Detection of UV-induced thymine dimers in individual Cryptosporidium parvum and Cryptosporidium hominis oocysts by immunofluorescence microscopy

Applied and Environmental Microbiology

Volumn 73, Issue 3, 2007, Pages 947-955

  Al Adhami, B H ^a   Nichols, R A B ^a   Kusel, J R ^b   O'Grady, J ^c   Smith, H V ^a  

^a STOBHILL HOSPITAL   (United Kingdom)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

^c UNIVERSITY OF STRATHCLYDE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM PARVUM; IMMUNOFLUORESCENCE MICROSCOPY; OOCYSTS;

DIMERS; DNA; FLUORESCENCE; MICROSCOPIC EXAMINATION; MONOCLONAL ANTIBODIES; NITROGEN COMPOUNDS; RADIATION EFFECTS; ULTRAVIOLET RADIATION;

PROTOZOA;

4',6' DIAMIDINO 2 PHENYLINDOLE; CYCLOBUTANE DERIVATIVE; DNA; INDOLE; MONOCLONAL ANTIBODY; THYMINE DERIVATIVE; UNCLASSIFIED DRUG;

ANTIBODY; CYST; DETECTION METHOD; LIGHT EFFECT; ORGANIC COMPOUND; PROTOZOAN; ULTRAVIOLET RADIATION;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM PARVUM; DIMERIZATION; IMMUNOFLUORESCENCE MICROSCOPY; IN VITRO STUDY; MOUSE; NEWBORN; NONHUMAN; OOCYST; SPOROZOITE; ULTRAVIOLET RADIATION;

ANIMALS; ANIMALS, NEWBORN; ANTIBODIES; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; DNA DAMAGE; MICE; MICROSCOPY, FLUORESCENCE; OOCYSTS; PYRIMIDINE DIMERS; ULTRAVIOLET RAYS;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM PARVUM; MUS;

EID: 33846933028     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01251-06     Document Type: Article

References (28)

