High inoculation cell density could accelerate the differentiation of human bone marrow mesenchymal stem cells to chondrocyte cells

Journal of Bioscience and Bioengineering

Volumn 103, Issue 1, 2007, Pages 98-100

  Takagi, Mutsumi ^a   Umetsu, Yousuke ^a   Fujiwara, Masashi ^a   Wakitani, Shigeyuki ^b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b OSAKA CITY UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

cell density; chondrocyte; differentiation; mesenchymal stem cell

Indexed keywords

BIOMEDICAL ENGINEERING; CELL CULTURE; KETONES; RNA; TISSUE;

CELL DENSITY; CHONDROCYTES; DIFFERENTIATION; MESENCHYMAL STEM CELLS (MSC);

CELLS;

AGGRECAN; DEXAMETHASONE; MESSENGER RNA; SOMATOMEDIN C; TRANSFORMING GROWTH FACTOR BETA3;

ARTICLE; CARTILAGE; CARTILAGE CELL; CELL CULTURE; CELL DENSITY; CELL DIFFERENTIATION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; INOCULATION; MESENCHYMAL STEM CELL;

BONE MARROW CELLS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; CHONDROCYTES; CHONDROGENESIS; HUMANS; MESENCHYMAL STEM CELLS; TISSUE ENGINEERING;

EID: 33846840874     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.103.98     Document Type: Article

References (4)

1. Matsuda C., Takagi M., Hattori T., Wakitani S., and Yoshida T. Differentiation of human bone marrow mesenchymal stem cells to chondrocytes for construction of three-dimensional cartilage tissue. Cytotechnology 47 (2005) 11-17
2. Takagi M., Iemoto N., and Yoshida T. Effect of concentrations of murine stromal and hematopoietic cells on their three-dimensional coculture in nonwoven fabrics. J. Biosci. Bioeng. 94 (2002) 365-367
3. Takagi M., Nakamura T., Matsuda C., Hattori T., Wakitani S., and Yoshida T. In vitro proliferation of human bone marrow mesenchymal stem cells employing donor serum and basic fibroblast growth factor. Cytotechnology 43 (2003) 89-96
4. Takagi M., Horii K., and Yoshida T. Effect of pore diameter of porous membrane on progenitor content during a membrane-separated coculture of hematopoietic cells and stromal cell line. J. Artif. Organs 6 (2003) 130-137