Diagnosis of benzimidazole resistance in haemonchus contortus of sheep by allele specific PCR

Asian-Australasian Journal of Animal Sciences

Volumn 20, Issue 1, 2007, Pages 7-11

  Tiwari, J ^b   Kolte, A P ^a   Kumar, S ^a   Swarnkar, C P ^a   Singh, D ^a   Pathak, K M L ^b  

^a CENTRAL SHEEP AND WOOL RESEARCH INSTITUTE   (India)

^b RAJASTHAN AGRICULTURAL UNIVERSITY   (India)

Author keywords

AS PCR; Benzimidazole Resistance; Haemonchus contortus; Tubulin

Indexed keywords

HAEMONCHUS CONTORTUS; OVIS ARIES;

EID: 33847213145     PISSN: 10112367     EISSN: None     Source Type: Journal    
DOI: 10.5713/ajas.2007.7     Document Type: Article

References (42)

1. Álvarej-Sánchez, M. A., J. Pérez-García, M. A. Cruz-Rojo and F. A. Rojo-Vázquez. 2004. Real time PCR for the diagnosis of benzimidazole resistance in trichostrongylids of sheep. Vet. Parasitol. 129:291-298.
2. Annon. 2004. Annual Progress Report: All India Network Program on Gastrointestinal Parasitism. CSWRI, Avikanagar, India, p. 1-45.
3. Assan, N. and S. M. Makuza, 2005. The effect on non-genetic factors on birth weight and weaning weight in three sheep breeds of Zimbabwe. Asian-Aust. J. Anim. Sci. 18(2):151-157.
4. Beech, R. N., R. K. Prichard and M. E. Scott. 1994. Genetic variability of the beta-tubulin genes in benzimidazole susceptible and resistant strains of Haemonchus contortus. Genet. 138:103-110.
5. Coles, G. C., C. Bauer, F. H. Borgsteede, S. Geerts, T. R. Klei, M. A. Taylor and M. J. Waller. 1992. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet. Parasitol. 44:35-44.
6. Dorny, P., E. Romjali, K. Feldman, A. Barubara and V. C. Pandey. 1995. Studies on the efficacy of four anthelmintics against strongyle infections of sheep in North Sumatra, Indonesia. Asian-Aust. J. Anim. Sci. 8:347-352.
7. Elard, L., J. Cabaret and J. F. Humbert. 1999. PCR diagnosis of benzimidazole-susceptibility or -resistance in natural populations of the small ruminant parasite, Teladorsagia circumcincta. Vet. Parasitol. 80:231-237.
8. Elard, L., A. M. Comes and J. F. Humbert. 1996. Sequences of beta-tubulin cDNA from benzimidazole -susceptible and -resistant strains of Teladorsagia circumcincta, a nematode parasite of small ruminants. Mol. Biochem. Parasitol. 79:249 253.
9. Elard, L. and J. F. Humbert. 1999. Importance of the mutation of amino acid 200 of the isotype 1 beta tubulin gene in the benzimidazole resistance of the small ruminant parasite Teladorsagia circumcincta. Parasitol. Res. 85:452-456.
10. Howlader, M. M. R., S. S. Capitan, S. L. Eduarde, N. P. Roxas and C. C. Sevilla. 1996. Effect of experimental Haemonchus contenus infection on haemoglobin concentration and packed cell volume of does. Asian-Aust. J. Anim. Sci. 9:597-601.
11. Howlader, M. M. R., S. S. Capitan, S. L. Eduardo and N. P. Roxas. 1997a. Effect of experimental Haemonchus contenus infection on red blood cells and white blood cells of growing goats. Asian-Aust. J. Anim. Sci. 10:679-682.
12. Howlader, M. M. R., S. S. Capitan, S. L. Eduardo, N. P. Roxas and C. C. Sevilla. 1997b. Performance of growing goats experimentally infected with stomach worm (Haemonchus contortus). Asian-Aust. J. Anim. Sci. 10:534-539.
13. Humbert, J. F., J. Cabaret, L. Elard, V. Leignel and A. Silvestre. 2001. Molecular approaches to studying benzimidazole resistance in trichostrongylid nematode parasites of small ruminants. Vet. Parasitol. 101:405-414.
14. Jackson, F. 1993. Anthelmintic resistance- the state of play. Br. Vet. J. 149:123-138.
15. Kwa, M. S., F. N. Kooyman, J. H. Boersema and M. H. Roos. 1993. Effect of selection for benzimidazoles resistance in Haemonchus contortus on beta tubulin isotype 1 and isotype 2 genes. Biochem. Biophys. Res. Commun. 191:413-419.
16. Kwa, M. S., J. G. Veenstra and M. H. Roos. 1994. Benzimidazole resistance in Haemonchus contortus is correlated with a conserved mutation at amino acid 200 in beta-tubulin isotype 1. Mol. Biochem. Parasitol. 63:299-303.
17. Le Jambre, L. F. 1976. Egg hatch as an in vitro assay of thiabendazole resistance in nematodes. Vet. Parasitol. 2:385-391.
18. Lehrer, S., H. Davey, T. Watson and R. J. Wilkins. 1995. Sensitive PCR for detecting benzimidazole resistant sub populations of ovine nematodes in the Waikato. In Proceedings of the New Zealand Society of Animal Production, 55:209-210.
19. Mandal, A., K. P. Pant, D. R. Notter, P. K. Rout, R. Roy, N. K. Sinha and N. Sharma. 2005. Studies on inbreeding and its effects on growth and fleece traits of Muzaffarnagri sheep. Asian-Aust. J. Anim. Sci. 18(10):1363-1367.
