Estradiol induces expression of 5-hydroxytryptamine (5-HT) 4, 5-HT5, and 5-HT6 receptor messenger ribonucleic acid in rat anterior pituitary cell aggregates and allows prolactin release via the 5-HT4 receptor

Endocrinology

Volumn 148, Issue 3, 2007, Pages 1384-1395

  Papageorgiou, A ^a   Denef, Carl ^a  

^a UNIVERSITY OF LEUVEN   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

1 METHYL 3 INDOLECARBOXYLIC ACID [1 [2 (METHYLSULFONYLAMINO)ETHYL] 4 PIPERIDINYLMETHYL] ESTER; 1,2,3,5 TETRAHYDRO 5 METHYL 1 (3 PYRIDYLCARBAMOYL)PYRROLO[2,3 F]INDOLE; 2,3,6,7 TETRAHYDRO 1' METHYL 5 [[2' METHYL 4' (5 METHYL 1,2,4 OXADIAZOL 3 YL) 4 BIPHENYLYL]CARBONYL]SPIRO[FURO[2,3 F]INDOLE 3,4' PIPERIDINE]; 3 [2 [2 (4 METHYL 1 PIPERIDINYL)ETHYL] 1 PYRROLIDINESULFONYL]PHENOL; 3 [4 (3 CHLOROPHENYL) 1 PIPERAZINYL] 1,1 DIPHENYL 2 PROPANOL; 8 [4 (5 AMINO 2,4 DIMETHOXYBENZOYL)BUTYL] 1,3,8 TRIAZASPIRO[4.5]DECANE 2,4 DIONE; ALPHA METHYLSEROTONIN; CISAPRIDE; CITALOPRAM; CYCLIC AMP; ESTRADIOL; KETANSERIN; MESSENGER RNA; METHYSERGIDE; METITEPINE; N [2 [4 (2 METHOXYPHENYL) 1 PIPERAZINYL]ETHYL] N (2 PYRIDYL)CYCLOHEXANECARBOXAMIDE; ONDANSETRON; PROLACTIN; SEROTONIN 1 ANTAGONIST; SEROTONIN 2 AGONIST; SEROTONIN 2 ANTAGONIST; SEROTONIN 3 ANTAGONIST; SEROTONIN 4 AGONIST; SEROTONIN 4 ANTAGONIST; SEROTONIN 4 RECEPTOR; SEROTONIN 5 RECEPTOR; SEROTONIN 6 ANTAGONIST; SEROTONIN 6 RECEPTOR; SEROTONIN TRANSPORTER; UNCLASSIFIED DRUG; UNINDEXED DRUG; VESICULAR MONOAMINE TRANSPORTER;

ADENOHYPOPHYSIS; ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DOSE RESPONSE; DRUG EFFECT; DRUG POTENTIATION; HORMONAL REGULATION; MALE; NEUROMODULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHARMACOLOGICAL BLOCKING; PRIORITY JOURNAL; PROLACTIN RELEASE; RAT; RECEPTOR BLOCKING; RECEPTOR UPREGULATION; SEROTONINERGIC TRANSMISSION; UPREGULATION;

ANIMALS; CELLS, CULTURED; CYCLIC AMP; ESTRADIOL; GENE EXPRESSION; MALE; PERTUSSIS TOXIN; PITUITARY GLAND, ANTERIOR; PROLACTIN; RATS; RATS, WISTAR; RECEPTORS, SEROTONIN; RECEPTORS, SEROTONIN, 5-HT4; RNA, MESSENGER; SEROTONIN AGONISTS; SEROTONIN ANTAGONISTS;

EID: 33847009911     PISSN: 00137227     EISSN: 00137227     Source Type: Journal    
DOI: 10.1210/en.2006-1198     Document Type: Article

References (106)

1. Hardman JG, Limbird LE 2001 Goodman and Gilman's the pharmacological basis of therapeutics. New York: McGraw-Hill
2. Azmitia EC 2001 Modern views on an ancient chemical: serotonin effects on cell proliferation, maturation, and apoptosis. Brain Res Bull 56:413-424
3. Matsuda M, Imaoka T, Vomachka AJ, Gudelsky GA, Hou Z, Mistry M, Bailey JP, Nieport KM, Walther DJ, Bader M, Horseman ND 2004 Serotonin regulates mammary gland development via an autocrine-paracrine loop. Dev Cell 6:193-203
4. Slominski A, Wortsman J, Tobin DJ 2005 The cutaneous serotoninergic/melatoninergic system: securing a place under the sun. FASEB J 19:176-194
5. Hoyer D, Hannon JP, Martin GR 2002 Molecular, pharmacological and functional diversity of 5-HT receptors. Pharmacol Biochem Behav 71:533-554
6. Walther DJ, Peter JU, Winter S, Holtje M, Paulmann N, Grohmann M, Vowinckel J, Alamo-Bethencourt V, Wilhelm CS, Ahnert-Hilger G, Bader M 2003 Serotonylation of small GTPases is a signal transduction pathway that triggers platelet α-granule release. Cell 115:851-862
