Direct analysis of 15N-label in amino and amide groups of glutamine and asparagine

Journal of Mass Spectrometry

Volumn 42, Issue 2, 2007, Pages 161-170

  Scharff Poulsen, Anne Marie ^a   Schou, Christian ^b   Egsgaard, Helge ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b STATENS SERUM INSTITUT   (Denmark)

Author keywords

Amino acids; Electrospray mass spectrometry; Ion structures; Ion pair chromatography; Isotope labeling

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; DERIVATIVES; IONS; ISOTOPES; MASS SPECTROMETRY; NONLINEAR SYSTEMS;

ELECTROSPRAY MASS SPECTROMETRY; ION PAIR CHROMATOGRAPHY; ION STRUCTURES; ISOTOPE LABELING;

AMINO ACIDS;

AMIDE; AMINO ACID; ASPARAGINE; GLUTAMINE; NITROGEN;

ANALYTIC METHOD; ARTICLE; CHEMICAL ANALYSIS; CHEMICAL LABELING; FRAGMENTATION REACTION; ION PAIR CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN STRUCTURE; ELECTROSPRAY MASS SPECTROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; METHODOLOGY;

ASPARAGINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLUTAMINE; ISOTOPE LABELING; NITROGEN ISOTOPES; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 33847074657     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1134     Document Type: Article

References (23)

1. Scharff AM, Egsgaard H, Hansen PE, Rosendahl L. Exploring symbiotic nitrogen fixation and assimilation in pea root nodules by in vivo N-15 nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry. Plant Physiol. 2003; 131: 367.
2. Buchanan BB, Gruissem W, Jones RL. In Biochemistry and Molecular Biology of Plants, 5th edn. John Wiley and Sons: Somerset, NJ, 2005; 358.
3. 15N glutamine enrichment with tertiary butyldimethylsilyl derivatives. Biol. Mass Spectrom. 1994; 23: 682.
4. 14N position-specific isotopic analyses of polynitrogenous amino acids. Anal. Chem. 2005; 77: 1013.
5. Petritis KN, Chaimbault P, Elfakir C, Dreux M. Ion-pair reversed-phase liquid chromatography for determination of polar underivatized amino acids using perfluorinated carboxylic acids as ion pairing agent. J. Chromatogr., A 1999; 833: 147.
6. Kwon JY, Moini M. Analysis of underivatized amino acid mixtures using high performance liquid chromatography/dual oscillating nebulizer atmospheric pressure microwave induced plasma ionisation-mass spectrometry. J. Am. Soc. Mass Spectrom. 2001; 12: 117.
7. Petritis KN, Chaimbault P, Elfakir C, Dreux M. Parameter optimization for the analysis of underivatized protein amino acids by liquid chromatography and ionspray tandem mass spectrometry. J. Chromatogr., A 2000; 896: 253.
8. Piraud M, Vianey-Saban C, Petritis K, Elfakir C, Steghens JP, Morla A, Bouchu D. ESI-MS/MS analysis of underivatised amino acids: a new tool for the diagnosis of inherited disorders of amino acid metabolism. Fragmentation study of 79 molecules of biological interest in positive and negative ionisation mode. Rapid Commun. Mass Spectrom. 2003; 17: 1297.
9. Dookeran NN, Yalcin T, Harrison AG. Fragmentation reactions of protonated α-amino acids. J. Mass Spectrom. 1996; 31: 500.
10. Rogalewicz F, Hoppilliard Y, Ohanessian G. Fragmentation mechanisms of α-amino acids protonated under electrospray ionization: a collision activation and ab initio theoretical study. Int. J. Mass Spectrom. 2000; 195/196: 565.
11. Tsang CW, Harrison AG. Chemical ionization of amino acids. J. Am. Chem. Soc. 1976; 98: 1301.
12. Bouchoux G, Bourcier S, Hoppilliard Y, Mauriac C. Leucine and isoleucine in chemical ionization and plasma desorption mass spectrometry: A comparative study. Org. Mass Spectrom. 1993; 28: 1064.
13. Beranová vS, Cai J, Wesdemiotis C. Unimolecular chemistry of protonated glycine and its neutralized form in the gas phase. J. Am. Chem. Soc. 1995; 117: 9492.
14. Kulik W, Heerma W. A study of the positive and negative ion fast atom bombardment mass spectra of α-amino acids. Biomed. Environ. Mass Spectrom. 1988; 15: 419.
15. Bouchonnet S, Denhez JP, Hoppilliard Y, Mauriac C. Is plasma desorption mass spectrometry useful for small-molecule analysis? Fragmentation of natural α-amino acids. Anal. Chem. 1992; 64: 743.
16. Harrison AG. Ion chemistry of protonated glutamic acid derivatives. Int. J. Mass Spectrom. 2001; 210/211: 361.
17. Soga T, Kabazu Y, Robert M, Tomita M, Nishioka T. Qualitative and quantitative analysis of amino acids by capillary electrophoresis-electrospray ionization-tandem mass spectrometry. Electrophoresis 2004; 25: 1964.
18. Lioe H, O'Hair RAJ, Reid GE. Gas-phase reactions of protonated tryptophan. J. Am. Soc. Mass Spectrom. 2004; 15: 65.
19. Aribi Hel, Orlova G, Hopkinson AC, Siu KWM. Gas-phase fragmentation reactions of protonated aromatic amino acids: Concomitant and consecutive neutral eliminations and radical cation formations. J. Phys. Chem. A 2004; 108: 3844.
20. Campbell IM. Incorporation and dilution values - Their calculation in mass spectrally assayed stable isotope labeling experiments. Bioorg. Chem. 1974; 3: 386.
21. Harrison AG, Ichikawa H. Site of protonation in the chemical ionization mass spectra of olefinic methyl ester. Org. Mass Spectrom. 1980; 5: 245.
22. Ellenberger MR, Eades RA, Thomsen MW, Farneth WE, Dixon DA. Proton affinities of ethylidenimine and vinylamine. J. Am. Chem. Soc. 1979; 101: 7151.
23. Eades RA, Weil DA, Ellenberger MR, Farneth WE, Dixon DA, Douglass CH Jr. Electronic structure of vinylamine. Proton affinity and conformational analysis. J. Am. Chem. Soc. 1981; 103: 5372.