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Volumn 48, Issue 12, 2007, Pages 2189-2192

Metalated peptide fibers derived from a natural metal-binding peptide motif

Author keywords

[No Author keywords available]

Indexed keywords

COPPER; MANGANESE; METAL COMPLEX; METAL ION; METALLOPROTEIN; PEPTIDE DERIVATIVE; PRION PROTEIN; SILVER;

EID: 33847037474     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tetlet.2007.01.089     Document Type: Article
Times cited : (16)

References (41)
  • 16
    • 33847065244 scopus 로고    scopus 로고
    • note
    • 2O for 10 h in 50% aqueous methanol at room temperature and then the solvent was evaporated under reduced pressure. The residues were washed with methanol and dried under vacuum. Metalated peptide conjugates 2-4 were satisfactorily characterized through MALDI ToF and ESI mass spectral analyses.
  • 19
    • 33847008454 scopus 로고    scopus 로고
    • note
    • EPR spectra were recorded on a Varian spectrometer at X-band frequency and the magnetic field strength was calibrated using DPPH (g = 2.0036). The preformed 1:2 (conjugate/copper and manganese) copper and manganese conjugates 2 and 4 were dissolved in 50% aqueous methanol and EPR spectra were recorded at liquid nitrogen temperature. For pH dependent EPR studies of conjugate 2 (1 mM), the pH was maintained by addition of 10 mM NaOH or HCl. Methanol was added to the aqueous solution to increase the resolution and to avoid aggregation.
  • 32
    • 33847056413 scopus 로고    scopus 로고
    • note
    • CD spectra were recorded at 25 °C under a constant flow of nitrogen on a JASCO-810 spectropolarimeter, which was calibrated with an aqueous solution of ammonium d-camphor-10-sulfonate. Experimental measurements were carried out in water (sample concentration was fixed at 0.33 mM, pH 7.5), using a 1 mm path length cuvette between 190 and 400 nm. The spectrum represents an average of 5-8 scans and the CD intensities are expressed in mdeg.
  • 39
    • 33847071812 scopus 로고    scopus 로고
    • note
    • ® software, while the data analysis was done with the aid of visual scanning probe microscopy. Conjugates 2-4 (0.33 mM) were incubated in pure water and micrographs were recorded for selected incubation periods. 10 μL of an aqueous solution of each conjugate was transferred onto a freshly cleaved mica surface and uniformly spread with the aid of a spin coater operating at 200-500 rpm (PRS-4000). The sample-coated mica was dried for 30 min at room temperature, followed by AFM imaging.
  • 41
    • 33847057479 scopus 로고    scopus 로고
    • note
    • TGA analysis. Conjugates 1-4 were incubated at 37 °C for 15 days after which the thermal stability of the metallic peptide fibers was determined by thermogravimetric analysis. Thermogravimetric analysis curves were obtained on a Perkin-Elmer Pyris6 thermogravimetry analyzer under zero-grade dry nitrogen atmosphere.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.