7. Van de Kar LD, Bethea CL 1982 Pharmacological evidence that serotonergic stimulation of prolactin secretion is mediated via the dorsal raphe nucleus. Neuroendocrinology 35:225-230
8. Fessler RG, Deyo SN, Meltzer HY, Miller RJ 1984 Evidence that the medial and dorsal raphe nuclei mediate serotonergically-induced increases in prolactin release from the pituitary. Brain Res 299:231-237
9. Hanley NR, Van de Kar LD 2003 Serotonin and the neuroendocrine regulation of the hypothalamic-pituitary-adrenal axis in health and disease. Vitam Horm 66:189-255
10. Murakami Y, Kato Y, Kabayama Y, Tojo K, Inoue T, Imura H 1986 Involvement of growth hormone (GH)-releasing factor in GH secretion induced by serotoninergic mechanisms in conscious rats. Endocrinology 119:1089-1092
11. Van de Kar LD, Rittenhouse PA, Li Q, Levy AD 1996 Serotonergic regulation of renin and prolactin secretion. Behav Brain Res 73:203-208
12. Vitale ML, Chiocchio SR 1993 Serotonin, a neurotransmitter involved in the regulation of luteinizing hormone release. Endocr Rev 14:480-493
13. Kondo Y, Nagatsu I, Yoshida M, Karasawa N, Nagatsu T 1983 Existence of noradrenalin cells and serotonin cells in the pituitary gland of Rana catesbeiana. Cell Tissue Res 228:405-408
14. Payette RF, Gershon MD, Nunez EA 1986 Colocalization of luteinizing hormone and serotonin in secretory granules of mammalian gonadotrophs. Anat Rec 215:51-58
15. Saavedra JM, Palkovits M, Kizer JS, Brownstein M, Zivin JA 1975 Distribution of biogenic amines and related enzymes in the rat pituitary gland. J Neurochem 25:257-260
16. Saland LC 2001 The mammalian pituitary intermediate lobe: an update on innervation and regulation. Brain Res Bull 54:587-593
17. Payette RF, Gershon MD, Nunez EA 1985 Serotonergic elements of the mammalian pituitary. Endocrinology 116:1933-1942
18. Westlund KN, Childs GV 1982 Localization of serotonin fibers in the rat adenohypophysis. Endocrinology 111:1761-1763
19. Johns MA, Azmitia EC, Krieger DT 1982 Specific in vitro uptake of serotonin by cells in the anterior pituitary of the rat. Endocrinology 110:754-760
20. Nunez EA, Gershon MD, Silverman AJ 1981 Uptake of 5-hydroxytryptamine by gonadotrophs of the bat's pituitary: a combined immunocytochemical radioautographic analysis. J Histochem Cytochem 29:1336-1346
21. Hansson SR, Mezey E, Hoffman BJ 1998 Serotonin transporter messenger RNA in the developing rat brain: early expression in serotonergic neurons and transient expression in non-serotonergic neurons. Neuroscience 83:1185-1201
22. Abou Samra AB, Fevre-Montange M, Loras B, Durand A, Tourniaire J, Bertrand J 1985 Effect of indolamines on β-endorphin release by rat anterior pituitary cells. Neuroendocrinology 41:490-493
23. Calogero AE, Bagdy G, Moncada ML, D'Agata R 1993 Effect of selective serotonin agonists on basal, corticotrophin-releasing hormone- and vasopressin-induced ACTH release in vitro from rat pituitary cells. J Endocrinol 136:381-387
24. Calogero AE, Bagdy G, Burrello N, Polosa P, D'Agata R 1995 Role for serotonin3 receptors in the control of adrenocorticotropic hormone release from rat pituitary cell cultures. Eur J Endocrinol 133:251-254
25. Fiekers JF, Konopka LM 1994 Serotonin-induced changes in membrane potential and cytosolic free calcium in a clonal pituitary cell line (AtT-20) of cultured mouse corticotropes. Life Sci 55:15-26
