Preliminary study towards the use of in-situ microscopy for the online analysis of microcarrier cultivations

Engineering in Life Sciences

Volumn 7, Issue 1, 2007, Pages 91-96

  Anton, F ^a   Burzlaff, A ^a   Kasper, C ^a   Bruckerhoff T ^a   Scheper, T ^a  

^a LEIBNIZ UNIVERSITY HANNOVER   (Germany)

Author keywords

Biotechnology; Cell cultures; Cell growth; Microscopy

Indexed keywords

CELL CULTURE; CELL GROWTH; DENSITY (OPTICAL); IMAGE ANALYSIS; MICROSCOPIC EXAMINATION;

CELL DENSITY; GREYSCALE PATTERN; MICROCARRIERS;

BIOTECHNOLOGY;

BIOTECHNOLOGY; CELL CULTURE; CELL GROWTH; DENSITY (OPTICAL); IMAGE ANALYSIS; MICROSCOPIC EXAMINATION;

ALGORITHM; BIOTECHNOLOGY; HISTOGRAM; IMAGE ANALYSIS; IMAGING METHOD; MICROSCOPY;

ALGORITHM; ANIMAL CELL; ARTICLE; AUTOMATION; CALIBRATION; CELL COUNT; CELL CULTURE; CELL DENSITY; CONTROLLED STUDY; FIBROBLAST; HISTOGRAM; IMAGE ANALYSIS; IN SITU MICROSCOPY; MICROCARRIER CULTURE; MICROSCOPY; MOUSE; NON INVASIVE MEASUREMENT; NONHUMAN; ONLINE ANALYSIS; OPTICAL DENSITY;

EID: 33847159854     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.200620172     Document Type: Article

References (33)

