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Volumn 129, Issue 7, 2007, Pages 1838-1839

Direct electrochemistry of tetraheme cytochrome c554 from Nitrosomonas europaea: Redox cooperativity and gating

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME; HEME; HYDROXYLAMINE OXIDASE;

EID: 33847309794     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja065657k     Document Type: Article
Times cited : (29)

References (19)
  • 14
    • 33847267150 scopus 로고    scopus 로고
    • PFV was achieved using a PGSTAT 12 electrochemical analyzer (Ecochemie, with experiments conducted in a thermostated glass electrochemical cell fit with a calomel working electrode (Accumet) and a platinum wire counter electrode. Protein films were prepared by taking either freshly polished EPG electrodes or alkanethiol-modified polycrystalline gold electrodes and then soaking either electrode in a dilute (∼10 γM) protein solution of cyt c554 (prepared as described in ref 4) in pH 7.0 buffer composed of 20 mM MOPS. Electroactive protein films could be achieved by soaking for a few hours, but the best results were attained at 4 °C, overnight. Once prepared, electrodes were then used directly by rinsing the excess protein solution from the electrode surface and then placing the electrode tip in the prechilled electrochemical cell
    • 554 (prepared as described in ref 4) in pH 7.0 buffer composed of 20 mM MOPS. Electroactive protein films could be achieved by soaking for a few hours, but the best results were attained at 4 °C, overnight. Once prepared, electrodes were then used directly by rinsing the excess protein solution from the electrode surface and then placing the electrode tip in the prechilled electrochemical cell.
  • 16
    • 33847245268 scopus 로고    scopus 로고
    • If fitting is carried out using three distinct species and the high-potential feature has an assigned stoichiometry of two electrons (n, 2, fitted values of napp still reveal cooperativity, napp, 1.24; i.e, napp ≠ 2 Figure S1
    • app ≠ 2 (Figure S1).
  • 18
    • 0021763648 scopus 로고    scopus 로고
    • -1 (ref 11).
    • -1 (ref 11).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.