26. Apfelbaum ME 1987 Effect of serotonin on the basal and gonadotrophin-releasing hormone-induced release of luteinizing hormone from rat pituitary glands in vitro. Life Sci 41:2069-2076
27. Martin JE, Engel JN, Klein DC 1977 Inhibition of the in vitro pituitary response to luteinizing hormone-releasing hormone by melatonin, serotonin, and 5-methoxytryptamine. Endocrinology 100:675-680
28. Quirk PL, Siegel RE 2005 The serotonin type 3A receptor facilitates luteinizing hormone release and LHβ promoter activity in immortalized pituitary gonadotropes. Endocrine 27:37-43
29. Billiard J 1996 Functional heterogeneity of pituitary gonadotropes in response to a variety of neuromodulators. Mol Cell Endocrinol 123:163-170
30. Stobie KM, Shin SH 1983 Serotonin stimulates prolactin secretion in the hypophysectomized adenohypophyseal grafted rat. Acta Endocrinol (Copenh) 102:511-516
31. Apfelbaum ME 1987 Effect of serotonin on basal and TRH-induced release of prolactin from rat pituitary glands in vitro. Acta Endocrinol (Copenh) 114:565-571
32. Apfelbaum ME 1998 Role of vasoactive intestinal peptide and 5-HT2 receptor subtype in serotonin stimulation of basal and thyrotropin-releasing- hormone-induced prolactin release in vitro from rat pituitary cells. Neuroendocrinology 67:45-50
33. Lopez F, Gonzalez D, Aguilar E 1987 Possible direct effect of serotonin on pituitary prolactin secretion: in vivo and in vitro studies. Horm Res 25:160-170
34. Balsa JA, Sanchez-Franco F, Pazos F, Lara JI, Lorenzo MJ, Maldonado G, Cacicedo L 1998 Direct action of serotonin on prolactin, growth hormone, corticotropin and luteinizing hormone release in cocultures of anterior and posterior pituitary lobes: autocrine and/or paracrine action of vasoactive intestinal peptide. Neuroendocrinology 68:326-333
35. Johnston CA, Fagin KD, Alper RH, Negro-Vilar A 1986 Prolactin release after 5-hydroxytryptophan treatment requires an intact neurointermediate pituitary lobe. Endocrinology 118:805-810
36. Bagdy G, Calogero AE, Szemeredi K, Gomez MT, Murphy DL, Chrousos GP, Gold PW 1990 β-Endorphin responses to different serotonin agonists: involvement of corticotropin-releasing hormone, vasopressin and direct pituitary action. Brain Res 537:227-232
37. Gerard C, el Mestikawy S, Lebrand C, Adrien J, Ruat M, Traiffort E, Hamon M, Martres MP 1996 Quantitative RT-PCR distribution of serotonin 5-HT6 receptor mRNA in the central nervous system of control or 5,7- dihydroxytryptamine-treated rats. Synapse 23:164-173
38. Tecott LH, Sun LM, Akana SF, Strack AM, Lowenstein DH, Dallman MF, Julius D 1995 Eating disorder and epilepsy in mice lacking 5-HT2c serotonin receptors. Nature 374:542-546
39. Denef C, Maertens P, Allaerts W, Mignon A, Robberecht W, Swennen L, Carmeliet P 1989 Cell-to-cell communication in peptide target cells of anterior pituitary. Methods Enzymol 168:47-71
40. Denef C 2003 Paracrine control of lactotrope proliferation and differentiation. Trends Endocrinol Metab 14:188-195
41. Swinnen E, Boussemaere M, Denef C 2005 Stimulation and inhibition of prolactin release by prolactin-releasing peptide in rat anterior pituitary cell aggregates. J Neuroendocrinol 17:379-386
