Development and assessment of a rapid method to detect Escherichia coli O26, O111 and O157 in retail minced beef

International Journal of Hygiene and Environmental Health

Volumn 210, Issue 2, 2007, Pages 155-161

  Murphy, Mary ^a,b   Carroll, Anne ^a   Walsh, Ciara ^b   Whyte, Paul ^b   O'Mahony, Micheál ^b,c   Anderson, Wayne ^c   McNamara, Eleanor ^a   Fanning, Séamus ^b  

^a Cherry Orchard Hospital Dublin   (Ireland)

^b UNIVERSITY COLLEGE DUBLIN   (Ireland)

^c FOOD SAFETY AUTHORITY OF IRELAND   (Ireland)

Author keywords

E. coli O157; Non O157 E. coli; O26 and O111; Rapid detection in food; Serogroup specific PCR

Indexed keywords

ESCHERICHIA COLI; GLYCINE MAX;

EID: 33846805641     PISSN: 14384639     EISSN: 1618131X     Source Type: Journal    
DOI: 10.1016/j.ijheh.2006.09.004     Document Type: Article

References (18)

1. Batson, S.D., Murinda, S.E., Nguyen, L.T., Oliver, S.P., 2002. Phenotypic and genetic characterization of E. coli O26 isolates from North America using carbohydrate fermentation and genes encoding virulence factors. 〈http://animalscience.ag.utk.edu/annual_reports_all.htm〉 (last accessed on the 15th of August, 2006).
2. Beebakhee G., Louie M., De Azavedo J., and Brunton J. Cloning and nucleotide sequence of the eae gene homologue from enterohemorrhagic E. coli serotype O157:H7. FEMS Microbiol. Lett. 70 (1992) 63-68
3. Bettleheim K.A., Whipp M., Djordjevic S.P., and Ramachandran V. First isolation outside Europe of sorbitol-fermenting verocytotoxigenic E. coli (VTEC) belonging to O group O157. J. Med. Microbiol. 51 (2002) 713-714
4. Debroy C., Roberts E., Kundrat J., Davis M.A., Briggs C.E., and Fratamico P.M. Detection of E. coli serogroups O26 and O113 by PCR amplification of the wzx and wzy genes. Appl. Environ. Microbiol. 70 (2004) 1830-1832
5. Gannon V.P., King R.K., Kim J.Y., and Thomas E.J. Rapid and sensitive method for detection of Shiga-like toxin-producing E. coli in ground beef using the polymerase chain reaction. Appl. Environ. Microbiol. 58 (1992) 3809-3815
6. Hiramatsu R., Matsumoto M., Miwa Y., Suzuki Y., Saito M., and Miyazaki Y. Characterization of shiga toxin-producing E. coli O26 strains and establishment of selective isolation media for these strains. J. Clin. Microbiol. 40 (2002) 922-925
7. Karch H., Bohm H., Schmidt H., Gunzer F., Aleksic S., and Heesemann J. Clonal structure and pathogenicity of shiga-like toxin-producing, sorbitol-fermenting E. coli O157:H. J. Clin. Microbiol. 31 (1993) 1200-1205
8. Karch H., and Bielaszewska M. Sorbitol-fermenting shiga-toxin-producing E. coli O157:H- strains: epidemiology, phenotypic and molecular characteristics and microbiological diagnosis. J. Clin. Microbiol. 39 (2001) 2043-2049
9. Louie M., Read S., Simor A.E., Holland J., Louie L., Ziebell K., Brunton J., and Hii J. Application of multiplex PCR for detection of non-O157 verocytotoxin-producing E. coli in bloody stools: identification of serogroups O26 and O111. J. Clin. Microbiol. 36 (1998) 3375-3377
10. McMaster C., Roch E.A., Willshaw G.A., Doherty A., Kinnear W., and Cheasty T. Verocytotoxin-producing E. coli serotype O26:H11 outbreak in an Irish crèche. Eur. J. Clin. Microbiol. Infect. Dis. 20 (2001) 430-432
11. Murphy M., Carroll A., Whyte P., Mahony M., Anderson W., McNamara E., and Fanning S. Prevalence and characterization of E. coli O26 and O111 in retail minced beef in Ireland. Foodborne Pathogens Disease 2 (2005) 357-360
12. O'Hanlon K.A., Catarame T.M.G., Duffy G., Blair I.S., and McDowell D.A. Rapid detection and quantification of E. coli O157/O26/O111 in minced beef by real-time PCR. J. Appl. Microbiol. 96 (2004) 1013-1023
13. Paton A.W., and Paton J.C. Detection and characterization of shiga toxigenic E. coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. J. Clin. Microbiol. 36 (1998) 598-602
14. Riley L.W., Remis R.S., Helgerson S.D., McGee H.B., Wells J.G., Davis B.R., Hebert R.J., Olcott E.S., Johnson L.M., Hargrett N.T., Blake P.A., and Cohen M.L. Hemorrhagic colitis associated with a rare E. coli serotype. N. Engl. J. Med. 308 (1983) 681-685
15. Safarikova M., and Safarik I. Immunomagnetic separation of E. coli O26, O111 and O157 from vegetables. Lett. Appl. Microbiol. 33 (2001) 36-39
16. Sharma V.K. Detection and quantitation of enterohemorrhagic E. coli O157, O111 and O26 in beef and bovine feces by real-time polymerase chain reaction. J. Food Protect. 65 (2002) 1371-1380
17. Tarr P.I., and Neill M.A. Perspective: the problem of non-O157 Shiga toxin (verocytotoxin)-producing E. coli. J. Infect. Dis. 174 (1996) 1136-1139
18. Ternent H.E., Innocent G.T., Filshie L.M., Taylor D.J., Steele W.B., McEwen S.A., Reilly W.J., Gunn G.J., Reid S.W.J., and Mellor D.J. Frozen storage of E. coli O157 in buffered peptone water and its detection on bovine carcasses. J. Food Protect. 67 (2004) 40-45