Icariine stimulates proliferation and differentiation of human osteoblasts by increasing production of bone morphogenetic protein 2

Chinese Medical Journal

Volumn 120, Issue 3, 2007, Pages 204-210

  Yin, Xiao Xue ^a   Chen, Zhong Qiang ^a   Liu, Zhong Jun ^a   Ma, Qing Jun ^a   Dang, Geng Ting ^a  

^a PEKING UNIVERSITY THIRD HOSPITAL   (China)

Author keywords

Bone morphogenetic protein; Cell differentiation; Cell proliferation; Icariine; Osteoblasts

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ALKALINE PHOSPHATASE; ICARIINE; MESSENGER RNA; PLANT MEDICINAL PRODUCT; RECOMBINANT BONE MORPHOGENETIC PROTEIN 2; UNCLASSIFIED DRUG;

ADULT; ARTICLE; BONE MARROW CELL; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STIMULATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG MECHANISM; ENZYME ACTIVITY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; IN VITRO STUDY; MESENCHYME CELL; OSTEOBLAST; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SYNTHESIS;

EID: 33847080418     PISSN: 03666999     EISSN: None     Source Type: Journal    
DOI: 10.1097/00029330-200702010-00006     Document Type: Article

References (23)

1. Cummings SR, Melton LJ. Epidemiology and outcomes of osteoporotic fractures. Lancet 2002; 359: 1761-1767.
2. Ma Z, Wang R, Qiu M. Study of morphologic effects of 4 Chinese herbs by bone histomorphometry in ovariectomized rats. Chin J Obstet Gynecol (Chin) 1999; 34: 82-85.
3. Wang B, Quan J, Guo S. Effects of epimedium on the expression of interleukin-6 messenger ribonucleic acid in bone of ovariectomized rat. Chin J Obstet Gynecol (Chin) 2000; 35: 724-726.
4. Yu S, Chen K, Li S, Zhang K. In vitro and in vivo studies of the effect of a Chinese herb medicine on osteoclastic bone resorption. Chin J Dent Res (Chin) 1999; 2: 7-11.
5. Li J, Yu S, Li T, Pang S. In vitro study of the effects of Epimedium on osteoclastic bone resorption in various oral mineralized tissues. Chin J Stomatol (Chin) 2002; 37: 391-394.
6. Xue Y, Wang P, Qi QH, Ni H, Ma XL, Guo SF, et al. The experimental study of the effects of icariine on increasing Smad4mRNA level in MC3T3-E1 cell in vitro. Chin J Orthop (Chin) 2005; 25: 119-123.
7. Meng FH, Li YB, Xiong ZL, Jiang ZM, Li FM. Osteoblastic proliferative activity of Epimedium brevicomum Maxim. Phytomedicine 2005; 12:189-93.
8. Yin XX, Chen ZQ, Guo ZQ, Dang GT, Ma QJ, Wang SH. Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene. Biochem Biophy Res Commun 2004; 315: 643-651.
9. Yin XX, Chen ZQ, Guo ZQ, Ma QJ, Dang GT. Inducting human marrow mesenchymal stem cell into osteoblast directionally and the identification of their osteogenesis characteristics. Chin J Reparat Reconstruct Surg (Chin) 2004; 18: 98-101.
10. Yin XX, Chen ZQ, Dang GT, Wang ZG, Zhang HZ. Induction and differentiation of human marrow mesenchymal stem cells into osteoblasts directionally and its features of osteogenesis in vivo. Chin J Clin Rehabilit (Chin) 2005; 9: 72-73.
11. Huang HJ, Ping FY. Synthesis and Evaluation of Human Bone Morphogenetic Protein 2 cDNA probe. Zhejiang J Trauma Surg (Chin) 2004; 9: 9-11.
12. Ikegame M, Ishibashi O, Yoshizawa T, Shimomura J, Komori T, Ozawa H, et al. Tensile stress induces bone morphogenetic protein 4 in preosteoblastic and cells, fibroblastic, which later differentiate into osteoblasts leading to osteogenesis in the mouse calvariae in organ culture. J Bone Miner Res 2001; 16: 24-32.
13. Cai ML, Ji H, Li P, Wang MQ. Effects of five flavonoids isolated from Epimedium pubescens on osteoblasts in vitro. Chin J Nat Med (Chin) 2004; 2: 235-238.
14. Ji H, Liu K, Li SP, Gong XJ. Effect of HEF on Osteoporosis Induced by Retinoic Acid in Male Rats. J Chin Pharm Univ (Chin) b2000; 31: 222-225.
15. Ji H, Liu K, Gong XJ, Li SP, Zhang MT. Effects of Epimidium koreanum flavoids on osteoporosis in overiectomized rats. Chin J Osteoporos (Chin) 2001; 7: 4-48.
16. Li Y, Ji H, Li P, Xie L. effetcs of Epimedium pubescens Flavonoids on osteoblasts in vitro. J Chin Pharm University (Chin) 2002; 33: 48-50.
17. Stein GS, Lian JB. Molecular mechanisms mediating proliferation/ differention interrelationships during progressive development of the osteoblast phenotype. Endocr Rev 1993; 14: 424-422.
18. Wozney JM, Rosen V, Celeste AJ, Mitsock LM, Whitters MJ, Kriz RW, et al. Novel regulators of bone formation: molecular clones and activities. Science 1988; 242: 1528-1533.
19. Wang EA, Rosen V, Cordes P, Hewick RM, Kriz MJ, Luxenberg DP, et al. Purification and characterization of other distinct bone-inducing factors. Proc Natl Acad Sci USA 1988; 85: 9484-9488.
20. Storm EE, Hunyh TV, Copeland NG, Jenkins NA, Kingsley DM, Lee SJ. Limb alterations in brachypodism mice due to mutations in a new member of the TGF-beta superfamily. Nature 1994; 368: 639-643.
21. Sampath TK, Maliakal JC, Hauschka PV, Jones WK, Sasak H, Tucker RF, et al. Recombinant human osteogenic protein-1 (hOP-1) induces new bone formation in vivo with a specific activity comparable with natural bovine osteogenic protein and stimulates osteoblast proliferation and differentiation in vitro. J Biol Chem 1992; 267: 20352-20362.
22. Wang EA, Rosen V, D'Alessandro JS, Bauduy M, Cordes P, Harada T, et al. Recombinant human bone morphogenetic protein induces bone formation. Proc Natl Acad Sci U S A 1990; 87: 2220-2224.
23. Lecanda F, Avioli LV, Cheng SL. Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. J Cell Biochem 1997; 67: 386-396.