42. Hauspie A, Seuntjens E, Vankelecom H, Denef C 2003 Stimulation of combinatorial expression of prolactin and glycoprotein hormone α-subunit genes by gonadotropin-releasing hormone and estradiol-17β in single rat pituitary cells during aggregate cell culture. Endocrinology 144:388-399
43. Houben H, Denef C 1990 Stimulation of growth hormone and prolactin release from rat pituitary cell aggregates by bombesin- and ranatensin-like peptides is potentiated by estradiol, 5α-dihydrotestosterone, and dexamethasone. Endocrinology 126:2257-2266
44. Langouche L, Pals K, Denef C 2002 Structure-activity relationship and signal transduction of γ-MSH peptides in GH3 cells: further evidence for a new melanocortin receptor. Peptides 23:1077-1086
45. Carmeliet P, Baes M, Denef C 1989 The glucocorticoid hormone dexamethasone reverses the growth hormone-releasing properties of the cholinomimetic carbachol. Endocrinology 124:2625-2634
46. Houben H, Denef C 1992 Negative regulation by dexamethasone of the potentiation of neuromedin C-induced growth hormone and prolactin release by estradiol in anterior pituitary cell aggregates. Life Sci 50:775-780
47. Denef C, Schramme C, Baes M 1984 Influence of corticosteroids on prolactin release from anterior pituitary cell aggregates cultured in serum-free medium. Differential effects on dopamine-induced inhibition, postdopamine rebound and stimulation by TRH, vasoactive intestinal peptide (VIP), angiotensin II and isoproterenol. J Steroid Biochem 20:197-202
48. Boess FG, Martin IL 1994 Molecular biology of 5-HT receptors. Neuropharmacology 33:275-317
49. Erlander MG, Lovenberg TW, Baron BM, de Lecea L, Danielson PE, Racke M, Slone AL, Siegel BW, Foye PE, Cannon K, Burns JE, Sutcliffe JG 1993 Two members of a distinct subfamily of 5-hydroxytryptamine receptors (5HTR5) differentially expressed in rat brain. Proc Natl Acad Sci USA 90:3452-3456
50. Bonhaus DW, Bach C, DeSouza A, Salazar FH, Matsuoka BD, Zuppan P, Chan HW, Eglen RM 1995 The pharmacology and distribution of human 5-hydroxytryptamine2B (5-HT2B) receptor gene products: comparison with 5-HT2A and 5-HT2C receptors. Br J Pharmacol 115:622-628
51. Bonhaus DW, Weinhardt KK, Taylor M, DeSouza A, McNeeley PM, Szczepanski K, Fontana DJ, Trinh J, Rocha CL, Dawson MW, Flippin LA, Eglen RM 1997 RS-102221: a novel high affinity and selective, 5-HT2C receptor antagonist. Neuropharmacology 36:621-629
52. 3H]ketanserin binding sites associated with nerve terminals in rat brain and with platelets; relation with release of biogenic amine metabolites induced by ketanserin- and tetrabenazine-like drugs. J Pharmacol Exp Ther 244:310-321
53. 3H]ketanserin. Eur J Pharmacol 118:13-23
54. Hoyer D 1988 Molecular pharmacology and biology of 5-HT1C receptors. Trends Pharmacol Sci 9:89-94
55. Kennett GA, Wood MD, Bright F, Cilia J, Piper DC, Gager T, Thomas D, Baxter GS, Forbes IT, Ham P, Blackburn TP 1996 In vitro and in vivo profile of SB 206553, a potent 5-HT2C/5-HT2B receptor antagonist with anxiolytic-like properties. Br J Pharmacol 117:427-434
56. 3H]phosphatidylinositols. Naunyn-Schmiedebergs Arch Pharmacol 365:242-252
57. 2C- receptors. Naunyn-Schmiedeberg's Arch Pharmacol 370:114-123