1. Anonymous. 1999. Isolation and identification of Cryptosporidium oocysts and Giardia cysts in waters, 1999. Methods for the examination of waters and associated materials. HMSO, London, United Kingdom.
2. Anonymous. 1999. The water supply (water quality) (amendment). Regulations 1999, SI no. 1524. Drinking Water Inspectorate, London, United Kingdom.
3. Anonymous. 2005. Standard operating protocol for the monitoring of Cryptosporidium oocysts in treated water supplies to satisfy The Water Supply (Water Quality) Regulations 200, SI no. 3184 England/The Water Supply (Water Quality) Regulations 2001, SI no. 3911 (W.323) Wales. Part 2. Laboratory and analytical procedures. Drinking Water Inspectorate, London, United Kingdom. http://www.dwi.gov.uk/regs/crypto/pdf/sop%20part%202.pdf.
4. Beloseric, M., S. A. Craik, J. L. Stafford, N. F. Neumann, J. Kruithof, and D. W. Smith. 2001. Studies on the resistance/reactivation of Giardia muris cysts and Cryptosporidium parvum oocysts exposed to medium-pressure ultraviolet irradiation. FEMS Microbiol. Lett. 204:197-204.
5. Bukhari, Z., and H. V. Smith. 1995. Effect of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine feces. J. Clin. Microbiol. 33:2592-2595.
6. Campbell, A. T., L. J. Robertson, and H. V. Smith. 1992. Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes. Appl. Environ. Microbiol. 58:3488-3493.
7. Craik, S. A., D. Weldon, G. R. Finch, J. R. Bolton, and M. Beloseric. 2001. Inactivation of Cryptosporidium parvum oocysts using medium- and low-pressure ultraviolet radiation. Water Res. 35:1387-1398.
8. Hijnen, W. A. M., E. F. Beerendonk, and G. J. Medema. 2006. Inactivation credit of UV irradiation for viruses, bacteria and protozoan (oo)cysts in water: a review. Water Res. 40:3-22.
9. Homan, W., T. Van Gorkom, Y. Y. Kan, and J. Hepener. 1999. Characterization of Cryptosporidium parvum in human and animal feces by single-tube nested polymerase chain reaction and restriction analysis. Parasitol. Res. 85:707-712.
10. Johnson, A. M., K. Linden, K. M. Ciociola, R. De Leon, G. Widmer, and P. A. Rochelle. 2005. UV inactivation of Cryptosporidium hominis as measured in cell culture. Appl. Environ. Microbiol. 71:2800-2802.
11. Korich, D. G., M. M. Marshall, H. V. Smith, J. E. O'Grady, Z. Bukhari, C. R. Flicker, J. P. Rosen, and J. L. Clancy. 2000. Inter-laboratory comparison of the CD-1 neonatal mouse logistic dose-response model for Cryptosporidium parvum oocysts. J. Eukaryot. Microbiol. 47:294-298.
12. Mitchell, D. L. 1988. The relative cytotoxicity of (6-4) photoproducts and cyclobutane dimers in mammalian cells. Photochem. Photobiol. 48:51-57.
13. Morita, S., A. Namikoshi, T. Hirata, K. Oguma, H. Katayama, S. Ohgaki, N. Motoyama, and M. Fujiwara. 2002. Efficacy of UV irradiation in inactivating Cryptosporidium parvum oocysts. Appl. Environ Microbiol. 68:5387-5393.
14. Nichols, R. A. B., and H. V. Smith. 2004. Optimization of DNA extraction and molecular detection of Cryptosporidium oocysts in natural mineral water sources. J. Food Prot. 67:524-532.
15. Nichols, R. A. B., B. M. Campbell, and H. V. Smith. 2003. Identification of Cryptosporidium spp. oocysts in United Kingdom noncarbonated natural mineral waters and drinking waters by using a modified nested PCR-restriction fragment length polymorphism assay. Appl. Environ. Microbiol. 69:4183-4189.
16. Oguma, K., H. katayama, H. Mitani, S. Morita, T. Hirata, and S. Ohgaki. 2001. Determination of pyrimidine dimers in Escherichia coli and Cryptosporidium parvum during UV light inactivation, photoreactivation, and dark repair. Appl. Environ. Microbiol. 67:4630-4637.
17. Robertson, L. J., A. T. Campbell, and H. V. Smith. 1993. In vitro excystation of Cryptosporidium parvum. Parasitology 106:13-19.
18. Rochelle, P. A., D. Fallar, M. M. Marshall, B. A. Montelone, S. J. Upton, and K. Woods. 2004. Irreversible UV inactivation of Cryptosporidium spp. despite the presence of repair genes. J. Eukaryot. Microbiol. 51:553-562.
19. Rochelle, P. A., S. J. Upton, B. A. Montelon, and K. Woods. 2005. The response of Cryptosporidium to UV light. Trends Parasitol. 21:81-87.
20. Rothschild, L. J. 1999. The influence of UV radiation on protistan evolution. J. Eukaryot. Microbiol. 46:548-555.
21. Roza, L., F. R. De Gruijl, J. B. A. Bergen Henegouwen, K. Guikers, H. Van Weelden, G. P. Van Der Schans, and R. A. Baan. 1991. Detection of photorepair of UV-induced thymine dimers in human epidermis by immunofluorescence microscopy. J. Investig. Dermatol. 96:903-907.
22. Shin, G. A., K. G. Linden, M. J. Arrowood, and M. D. Sobsey. 2001. Low-pressure UV inactivation and DNA repair potential of Cryptosporidium parvum oocysts. Appl. Environ. Microbiol. 67:3029-3032.
23. Smith, H. V., B. M. Campbell, C. A. Paton, and R. A. B. Nichols. 2002. Significance of enhanced morphological detection of Cryptosporidium sp. oocysts in environmental water concentrates using DAPI and immunofluorescence microscopy. Appl. Environ. Microbiol. 68:5198-5201.
24. U.S. Environmental Protection Agency. 2001. Method 1622: Cryptosporidium in water by filtration/IMS/FA, EPA 821-R01-026. Office of Water, U.S. Environmental Protection Agency, Washington, DC.
25. U.S. Environmental Protection Agency. 2001. Method 1623: Giardia and Cryptosporidium in water by filtration/IMS/FA, EPA 821-R01-025. Office of Water, U.S. Environmental Protection Agency, Washington, DC.
26. Widmer, G., D. Akiyoshi, M. A. Buckholt, X. Feng, S. M. Rich, K. M. Deary, C. A. Bowman, P. Xu, Y. Wang, G. A. Buck, and S. Tzipori. 2000. Animal propagation and genomic survey of a genotype 1 isolate of Cryptosporidium parvum. Mol. Biochem. Parasitol. 108:187-197.
27. Xiao, L., L. Escalante, C. Young, I. Sulaiman, A. A. Escalante, J. R. Montali, R. Fayer, and A. A. Lal. 1999. Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl. Environ. Microbiol. 65:1578-1583.
28. Xiao, L., K. Alderisio, J. Limor, M. Royer, and A. A. Lal. 2000. Identification of species and sources of Cryptosporidium oocysts in storm waters with a small-subunit rRNA-based diagnostic and genotyping tool. Appl. Environ. Microbiol. 66:5492-5498.