Amino acid substitutions at multiple sites within the vaccinia virus D13 scaffold protein confer resistance to rifampicin

Virology

Volumn 359, Issue 1, 2007, Pages 227-232

  Charity, James C ^a   Katz, Ehud ^a   Moss, Bernard ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

Drug resistant mutants; Poxvirus; Rifampicin

Indexed keywords

D13 PROTEIN; RIFAMPICIN; SCAFFOLD PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; GENE MUTATION; GENE SEQUENCE; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VACCINIA VIRUS; VIRUS REPLICATION; WILD TYPE;

AMINO ACID SUBSTITUTION; ANTIVIRAL AGENTS; DNA MUTATIONAL ANALYSIS; DNA, VIRAL; DRUG RESISTANCE, VIRAL; MUTAGENESIS; MUTATION, MISSENSE; PLAQUE ASSAY; POLYMERASE CHAIN REACTION; RIFAMPIN; SEQUENCE ANALYSIS, DNA; VACCINIA VIRUS; VIRAL PROTEINS;

POXVIRIDAE; VACCINIA VIRUS;

EID: 33846837294     PISSN: 00426822     EISSN: 10960341     Source Type: Journal    
DOI: 10.1016/j.virol.2006.09.031     Document Type: Article

References (17)

1. r 62,000 polypeptide. Virology 156 (1987) 138-145
2. Dales S., and Siminovitch L. The development of vaccinia virus in Earle's L strain cells as examined by electron microscopy. J. Biophys. Biochem. Cytol. 10 (1961) 475-503
3. de Harven E., and Yohn D.S. The fine structure of monkey tumor poxvirus. Cancer Res. 26 (1966) 995-1008
4. Grimley P.M., Rosenblum E.N., Mims S.J., and Moss B. Interruption by rifampin of an early stage in vaccinia virus morphogenesis: accumulation of membranes which are precursors of virus envelopes. J. Virol. 6 (1970) 519-533
5. Heuser J. Deep-etch EM reveals that the early poxvirus envelope is a single membrane bilayer stabilized by a geodetic "honeycomb" surface coat. J. Cell Biol. 169 (2005) 269-283
6. Iyer L.M., Aravind L., and Koonin E.V. Common origin of four diverse families of large eukaryotic DNA viruses. J. Virol. 75 (2001) 11720-11734
7. Jin D.J., and Gross C.A. Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. J. Mol. Biol. 202 (1988) 45-58
8. McNulty-Kowalczyk A., and Paoletti E. Mutations in ORF D13L and other genetic loci alter the rifampicin phenotype of vaccinia virus. Virology 194 (1993) 638-646
9. Miner J.N., and Hruby D.E. Rifampicin prevents virosome localization of L65, an essential vaccinia virus polypeptide. Virology 170 (1989) 227-237
10. Mohandas A.R., and Dales S. Involvement of spicules in the formation of vaccinia virus envelopes elucidated by a conditional lethal mutant. Virology 214 (1995) 494-502
11. Moss B., Katz E., and Rosenblum E.N. Vaccinia virus directed RNA and protein synthesis in the presence of rifampicin. Biochem. Biophys. Res. Commun. 36 (1969) 858-865
12. Moss B., Rosenblum E.N., Katz E., and Grimley P.M. Rifampicin: a specific inhibitor of vaccinia virus assembly. Nature 224 (1969) 1280-1284
13. Nagayama A., Pogo B.G.T., and Dales S. Biogenesis of vaccinia: separation of early stages from maturation by means of rifampicin. Virology 40 (1970) 1039-1051
14. Sodeik B., Griffiths G., Ericsson M., Moss B., and Doms R.W. Assembly of vaccinia virus: effects of rifampin on the intracellular distribution of viral protein p65. J. Virol. 68 (1994) 1103-1114
15. Szajner P., Weisberg A.S., Lebowitz J., Heuser J., and Moss B. External scaffold of spherical immature poxvirus particles is made of protein trimers, forming a honeycomb lattice. J. Cell Biol. 170 (2005) 971-981
16. Tartaglia J., Piccini A., and Paoletti E. Vaccinia virus rifampicin-resistance locus specifies a late 63,000 Da gene product. Virology 150 (1986) 45-54
17. Zhang Y., and Moss B. Immature viral envelope formation is interrupted at the same stage by lac operator-mediated repression of the vaccinia virus D13L gene and by the drug rifampicin. Virology 187 (1992) 643-653