Effect of mitomycin-C on human foreskin fibroblasts used as feeders in human embryonic stem cells: Immunocytochemistry MIB1 score and DNA ploidy and apoptosis evaluated by flow cytometry

Cell Biology International

Volumn 31, Issue 3, 2007, Pages 269-278

  Nieto, A ^a   Cabrera, C M ^a   Catalina, P ^a   Cobo, F ^a   Barnie, A ^a   Cortes J L ^a   Barroso del Jesus, A ^a   Montes, R ^a   Concha, A ^a  

^a HOSPITAL UNIVERSITARIO VIRGEN DE LAS NIEVES   (Spain)

Author keywords

Apoptosis; DNA ploidy; Human feeder; Ki 67; Mitomycin C

Indexed keywords

CELL DNA; KI 67 ANTIGEN; MITOMYCIN C;

APOPTOSIS; ARTICLE; CELL ACTIVITY; CELL CYCLE ARREST; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CELL CYCLE S PHASE; CELL DEATH; CELL PROLIFERATION; CONTROLLED STUDY; EMBRYONIC STEM CELL; FIBROBLAST; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; INCUBATION TIME; MITOSIS INDEX; PLOIDY; PREPUCE;

ANIMALS; APOPTOSIS; CELL CYCLE; CELL PROLIFERATION; CELLS, CULTURED; COCULTURE TECHNIQUES; EMBRYONIC STEM CELLS; FEASIBILITY STUDIES; FIBROBLASTS; FLOW CYTOMETRY; FLUORESCENT DYES; FORESKIN; HUMANS; IMMUNOHISTOCHEMISTRY; KI-67 ANTIGEN; MALE; MICE; MITOMYCIN; PLOIDIES;

EID: 33847229505     PISSN: 10656995     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cellbi.2006.11.006     Document Type: Article

References (31)