58. Lovell PJ, Bromidge SM, Dabbs S, Duckworth DM, Forbes IT, Jennings AJ, King FD, Middlemiss DN, Rahman SK, Saunders DV, Collin LL, Hagan JJ, Riley GJ, Thomas DR 2000 A novel, potent, and selective 5-HT(7) antagonist: (R)-3-(2-(2-(4-methylpiperidin-1-yl)ethyl)pyrrolidine-1-sulfonyl) phen ol (SB-269970). J Med Chem 43:342-345
59. 3H]BIMU-1, a 5-hydroxytryptamine3 receptor ligand in NG-108 cells, selectively labels σ-2 binding sites in guinea pig hippocampus. J Pharmacol Exp Ther 267:961-970
60. Van den Wyngaert I, Gommeren W, Verhasselt P, Jurzak M, Leysen J, Luyten W, Bender E 1997 Cloning and expression of a human serotonin 5-HT4 receptor cDNA. J Neurochem 69:1810-1819
61. 3H]RS 57639, a high affinity, selective 5-HT4 receptor partial agonist, specifically labels guinea-pig striatal and rat cloned (5-HT4S and 5-HT4L) receptors. Neuropharmacology 36:671-679
62. Hirst WD, Abrahamsen B, Blaney FE, Calver AR, Aloj L, Price GW, Medhurst AD 2003 Differences in the central nervous system distribution and pharmacology of the mouse 5-hydroxytryptamine-6 receptor compared with rat and human receptors investigated by radioligand binding, site-directed mutagenesis, and molecular modeling. Mol Pharmacol 64:1295-1308
63. Johansson L, Sohn D, Thorberg SO, Jackson DM, Kelder D, Larsson LG, Renyi L, Ross SB, Wallsten C, Eriksson H, Hu PS, Jerning E, Mohell N, Westlind-Danielsson A 1997 The pharmacological characterization of a novel selective 5-hydroxytryptamine1A receptor antagonist, NAD-299. J Pharmacol Exp Ther 283:216-225
64. Millan MJ, Maiofiss L, Cussac D, Audinot V, Boutin JA, Newman-Tancredi A 2002 Differential actions of antiparkinson agents at multiple classes of monoaminergic receptor. I. A multivariate analysis of the binding profiles of 14 drugs at 21 native and cloned human receptor subtypes. J Pharmacol Exp Ther 303:791-804
65. Price GW, Burton MJ, Collin LJ, Duckworth M, Gaster L, Gothert M, Jones BJ, Roberts C, Watson JM, Middlemiss DN 1997 SB-216641 and BRL-15572-compounds to pharmacologically discriminate h5-HT1B and h5-HT1D receptors. Naunyn-Schmiedebergs Arch Pharmacol 356:312-320
66. 3H]5-hydroxytryptamine labels the agonist high affinity state of the cloned rat 5-HT4 receptor. Eur J Pharmacol 304:231-235
67. Wainscott DB, Lucaites VL, Kursar JD, Baez M, Nelson DL 1996 Pharmacologic characterization of the human 5-hydroxytryptamine2B receptor: evidence for species differences. J Pharmacol Exp Ther 276:720-727
68. Herrick-Davis K, Grinde E, Gauthier C, Teitler M 1998 Pharmacological characterization of the constitutively activated state of the serotonin 5-HT2C receptor. Ann NY Acad Sci 861:140-145
69. Egan C, Grinde E, Dupre A, Roth BL, Hake M, Teitler M, Herrick-Davis K 2000 Agonist high and low affinity state ratios predict drug intrinsic activity and a revised ternary complex mechanism at serotonin 5-HT(2A) and 5-HT(2C) receptors. Synapse 35:144-150
70. Lovenberg TW, Erlander MG, Baron BM, Racke M, Slone AL, Siegel BW, Craft CM, Burns JE, Danielson PE, Sutcliffe JG 1993 Molecular cloning and functional expression of 5-HT1E-like rat and human 5-hydroxytryptamine receptor genes. Proc Natl Acad Sci USA 90:2184-2188
71. Wisden W, Parker EM, Mahle CD, Grisel DA, Nowak HP, Yocca FD, Felder CC, Seeburg PH, Voigt MM 1993 Cloning and characterization of the rat 5-HT5B receptor: evidence that the 5-HT5B receptor couples to a G protein in mammalian cell membranes. FEBS Lett 333:25-31
