A new quantitative real-time reverse transcriptase PCR assay and melting curve analysis for detection and genotyping of Ljungan virus strains

Journal of Virological Methods

Volumn 141, Issue 1, 2007, Pages 71-77

  Donoso Mantke, Oliver ^a   Kallies, René ^a   Niklasson, Bo ^b,c   Nitsche, Andreas ^a   Niedrig, Matthias ^a  

^a ROBERT KOCH INSTITUTE   (Germany)

^b Apodemus AB   (Sweden)

^c UPPSALA UNIVERSITY   (Sweden)

Author keywords

Aetiological diagnostic; Ljungan virus; Melting curve analysis; Minor groove binder probes; Real time RT PCR; Viral zoonoses

Indexed keywords

5' UNTRANSLATED REGION; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DIABETES MELLITUS; GENOTYPE; HUMAN; HUMAN CELL; HUMAN TISSUE; LJUNGAN VIRUS; MOUSE; MYOCARDITIS; NEUROLOGIC DISEASE; NONHUMAN; NUCLEOTIDE SEQUENCE; PERINATAL MORBIDITY; PICORNAVIRUS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRUS CULTURE; VIRUS DETECTION; VIRUS LOAD; VIRUS STRAIN;

ANIMALS; DNA PROBES; GENOTYPE; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PARECHOVIRUS; PICORNAVIRIDAE INFECTIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TRANSITION TEMPERATURE;

LJUNGAN VIRUS; MURIDAE; PICORNAVIRIDAE; RODENTIA;

EID: 33847083586     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2006.11.029     Document Type: Article

References (14)

1. Chomczynski P., and Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 (1987) 156-159
2. Hughes A.L. Phylogeny of the Picornaviridae and differential evolutionary divergence of picornavirus proteins. Infect. Genet. Evol. 4 (2004) 143-152
3. Johansson S., Niklasson B., Maizel J., Gorbalenya A.E., and Lindberg A.M. Molecular analysis of three Ljungan virus isolates reveals a new, close-to-root lineage of the Picornaviridae with a cluster of two unrelated 2A proteins. J. Virol. 76 (2002) 8920-8930
4. Johansson E.S., Niklasson B., Tesh R.B., Shafren D.R., Travassos da Rosa A.P.A., and Lindberg A.M. Molecular characterization of M1146, an American isolate of Ljungan virus (LV) reveals the presence of a new LV genotype. J. Gen. Virol. 84 (2003) 837-844
5. Johansson E.S., Ekström J.-O., Shafren D.R., Frisk G., Hyypiä T., Edman K., and Lindberg A.M. Cell culture propagation and biochemical analysis of the Ljungan virus prototype strain. Biochem. Biophys. Res. Commun. 317 (2004) 1023-1029
6. Kutyavin I.V., Afonina I.A., Mills A., Gorn V.V., Lukthanov E.A., Belousov E.S., Singer M.J., Walburger D.K., Lokhov S.G., Gall A.A., Dempcy R., Reed M.W., Meyer R.B., and Hedgpeth J. 3′-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucl. Acids Res. 28 (2000) 655-661
7. Lindberg A.M., and Johansson S. Phylogenetic analysis of Ljungan virus and A-2 plaque virus, new members of the Picornaviridae. Virus Res. 85 (2002) 61-70
8. Niklasson B., Hörnfeldt B., Lundkvist A., Björsten S., and LeDuc J. Temporal dynamics of Puumala virus antibody prevalence in voles and of nephropathia epidemica incidence in humans. Am. J. Trop. Med. Hyg. 53 (1995) 134-140
9. Niklasson B., Hörnfeldt B., and Lundman B. Could myocarditis, insulin dependent diabetes mellitus, and Guillain-Barré syndrome be caused by one or more infectious agents carried by rodents?. Emerg. Infect. Dis. 4 (1998) 187-193
10. Niklasson B., Kinnunen L., Hörnfeldt B., Hörling J., Benemar C., Hedlund K.O., Matskova L., Hyypiä T., and Winberg G. A new picornavirus isolated from bank voles (Clethrionomys glareolus). Virology 255 (1999) 86-93
11. Niklasson B., Samsioe A., Blixt M., Sandler S., Sjöholm A., Lagerquist E., Lernmark A., and Klitz W. Prenatal viral exposure followed by adult stress produces glucose intolerance in a mouse model. Diabetologia 49 (2006) 2192-2199
12. Racaniello V.R. Picornaviridae: the viruses and their replication. In: Knipe D.M., Howley P.M., Griffin D.E., Lamb R.A., Matrin M.A., Riozman B., and Straus S.E. (Eds). Fields Virology. 4th ed. (2001), Williams & Wilkins, Philadelphia 685-722
13. Samsioe A., Feinstein R., Saade G., Sjöholm A., Hörnfeldt B., Fundele R., Klitz W., and Niklasson B. Intrauterine death, fetal malformation, and delayed pregnancy in Ljungan virus-infected mice. Birth Defects Res. B: Dev. Reprod. Toxicol. 7 (2006) (Epub ahead of print)
14. Wesslén L., Påhlson C., Friman G., Fohlman J., Lindquist O., and Johansson C. Myocarditis caused by Chlamydia pneumoniae (TWAR) and sudden unexpected death in a Swedish elite orienteer. Lancet 340 (1992) 427-428