1. Amit M., Margulets V., Segev H., Shariki K., Laevsky I., Coleman R., et al. Human feeder layers for human embryonic stem cells. Biol of Reprod 68 (2003) 2150-2156
2. Barlogie B., and Drewinko B. Lethal and cytokinetic effects of mitomycin C on cultured human colon cancer cells. Cancer Res. 40 (1980) 1973-1980
3. Bilko N.M., Votyakova I.A., Vasylovka S.V., and Bilko D.I. Characterization of the interactions between stromal and haematopoietic progenitor cells in expansion cell culture models. Cell Biol Int 29 (2005) 83-86
4. Caly M., Genin P., Ghuzlan A.A., Elie C., Freneaux P., Klijanienko J., et al. Analysis of correlation between mitotic index, MIB1 score and S-phase fraction as proliferation markers in invasive breast carcinoma. Methodological aspects and prognostic value in a series of 257 cases. Anticancer Res 24 (2004) 3283-3288
5. Chang-Qing X., Ge L., Ding Y., Jian W., Tian-Cheng L., and Guang-Xu L. Proliferation feeder cells support prolonged expansion of human embryonic stem cells. Cell Biol Int 9 (2005) 623-628
6. Cheng L., Hammond H., Ye Z., Zhan X., and Dravid G. Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture. Stem Cells 21 (2003) 131-142
7. Heng B.C., Liu H., and Cao T. Feeder cell density-a key parameter in human embryonic stem cell culture. In vitro Cell Dev Biol Anim 40 (2004) 255-257
8. Herbig U., and Sedivy J.M. Regulation of growth arrest in senescence: telomere damage is not the end of the story. Mech Ageing Dev 127 (2006) 16-24
9. Hovatta O., Mikkola M., Gertow K., Stromberg A., Inzunza J., Hreinsson J., et al. A culture system using foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum Reprod 18 (2003) 1-6
10. Kang S.G., Chung H., Yoo Y.D., Lee J.G., Choi Y.I., and Yu Y.S. Mechanism of growth inhibitory effect of mitomycin-C on cultured human retinal epithelial cells: apoptosis and cell cycle arrest. Curr Eye Res 22 (2001) 174-181
11. Kirsch D.G., and Kastan M.B. Tumor-suppressor p53: implications for tumor development and prognosis. J Clin Oncol 16 (1998) 3158-3168
12. Liu J., Choonjans L., Tielens S., Speleman F., Cornelissen M., De Sutter P., et al. Culturing in vitro produced blastocysts in sequential media promotes ES cell derivation. Mol Reprod Dev 73 (2006) 1017-1021
13. Ludwig T.E., Levenstein M.E., Jones J.M., Berggren W.T., Mitchen E.R., Frane J.L., et al. Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 24 (2006) 185-187
14. Mullen P. Flow cytometric DNA analysis of human cancer cell lines. In: Langon S.P. (Ed). Cancer cell culture: methods and protocols. Methods in molecular medicine vol. 88 (2004), Humana Press Inc 248-255
15. Perry A., Stafford S.L., Scheithauer B.W., Suman V.J., and Lohse C.M. The prognostic significance of MIB-1, p53, and DNA flow cytometry in completely resected primary meningiomas. Cancer 82 (1998) 2262-2269
16. Ponchio L., Duma L., Oliveiro B., Gibelli N., Pedrazzoli P., and Robustelli della Cuna G. Mitomycin C as an alternative to irradiation to inhibit the feeder layer growth in long-term culture assays. Cytotherapy 2 (2000) 281-286
17. Reubinoff B.E., Pera M.F., Fong C.Y., Trounson A., and Bongso A. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 18 (2000) 399-404
18. Richards M., Fong C.Y., Chan W.K., Wong P.C., and Bongso A. Human feeders support prolonged undifferentiated growth of human inner cell masses and embryonic stem cells. Nature 20 (2002) 1-4
19. Richards M., Tan S., Fong C.Y., Wong P., and Bongso A. Comparative evaluation of various human embryonic stem cells. Stem Cells 21 (2003) 546-556
20. Roy A., Krzykwa E., Lemieux R., and Neron S. Increased efficiency of gamma-irradiated versus mitomycin C-treated feeder cells for the expansion of normal human cells in long-term cultures. J Hematother Stem Cell Res 10 (2001) 873-880
21. Sehlmeyer U., Meister A., Beisker W., and Wobus A.M. Low mutagenic effects of mitomycin C in undifferentiated embryonic P19 cells are correlated with efficient cell cycle control. Mutat Res 354 (1996) 103-112
22. Spyratos F., Ferrero-Pous M., Trassard M., Hacene K., Phillips E., Tubiana-Hulin M., et al. Correlation between MIB-1 and other proliferation markers: clinical implications of the MIB-1 cutoff value. Cancer 94 (2002) 2151-2159
23. Stojkovic P., Lako M., Przyborski S., Stewart R., Armstrong L., Evans J., et al. Human serum matrix support undifferentiated growth human embryonic stem cells. Stem Cells 23 (2005) 895-902
24. Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., et al. Embryonic stem cell lines derived from human blastocysts. Science 282 (1998) 1145-1147
25. Tomasz M., Lipman R., Chowdary D., Pawlak J., Verdine G.L., and Nakanishi K. Isolation and structure of a covalent cross-link adduct between mitomycin C and DNA. Science 235 (1987) 1204-1208
26. Urruticoechea A., Smith I.E., and Dowsett M. Proliferation marker Ki-67 in early breast cancer. J Clin Oncol 23 (2005) 7212-7220
27. Van Engeland M., Nieland L.J.W., Ramaekers F.C.S., Schutte B., and Reutelingsperger C. Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry 31 (1998) 1-9
28. Vansudevan C., Fursov N., Maunder P., Cong M., Federici M., Haskins J.R., et al. Improving high-content-screening assay performance by using division-arrested cells. Assay Drug Dev Technol 3 (2005) 515-523
29. Vermes I., Haanen C., Steffens-Nakken H., and Reutelingsperger C. A novel assay for apoptosis flow cytometry detection of phosphatidylserine expression on early apoptotic cell using fluorescein labelled Annexin V. J Immunol Methods 184 (1995) 39-51
30. Vescovi A.L., and Snyder E.Y. Establishment and properties of neural stem cell clones: plasticity in vitro and in vivo. Brain Pathol 9 (1999) 569-598
31. Xu C., Inokuma M.S., Denham J., Golds K., Kundu P., Gold J.D., et al. Feeder-free growth of undifferentiated human embryonic stem cells. Nature 19 (2001) 971-974