72. Ruat M, Traiffort E, Leurs R, Tardivel-Lacombe J, Diaz J, Arrang JM, Schwartz JC 1993 Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation. Proc Natl Acad Sci USA 90:8547-8551
73. Shen Y, Monsma Jr FJ, Metcalf MA, Jose PA, Hamblin MW, Sibley DR 1993 Molecular cloning and expression of a 5-hydroxytryptamine7 serotonin receptor subtype. J Biol Chem 268:18200-18204
74. i family. Br J Pharmacol 136:1072-1078
75. o proteins and inhibits adenylate cyclase in HEK 293 cells. Eur J Pharmacol 361:299-309
76. Fuller RW, Wong DT, Robertson DW 1991 Fluoxetine, a selective inhibitor of serotonin uptake. Med Res Rev 11:17-34
77. Hyttel J 1982 Citalopram - pharmacological profile of a specific serotonin uptake inhibitor with antidepressant activity. Prog Neuropsychopharmacol Biol Psychiat 6:277-295
78. Darchen F, Scherman D, Laduron PM, Henry JP 1988 Ketanserin binds to the monoamine transporter of chromaffin granules and of synaptic vesicles. Mol Pharmacol 33:672-677
79. Zolkowska D, Rothman RB, Baumann MH 2006 Amphetamine analogs increase plasma serotonin: implications for cardiac and pulmonary disease. J Pharmacol Exp Ther 318:604-610
80. Herve P, Launay JM, Scrobohaci ML, Brenot F, Simonneau G, Petitpretz P, Poubeau P, Cerrina J, Duroux P, Drouet L 1995 Increased plasma serotonin in primary pulmonary hypertension. Am J Med 99:249-254
81. Callebert J, Esteve JM, Herve P, Peoc'h K, Tournois C, Drouet L, Launay JM, Maroteaux L 2006 Evidence for a control of plasma serotonin levels by 5-hydroxytryptamine2b receptors in mice. J Pharmacol Exp Ther 317:724-731
82. Denef C, Schramme C 1985 Regulation of prolactin release by angiotensin. Horm Res 22:135-141
83. Robberecht W, Denef C 1988 Enhanced ANG II activity in anterior pituitary cell aggregates from hypertensive rats. Am J Physiol 255:R407-R411
84. Grossman CJ, Kilpatrick GJ, Bunce KT 1993 Development of a radioligand binding assay for 5-HT4 receptors in guinea-pig and rat brain. Br J Pharmacol 109:618-624
85. Hoyer D, Clarke DE, Fozard JR, Hartig PR, Martin GR, Mylecharane EJ, Saxena PR, Humphrey PP 1994 International Union of Pharmacology classification of receptors for 5-hydroxytryptamine (serotonin). Pharmacol Rev 46:157-203
86. Bockaert J, Sebben M, Dumuis A 1990 Pharmacological characterization of 5-hydroxytryptamine4(5-HT4) receptors positively coupled to adenylate cyclase in adult guinea pig hippocampal membranes: effect of substituted benzamide derivatives. Mol Pharmacol 37:408-411
87. Uchiyama-Tsuyuki Y, Saitoh M, Muramatsu M 1996 Identification and characterization of the 5-HT4 receptor in the intestinal tract and striatum of the guinea pig. Life Sci 59:2129-2137
88. Wardle KA, Sanger GJ 1993 The guinea-pig distal colon - a sensitive preparation for the investigation of 5-HT4 receptor-mediated contractions. Br J Pharmacol 110:1593-1599
89. Kurose H, Katada T, Amano T, Ui M 1983 Specific uncoupling by islet-activating protein, pertussis toxin, of negative signal transduction via α-adrenergic, cholinergic, and opiate receptors in neuroblastoma x glioma hybrid cells. J Biol Chem 258:4870-4875
90. Bluet Pajot MT, Mounier F, Di Sciullo A, Schmidt B, Kordon C 1995 Differential sites of action of 8OHDPAT, a 5HT1A agonist, on ACTH and PRL secretion in the rat. Neuroendocrinology 61:159-166
