Green autofluorescence of dinoflagellate cysts can be used instead of primuline staining for cyst visualization and enumeration in sediments

Journal of Phycology

Volumn 43, Issue 1, 2007, Pages 65-68

  Tang, Ying Zhong ^a   Dobbs, Fred C ^a  

^a Old Dominion University   (United States)

Author keywords

Dinoflagellate cyst; Green autofluorescence; Gymnodinium catenatum; Primuline staining; Scrippsiella trochoidea

Indexed keywords

DINOPHYCEAE; GYMNODINIUM CATENATUM; SCRIPPSIELLA TROCHOIDEA;

EID: 33846934008     PISSN: 00223646     EISSN: 15298817     Source Type: Journal    
DOI: 10.1111/j.1529-8817.2006.00306.x     Document Type: Article

References (15)

1. Anderson, D. M., Fukuyo, Y. & Matsuoka, K. 2003. Cyst methodologies. In Hallegraeff, G. M., Anderson, D. M. & Cembella, A. D. [Eds.] Manual on Harmful Marine Microalgae. Monographs on Oceanographic Methodology. 11th ed. UNESCO, Paris, pp. 165-90.
2. Anderson, D. M., Keafer, B. A., McGillicuddy, D. J., Mickelson, M. J., Keay, K. E., Scott Libby, P., Manning, J. P., Mayo, C. A., Whittaker, D. K., Michael Hickey, J., He, R. Y., Lynch, D. R. & Smith, K. W. 2005a. Initial observations of the 2005 Alexandrium fundyense bloom in southern New England: general patterns and mechanisms. Deep-Sea Res. Part II Top. Stud. Oceanogr. 52(Special issue):2856-76.
3. Anderson, D. M., Stock, C. A., Keafer, B. A., Bronzino Nelson, A., McGillicuddy, D. J., Keller, M., Thompson, B., Matrai, P. A. & Martin, J. 2005b. Alexandrium fundyense cyst dynamics in the Gulf of Maine. Deep-Sea Res. Part II Top. Stud. Oceanogr. 52 (Special issue):2522-42.
4. Bolch, C. J. S. 1997. The use of sodium polytungstate for the separation and concentration of living dinoflagellate cysts from marine sediments. Phycologia 36:472-8.
5. Cho, H. J. & Matsuoka, K. 2001. Distribution of dinoflagellate cysts in surface sediments from the Yellow Sea and East China Sea. Mar. Micropaleontol. 42:101-23.
6. Doblin, M. A., Blackburn, S. I. & Hallegraeff, G. M. 1999. Growth and biomass stimulation of the toxic dinoflagellate Gymnodinium catenatum (Graham) by dissolved organic substances. J. Exp. Mar. Biol. Ecol. 236:33-47.
7. Elbrächter, M. 1994. Green autofluorescence-a new taxonomic feature for living dinoflagellate cysts and vegetative cells. Rev. Palaeobot. Palynol. 84:101-5.
8. Kirn, S. L., Townsend, D. W. & Pettigrew, N. R. 2005. Suspended Alexandrium spp. hypnozygote cysts in the Gulf of Maine. Deep-Sea Res. Part II Top. Stud. Oceanogr. 52(Special issue): 2543-59.
9. Matsuoka, K. & Fukuyo, Y. 2000. Technical Guide for Modern Dino-flagellate Cyst Study. WESTPAC-HAB/WESTPAC/IOC, Tokyo, Japan, pp. 5-8.
10. McGillicuddy, D. J., Signell, R. P., Stock, C. A., Keafer, B. A., Keller, M. D., Hetland, R. D. & Anderson, D. M. 2003. A mechanism for offshore initiation of harmful algal blooms in the coastal Gulf of Maine. J. Plankton Res. 25:1131-8.
11. Rengefors, K. & Anderson, D. M. 1998. Environmental and endogenous regulation of cyst germination in two freshwater dinoflagellates. J. Phycol. 34:568-77.
12. Tsujino, M., Kamiyama, T., Uchida, T., Yamaguchi, M. & Itakura, S. 2002. Abundance and germination capability of resting cysts of Alexandrium spp. (Dinophyceae) from faecal pellets of macrobenthic organisms. J. Exp. Mar. Biol. Ecol. 271:1-7.
13. Yamaguchi, M., Itakura, S. & Imai, I. 1995a. Vertical and horizontal distribution and abundance of resting cysts of the toxic dinoflagellate Alexandrium tamarense and Alexandrium catenella in sediments of Hiroshima Bay, the Seto Inland Sea, Japan. Nippon Suisan Gakkaishi 61:700-6.
14. Yamaguchi, M., Itakura, S., Imai, I. & Ishida, Y. 1995b. A rapid and precise technique for enumeration of resting cysts of Alexandrium spp. (Dinophyceae) in natural sediments. Phycologia 34:207-14.
15. Yih, W. & Coats, D. W. 2000. Infection of Gymnodinium sanguineum by the dinoflagellate Amoebophrya sp.: effect of nutrient environment on parasite generation time, reproduction, and infectivity. J. Eukaryot. Microbiol. 47:504-10.