61
11. Lilie TH, Schwarz E, Rudolph R (1998) Advances in refolding of proteins produced in E. coli. Curr Opin Biotechnol 9:497-501
12. Lok S, Kaushansky K, Holly.RD, Kuijper, JL, Lofton-Day.CE, Oort, PJ, Grant FJ, Heipel MD, Burkhead SK, Kramer JM (1994) Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo. Nature 369:565-568
13. Lu H, Zang Y, Ze Y, Zhu J, Chen T, Qin J (2005) Expression, refolding and characterization of a novel recombinant dual human stem cell factor. Protein Expr Purif 43:126-132
14. Matsumura I, Kanakura Y, Kato T et al (1995) Growth response of acute myeloblastic leukemia cells to recombinant human thrombopoietin. Blood 86:703-709
15. Takanori M, Michael DF, Yoshihiro S, Youko K, Tomoyuki O, Tadashi O, Tomoyuki T, Hideya O, Kinya, O, Takashi K, Hiroshi M, Ryota K (2000) Functional analysis of the C-terminal region of recombinant human thrombopoietin. J Biol Chem 275:12090-12094
16. Vadhan-Raj S, Broxmeyer HE, Andreeff MJ, Bandres C, Buescher E, Benjamin R, Papadopoulos NE, Burgess A, Patel S, Plager C, Hittelman WN, McAlister L, Garrison L, Williams DE (1995) In vivo biologic effects of PIXY321, a synthetic hybrid protein of recombinant human granulocyte-macrophage colony-stimulating factor and interleukin-3 in cancer patients with normal hematopoiesis: a phase I study. Blood 86:2098-2105
17. Vadhan-Raj S, Cohen V, Bueso-Ramos C (2005) Thrombopoietic growth factors and cytokines. Curr Hematol Rep 4:137-144
18. Wendling F, Maraskovsky E, Debili N, Florindo C, Teepe M, Titeux M, Methia N, Breton-Gorius J, Cosman D, Vainchenker W (1994) cMpl ligand is a humoral regulator of megakaryocytopoiesis. Nature 369:571-574
19. Williams JL, Pipia GG, Datta NS (1998) Thrombopoietin requires additional megakaryocyte-active cytokines for optimal ex vivo expansion of megakaryocyte precursor cells. Blood 91:4118-4126
20. Zang Y, Zhang X, Yuan D, Zhang Y, Zhu J, Lu H, Chang C, Qin J (2006) Expression, purification, and characterization of a novel recombinant fusion protein, rhTPO/SCF, in Escherichia coli. Protein Expr Purif 47:427-433