HuR binds to a single site on the C/EBPβ mRNA of 3T3-L1 adipocytes

Biochemical and Biophysical Research Communications

Volumn 355, Issue 1, 2007, Pages 217-220

  Jones, Heath ^a   Carver, Melinda ^a   Pekala, Phillip H ^a  

^a EAST CAROLINA UNIVERSITY   (United States)

Author keywords

3T3 L1; Adipocyte; CCAAT enhancer binding protein ; Differentiation; ELAV; HuR

Indexed keywords

CCAAT ENHANCER BINDING PROTEIN BETA; HUR PROTEIN; MESSENGER RNA;

ADIPOCYTE; ANIMAL CELL; ARTICLE; BINDING SITE; CELL CYCLE; CELL DIFFERENTIATION; CELL STRAIN 3T3; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROADIPOCYTE;

3T3 CELLS; 5' UNTRANSLATED REGIONS; ADIPOCYTES; ANIMALS; ANTIGENS, SURFACE; BINDING SITES; CCAAT-ENHANCER-BINDING PROTEIN-BETA; CELL DIFFERENTIATION; CELL NUCLEUS; CYTOSOL; DNA PRIMERS; LIGANDS; MICE; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; RNA, MESSENGER; RNA-BINDING PROTEINS;

EID: 33847040347     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.01.136     Document Type: Article

References (12)

1. Otto T.C., and Lane M.D. Adipose development: from stem cell to adipocyte. Crit. Rev. Biochem. Mol. Biol. 40 (2005) 229-242
2. Zuo Y., Qiang L., and Farmer S.R. Activation of CCAAT/enhancer binding protein (C/EBP) α expression by C/EBPβ during adipogenesis requires a peroxisome proliferator activated receptor associated repression of HDAC1 a the C/ebpα gene promoter. J. Biol. Chem. 281 (2006) 7960-7967
3. Tang Q.-Q., Otto T.C., and Lane M.D. CCAAT/enhancer-binding protein beta is required for mitotic clonal expansion during adipogenesis. Proc. Natl. Acad. Sci. USA 100 (2003) 850855
4. Zhang J.W., Tang Q.-Q., Vinson C., and Lane M.D. Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytes. Proc. Natl. Acad. Sci. USA 101 (2004) 43-47
5. Cherry J., Karschner V., Jones H., and Pekala P.H. HuR, an RNA binding protein involved in cellular differentiation. In Vivo 20 (2006) 17-24
6. Myer V.E., Fan X.C., and Steitz J.A. Identification of HuR as a protein implicated in AUUUA-mediated mRNA decay. EMBO 16 (1997) 2130-2139
7. Ma W.J., Cheng S., Campbell C., Wright A., and Furneaux H. Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein. J. Biol. Chem. 271 (1996) 8144-8151
8. Peng S.S., Chen C.Y., Xu N., and Shyu A.B. RNA stabilization by the AU-rich element binding protein, HuR, an ELAV protein. EMBO J. 17 (1998) 3461-3470
9. Gantt K., Cherry J., Tenney T., Karschner V., and Pekala P.H. An early event in adipogenesis, the nuclear selection of C/EBPβ mRNA by HuR and it's translocation to the cytosol. J. Biol. Chem. 280 (2005) 24768-24774
10. Eddy S.R., and Durbin R. RNA sequence analysis using covariance models. Nucleic Acids Res. 22 (1994) 2079-2088
11. Prechtel A.T., Chemnitz J., Schirmer S., Ehlers C., Langbein-Detsch I., Stulke J., Dabauvalle M.D., Kehlenbach R.H., and Hauber H. Expression of CD83 is regulated by HuR via a novel cis-active coding region RNA element. J. Biol. Chem. 281 (2006) 10912-10925
12. Jain R.G., Andrews L.G., McGowan K.M., Pekala P.H., and Keene J.D. Ectopic expression of Hel-N1, an RNA-binding protein, increases glucose transporter (GLUT1) expression in 3T3-L1 adipocytes. Mol. Cell. Biol. 7 (1997) 954-962