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Volumn 7, Issue 1, 2007, Pages 155-160

Effects of mechanical flexion on the penetration of fullerene amino acid-derivatized peptide nanoparticles through skin

Author keywords

[No Author keywords available]

Indexed keywords

DERMAL PENETRATION; FULLERENE-SUBSTITUTED PHENYLALANINE; NUCLEAR LOCALIZATION; PEPTIDE NANOPARTICLES;

EID: 33846883504     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl062464m     Document Type: Article
Times cited : (285)

References (28)
  • 10
    • 33846873919 scopus 로고    scopus 로고
    • Using preloaded Fmoc-Val-Wang resin (491 mg, 0.30 mmol) as solid phase, each coupling used 4 fold amino acid excess, and HBTU, HOBT as activators and DIEA as base in a 1:1:1:3 ratio. Fmoc deprotection was performed using 25% perperidine in DMF solution. After the NILS sequence (Pro-Lys-Lys-Lys-Arg-Lys- Val) was completed, a Lys(Mtt) residue was coupled to the end. One sixth of the resin was placed in a 25 mL fritted glass tube, swollen with DMF. A 3-fold excess of N-Boc-Baa (157 mg, 0.15 mM) dissolved in DMF/ DCM (9 mL, 2:1) was activated with PyBOP/HOBt/DIEA (1:1:1:3) for 2 minutes, then mixed with the resin in the fritted glass tube, and shaken on an automated shaker for 1 day at room temperature. The resin was washed thoroughly with DMF and DCM to remove any unreacted N-Boc-Baa. The resin was washed with DCM for complete removal of DMF. To achieve the maximum cleavage of Mtt protecting group, the resin was shrunk with MeOH twice. When the resin was treated with 1% TFA and 5% TIPS in
    • -1 flow rate. The elution time was 37 min. After purification 59.1 mg (50.6%) was recovered.
  • 12
    • 33846892278 scopus 로고    scopus 로고
    • + + H-FTTC]; found 2337, 1948.
    • + + H-FTTC]; found 2337, 1948.
  • 14
    • 0002997210 scopus 로고    scopus 로고
    • Pond, W. G, Mersmann, H. G, Eds, Cornell University Press: Ithaca, NY
    • Monteiro-Riviere, N. A. In The Biology of the Domestic Pig; Pond, W. G., Mersmann, H. G., Eds.; Cornell University Press: Ithaca, NY, 2001; pp 585-624.
    • (2001) The Biology of the Domestic Pig , pp. 585-624
    • Monteiro-Riviere, N.A.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.