91. Masand PS, Gupta S 1999 Selective serotonin-reuptake inhibitors: an update. Harv Rev Psychiatry 7:69-84
92. Broer S 2006 The SLC6 orphans are forming a family of amino acid transporters. Neurochem Int 48:559-567
93. Medhurst AD, Lezoualc&rsquo, h F, Fischmeister R, Middlemiss DN, Gareth J 2001 Quantitative mRNA analysis of five C-terminal splice variants of the human 5-HT4 receptor in the central nervous system by TaqMan real time RT-PCR. Brain Res Mol Brain Res 90:125-134
94. Berglund CM, Aarum J, Haeberlein SL, Nyengaard JR, Hokfelt T, Sandberg K, Naslund J, Persson MA 2004 Characterization of long-term mouse brain aggregating cultures: evidence for maintenance of neural precursor cells. J Comp Neurol 474:246-260
95. Ferrera D, Poggi S, Biassoni C, Dickson GR, Astigiano S, Barbieri O, Favre A, Franzi AT, Strangio A, Federici A, Manduca P 2002 Three-dimensional cultures of normal human osteoblasts: proliferation and differentiation potential in vitro and upon ectopic implantation in nude mice. Bone 30:718-725
96. Flouriot G, Vaillant C, Salbert G, Pelissero C, Guiraud JM, Valotaire Y 1993 Monolayer and aggregate cultures of rainbow trout hepatocytes: long-term and stable liver-specific expression in aggregates. J Cell Sci 105:407-416
97. Goossens K, Deboel L, Swinnen JV, Roskams T, Manin M, Rombauts W, Verhoeven G 2002 Both retinoids and androgens are required to maintain or promote functional differentiation in reaggregation cultures of human prostate epithelial cells. Prostate 53:34-49
98. Miao D, Murant S, Scutt N, Genever P, Scutt A 2004 Megakaryocyte-bone marrow stromal cell aggregates demonstrate increased colony formation and alkaline phosphatase expression in vitro. Tissue Eng 10:807-817
99. Vollmer G, Layer PG 1986 An in vitro model of proliferation and differentiation of the chick retina: coaggregates of retinal and pigment epithelial cells. J Neurosci 6:1885-1896
100. Watzka SB, Steiner M, Samorapoompichit P, Gross K, Coles JG, Wolner E, Weigel G 2004 Establishment of vessel-like structures in long-term three-dimensional tissue culture of myocardium: an electron microscopy study. Tissue Eng 10:1684-1694
101. Bethea CL, Mirkes SJ, Shively CA, Adams MR 2000 Steroid regulation of tryptophan hydroxylase protein in the dorsal raphe of macaques. Biol Psychiat 47:562-576
102. Koldzic-Zivanovic N, Seitz PK, Watson CS, Cunningham KA, Thomas ML 2004 Intracellular signaling involved in estrogen regulation of serotonin reuptake. Mol Cell Endocrinol 226:33-42
103. McQueen JK, Wilson H, Fink G 1997 Estradiol-17β increases serotonin transporter (SERT) mRNA levels and the density of SERT-binding sites in female rat brain. Brain Res Mol Brain Res 45:13-23
104. Sumner BE, Grant KE, Rosie R, Hegele-Hartung C, Fritzemeier KH, Fink G 1999 Effects of tamoxifen on serotonin transporter and 5-hydroxytryptamine(2A) receptor binding sites and mRNA levels in the brain of ovariectomized rats with or without acute estradiol replacement. Brain Res Mol Brain Res 73:119-128
105. Lieberman ME, Maurer RA, Claude P, Wiklund J, Wertz N, Gorski J 1981 Regulation of pituitary growth and prolactin gene expression by estrogen. Adv Exp Med Biol 138:151-163
106. Bennett JR 1989 How safe and acceptable is cisapride? Scand J Gastroenterol Suppl 165:59-61