1. B. Griffiths, Alternative strategies to the scale-up of animal cells, Ann. N. Y. Acad. Sci. 1992, 665, 364-370.
2. B. Griffiths, Scale-up of suspension and anchorage-dependent animal cells, Mol. Biotech. 2001, 17 (3), 225-238.
3. J. B. Griffiths, D. R. Cameron, D. Looby, A comparison of unit process systems for anchorage dependent cells, Dev. Biol. Stand. 1987, 66, 331-338.
4. J. Y. Liu, J. Hafner, G. Dragieva, G. Burg, Bioreactor microcarrier cell culture system (Bio-MCCS) for large-scale production of autologous melanocytes, Cell Transplant. 2004, 13 (7-8), 809-816.
5. B. Montagnon, J.C. Vincent-Falquet, B. Fanget, Thousand litre scale microcarrier culture of Vero cells for killed polio virus vaccine: Promising results, Dev. Biol. Stand. 1983, 55, 3-42.
6. B. Meignier, Cell culture on beads used for the industrial production of foot and mouth disease virus, Dev. Biol. Stand. 1979, 42, 141-145.
7. A. L. van Wezel, C.A.M. van der Velden-de Groot, Large scale cultivation of animal cells in microcarrier culture, Process Biochem. 1978, 13, 6-8.
8. D. H. Lewis, S. A. Volkers, Use of a new bead microcarrier for the culture of anchorage dependent cells in pseudo suspension, Dev. Biol. Stand. 1979, 42, 147-151.
9. K. Nilsson, Microcarrier cell culture, Biotechnol. Genet. Eng. Rev. 1988, 6, 403-439.
10. M. Reiter, F. Weigang, W. Ernst, H. W. Katinger, High density microcarrier culture with a new device which allows oxygenation and perfusion of microcarrier cultures, Cytotechnology 1990, 3 (1), 39-42.
11. N. A. Bleckwenn, H. Golding, W. E. Bentley, J. Shiloach, Production of recombinant proteins by vaccinia virus in a microcarrier based mammalian cell perfusion bioreactor, Biotechnol. Bioeng. 2005, 90 (6), 663-674.
12. D. J. Giard, W. G. Thilly, D. I. Wang, D. W. Levine, Virus production with a newly developed microcarrier system, Appl. Environ. Microbiol. 1977, 34 (6), 668-672.
13. J. B. Griffiths, I. D. McEntee, A. Electricwala, A. Atkinson, P. M. Sutton, S. Naish et al., The production and properties of a tissue plasminogen activator from normal epithelial cells grown in microcarrier culture, Dev. Biol. Stand. 1985, 60, 439-446.
14. J. M. Sautier, J. R. Nefussi, N. Forest, Mineralization and bone formation on microcarrier beads with isolated rat calvaria cell population, Calcif. Tissue Int. 1992, 50 (6), 527-532.
15. M. Shima, Y. Seino, H. Tanaka, H. Kurose, M. Ishida, H. Yabuuchi et al., Microcarriers facilitate mineralization in MC3T3-E1 cells, Calcif. Tissue Int. 1988, 43 (1), 19-25.
16. A. Shnyra, A. Bocharov, N. Bochkova, V. Spirov, Large-scale production and cultivation of hepatocytes on Biosilon microcarriers, Artif. Organs 1990, 14 (6), 421-428.
17. M. Spearman, J. Rodriguez, N. Huzel, M. Butler, Production and glycosylation of recombinant beta-interferon in suspension and cytopore microcarrier cultures of CHO cells, Biotechnol. Progr. 2005, 21 (1), 31-39.
18. J. S. Tang, C. F. Chao, M. K. Au, Growth and metabolism of cultured bone cells using microcarrier and monolayer techniques, Clin. Orthop. Relat. Res. 1994, (300), 254-258.
19. D. Wang, C. Xiao, Z. Chen, Z. Huang, Studies on high-density cultivation of Vero cells with biosilon solid microcarrier, Chin. J. Biotechnol. 1996, 12 (2), 119-129.
20. P. van Hemert, D. G. Kilburn, A. L. van Wezel, Homogeneous cultivation of animal cells for the production of virus and virus products, Biotechnol. Bioeng. 1969, 11 (5), 875-885.
21. B. Meignier, H. Mougeot, H. Favre, Foot and mouth disease virus production on microcarrier-grown cells, Dev. Biol. Stand. 1980, 46, 249-256.
22. J. M. Clark, M. D. Hirtenstein, High yield culture of human fibroblasts on microcarriers: a first step in production of fibroblast-derived interferon (human beta interferon), J. Interferon Res. 1981, 1 (3), 391-400.
23. J. Clark, H. Hirstenstein, C. Gebb, Critical parameters in the microcarrier culture of animal cells, Dev. Biol. Stand. 1980, 46, 117-124.
24. P. Ducommun, I. Bolzonella, M. Rhiel, P. Pugeaud, U. von Stockar, I. W. Marison, On-line determination of animal cell concentration, Biotechnol. Bioeng. 2001, 72 (5), 515-522.
25. K. K. Sanford, W. R. Earle, V. J. Evans, H. K. Waltz, J. E. Shannon, The measurement of proliferation in tissue cultures by enumeration of cell nuclei, J. Nat. Cancer Inst. 1951, 11 (4), 773-795.
26. M. Absher, Hemocytometer counting, in Tissue Culture: Methods and Applications (Ed: P. F. Kruse, J. R. Patterson), Academic Press, London 1973, 395-397.
27. H. Suhr, G. Wehnert, K. Schneider, C. Bittner, T. Scholz, P. Geissler et al., In situ microscopy for on-line characterization of cell populations in bioreactors, including cell-concentration measurements by depth from focus, Biotechnol. Bioeng. 1995, 47 (1), 106-116.
28. J.-G. Frerichs, Entwicklung eines In-situ Mikroskops zur bildgestützten Online-Überwachung von Bioprozessen, PD Thesis, Universitát Hannover 2000.
29. C. Bittner, G. Wehnert, T. Scheper, In situ microscopy for on-line determination of biomass, Biotechnol. Bioeng. 1998, 60 (1), 24-35.
30. K. Joeris, J.-G. Frerichs, K. Konstantinov, T. Scheper, In-situ microscopy: Online process monitoring of mammalian cell cultures, Cytotechnology 2002, 38 (1-2), 129-134.
31. R. Ulber, J.-G. Frerichs, S. Beutel, Optical sensor systems for bioprocess monitoring, Anal Bioanal. Chem. 2003, 376 (3), 342-348.
32. V. Camisard, J. Brienne, H. Baussart, J. Hammann, H. Suhr, Inline characterization of cell concentration and cell volume in agitated bioreactors using in situ microscopy: Application to volume variation induced by osmotic stress, Biotechnol. Bioeng. 2002, 78 (1), 73-80.
33. J. Guez, J. Cassar, F. Wartelle, P. Dhulster, H. Suhr, Real time in situ microscopy for animal cell-concentration monitoring during high density culture in bioreactor, J. Biotechnol. 2004, 111 (3), 335-343.