Extraction and analysis of clonazepam and 7-aminoclonazepam in whole blood using a dual internal standard methodology

Forensic Science International

Volumn 166, Issue 2-3, 2007, Pages 209-217

  Hackett, Jeffery ^a   Elian, Albert A ^b  

^a Center for Forensic Sciences   (United States)

^b State Police Crime Laboratory   (United States)

Author keywords

Chromatography; Clonazepam; SPE; Toxicology

Indexed keywords

7 AMINOCLONAZEPAM; CLONAZEPAM; DRUG METABOLITE; NITRAZEPAM; UNCLASSIFIED DRUG;

ARTICLE; CHROMATOGRAPHY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DRUG BLOOD LEVEL; DRUG ISOLATION; EVAPORATION; GAS CHROMATOGRAPHY; HUMAN; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; PH MEASUREMENT; PRIORITY JOURNAL; SEXUAL CRIME;

EID: 33847200438     PISSN: 03790738     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.forsciint.2006.05.040     Document Type: Article

References (16)

1. Baselt R.C. Disposition of Toxic Drugs and Chemicals in Man. 7th ed. (2004), Biomedical Publications, Foster City, California, USA pp. 246-247
2. Negrusz A., Bowen A.M., Moore C.M., Dowd S.M., Strong M.J., and Janicak P.G. Deposition of 7-aminoclonazepam and clonazepam in hair following a single dose of Klonopin. J. Anal. Toxicol. 26 (2002) 471-478
3. Clonazepam: Monthly Prescribing Reference vol. 21 (2005), Prescribing Reference Inc., New York, USA p. 118
4. Le Guellec C., Gaudet M.L., and Breteau M. Improved selectivity for high-performance liquid chromatographic determination of clonazepam in plasma of epileptic patients. J. Chromatogr. B: Biomed. Sci. Appl. 719 (1998) 227-233
5. Sallustrio B.C., Kassapidis C., and Morris R.G. High-performance liquid chromatography determination of clonazepam in plasma using solid-phase extraction. Ther. Drug Monit. 16 (1994) 174-178
6. Lillsunde P., and Seppala T. Simultaneous screening and quantitative analysis of benzodiazepines by dual-channel gas chromatography using electron-capture and nitrogen-phosphorus detection. J. Chromatogr. 533 (1990) 97-110
7. Song D., Zhang S., and Kohlhof K. Quantitative determination of clonazepam in plasma by gas chromatography-negative ion chemical ionization mass spectrometry. J. Chromatogr. B: Biomed. Appl. 686 (1996) 199-204
8. Robertson M.D., and Drummer O.H. High-performance liquid chromatographic procedure for the measurement of nitrobenzodiazepines and their 7-amino metabolites in blood. J. Chromatogr. B: Biomed. Appl. 667 (1995) 179-184
9. Kratzsch C., Tenberken O., Peters F.T., Weber A.A., Kraemer T., and Maurer H.H. Screening, library-assisted identification and validated quantification of 23 benzodiazepines, flumazenil, zaleplone, zolpidem and zopiclone in plasma by liquid chromatography/mass spectrometry with atmospheric pressure chemical ionization. J. Mass Spectrom. 39 (2004) 856-872
10. Black D.A., Clark G.D., Haver V.M., Garbin J.A., and Saxon A.J. Analysis of urinary benzodiazepines using solid-phase extraction and gas chromatography-mass spectrometry. J. Anal. Toxicol. 18 (1994) 185-188
11. Negrusz A., Moore C.M., Kern J.L., Janicak P.G., Strong M.J., and Levy N.A. Quantitation of clonazepam and its major metabolite 7-aminoclonazepam in hair. J. Anal. Toxicol. 24 (2000) 614-620
12. Robertson M.D., and Drummer O.H. Postmortem distribution and redistribution of nitrobenzodiazepines in man. J Forensic Sci. 43 (1998) 9-13
13. Elian A.A. ELISA detection of clonazepam and 7-aminoclonazepam in whole blood and urine. Forensic Sci. Int. 134 (2003) 54-56
14. Clean Screen Extraction Columns Application Manual (2004), United Chemical Technologies, Bristol, PA, USA
15. Hackett J., and Elian A.A. Extraction and analysis of flunitrazepam/7-aminoflunitrazepam in blood and urine by LC-PDA and GC-MS using butyl SPE columns. Forensic Sci. Int. 157 (2006) 156-162
16. In: Moffat A.C., Osselton M.D., and Widdop B. (Eds). Clarke's Analysis of Drugs and Poisons. 3rd Ed. vol. 2 (2004), Pharmaceutical Press, London, UK pp. 829-830, 1343-1345