20. Martin, P. J., N. Anderson and R. G. Jarrett. 1989. Detecting benzimidazole resistance with faecal egg count reduction tests and in vitro assays. Aust. Vet. J. 66:236-240.
21. Pape, M., J. Posedi, K. Failing, T. Schnieder and G. Von Samson-Himmelstjerna. 2003. Analysis of the beta-tubulin codon 200 genotype distribution in a benzimidazole- susceptible and resistant cyathostome population. Parasitol. 127:53-59.
22. Pierce, B. A. 2003. Genetics, A Conceptual Approach. W.H. Freeman and Company, NY, USA, pp. 671-672.
23. Roos, M. H., M. S. Kwa and W. N. Grant. 1995. New genetic and practical implications of selection for anthelmintic resistance in parasitic nematodes. Parasitol. Today. 11:148-150.
24. Silvestre, A. and J. F. Humbert. 2000. A molecular tool for species identification and benzimidazole resistance diagnosis in larval communities of small ruminant parasites. Exp. Parasitol. 95:271-276.
25. Silvestre, A. and J. F. Humbert. 2002. Diversity of benzimidazole resistance alleles in populations of small ruminants parasites. Int. J. Parasitol. 32:921-928.
26. Singh, D., R. Gulyani and V. Bhasin. 1992. Occurrence of hiabendazole resistant strains of Haemonchus contortus in sheep. Ind. Vet. Med. J. 16:139-141.
27. Singh, D., P. K. Sanyal, C. P. Swarnkar, F. A. Khan and P. S. K. Bhagwan. 1999. Influence of diet type and pre-treatment fasting on the disposition kinetics of albendazole in sheep. Vet. Res. Commun. 23:229-240.
28. Singh, D., C. P. Swarnkar and F. A. Khan. 2002. Anthelmintic resistance in gastrointestinal nematodes of livestock in India. J. Vet. Parasitol. 16:115-130.
29. Singh, D., C. P. Swarnkar, F. A. Khan, C. P. Srivastava and P. S. K. Bhagwan. 1995. Resistance to albendazole in gastrointestinal nematodes of sheep. J. Vet. Parasitol. 9:95-98.
30. Singh, D., C. P. Swarnkar, C. P. Srivastava, P. S. K. Bhagwan and U. Dimri. 1996. Haemonchus contortus resistant to rafoxanide in sheep. J. Vet. Parasitol. 10:53-56.
31. Snedecor, G. W. and W. G. Cochran. 1967. Statistical Methods. Oxfords and IBH publishing company, Culcatta, India.
32. Soulsby, E. J. L. 1965. Textbook of veterinary clinical parasitology, Vol. 1, helminths. Blackwell Scientific Publication. Great Britain, pp. 297-310.
33. Swarnkar, C. P., F. A. Khan, D. Singh and P. S. K. Bhagwan. 1999, Further studies on anthelmintic resistance in sheep at an organized farm in arid region of Rajasthan. Vet. Parasitol. 82:81-84.
34. Swarnkar, C. P., P. K. Sanyal, D. Singh, F. A. Khan and P. S. K. Bhagwan. 2001. Anthelmintic resistance on an organized sheep farm in India. Trop. Anim. Health. Prod. 33:305-312.
35. Swarnkar, C. P., D. Singh, F. A. Khan, R. Tiwari and S. C. Dubey. 2004. Prevalence of anthelmintic resistance and seasonal variation in efficacy of anthelmintics in sheep. In XV National Congress of Indian Association for the Advancement of Veterinary Parasitology, GBPUAT, Pantnagar, India, p. 72.
36. Varshney, T. R. and Y. P. Singh. 1976. A note on development of resistance of Haemonchus contortus worms against phenothiazine and thiabendazole in sheep. Indian J. Anim. Sci. 46:666-668.
37. Von Samson-Himmelstjerna, G., M. Pape, C. Von Witzendorff and T. Schnieder. 2002a. Allele specific PCR for the beta tubulin codon 200 TTC/TAC polymorphism using single adult and larval small strongyle (Cyathostominae) stages. J. Parasitol. 88:254-257.
38. Von Samson-Himmelstjerna, G., C. Von Witzendorff, G. Sievers and T. Schnieder, 2002b. Comparative use of faecal egg count reduction test, egg hatch assay and beta tubulin codon 200 genotyping in small strongyles (Cyathostominae) before and after benzimidazole treatment. Vet. Parasitol. 108:227-235.
39. Waller, P. J. 1994. The development of anthelmintic resistance in ruminant livestock. Acta Tropica 56:233-243.
40. Wheeler, I. E., S. J. Kendall, J. Butters, D. W. Hollomon and L. Hall. 1995. Using allele specific oligonucleotide probes to characterize benzimidazole resistance in Rhynchosporium secalis. Pestic. Sci. 43:201-209.
41. Winterrowd, C. A., W. E. Pomroy, N. C. Sangster, S. S. Johnson and T. G. Geary. 2003. Benzimidazole resistant beta tubulin alleles in a population of parasitic nematode (Cooperia oncophora) of cattle. Vet. Parasitol. 117:161-172.
42. Yadav, C. L., R. P. Uppal and S. Kalra. 1993. An outbreak of haemonchosis associated with anthelmintic resistance in sheep. J. Vet. Parasitol. 23:411-413.