Comparison of the genetic effects of equimolar doses of ENU and MNU: While the chemicals differ dramatically in their mutagenicity in stem-cell spermatogonia, both elicit very high mutation rates in differentiating spermatogonia

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 616, Issue 1-2, 2007, Pages 181-195

  Russell, Liane B ^a   Hunsicker, Patricia R ^a   Russell, William L ^a  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

Author keywords

Ethylnitrosourea (ENU); Germ cell stage; Methylnitrosourea (MNU); Mouse; Mutagenesis; Specific locus mutations; Spermatogonia

Indexed keywords

ETHYLNITROSOUREA; METHYLNITROSOUREA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CHROMOSOME ABERRATION; CONTROLLED STUDY; DOSE RESPONSE; MALE; MEIOSIS; METAPHASE; MOSAICISM; MOUSE; MUTAGENICITY; MUTATION RATE; NONHUMAN; PRIORITY JOURNAL; REPRODUCTIVE TOXICITY; SPERMATID; SPERMATOGENESIS; SPERMATOGONIUM; STATISTICAL ANALYSIS; STEM CELL; SURVIVAL; TERATOGENICITY; WILD TYPE;

ALKYLATING AGENTS; ANIMALS; ETHYLNITROSOUREA; GERM-LINE MUTATION; MALE; METHYLNITROSOUREA; MICE; MICE, INBRED C3H; MUTAGENICITY TESTS; MUTAGENS; MUTATION; SPERMATOGENESIS; SPERMATOGONIA; STEM CELLS;

ANIMALIA;

EID: 33846690097     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrfmmm.2006.11.003     Document Type: Article

References (46)

1. Russell W.L., Kelly E.M., Hunsicker P.R., Bangham J.W., Maddux S.C., and Phipps E.L. Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse. Proc. Natl. Acad. Sci. U.S.A. 76 (1979) 5818-5819
2. Russell W.L., Hunsicker P.R., Raymer G.D., Steele M.H., Stelzner K.F., and Thompson H.M. Dose-response curve for ethylnitrosourea-induced specific-locus mutations in mouse spermatogonia. Proc. Natl. Acad. Sci. U.S.A. 79 (1982) 3589-3591
3. Hitotsumachi S., Carpenter D.A., and Russell W.L. Dose-repetition increases the mutagenic effectiveness of N-ethyl-N-nitrosourea in mouse spermatogonia. Proc. Natl. Acad. Sci. U.S.A. 82 (1985) 6619-6621
4. Marker P.C., Seung K., Bland A.E., Russell L.B., and Kingsley D.M. Spectrum of Bmp5 mutations from germ-line mutagenesis experiments in mice. Genetics 145 (1997) 435-443
5. Favor J. Mechanisms of mutation induction in germ cells of the mouse as assessed by the specific locus test. Mutat. Res. 428 (1999) 227-236
6. Huang J.-D., Cope M.J.T.V., Mermall V., Strobel M.C., Kendrick-Jones J., Russell L.B., Mooseker M.S., Copeland N.G., and Jenkins N.A. Molecular genetic dissection of unconventional myosin-VA: head region mutations. Genetics 148 (1998) 1951-1961
7. Huang J.-D., Mermall V., Strobel M.C., Russell L.B., Mooseker M.S., Copeland N.G., and Jenkins N.A. Molecular genetic dissection of unconventional myosin-VA: tail region mutations. Genetics 148 (1998) 1963-1972
8. Miltenberger R.J., Wakamatsu K., Ito S., Woychik R.P., Russell L.B., and Michaud E.J. Molecular and phenotypic analysis of 25 recessive, homozygous-viable alleles at the mouse agouti locus. Genetics 160 (2002) 659-674
9. Justice M. From the atomic age to the genome project. Genetica 122 (2004) 3-7
10. Russell W.L., and Hunsicker P.R. Extreme sensitivity of one particular germ-cell stage in male mice to induction of specific-locus mutations by methylnitrosourea. Environ. Mutagen. 5 (1983) 498
11. Russell W.L. Factors affecting mutagenicity of ethylnitrosourea in the mouse specific-locus test and their bearing on risk estimation. In: Sugimura T., Kondo S., and Takebe K. (Eds). Environmental Mutagens and Carcinogens (1982), University Tokyo Press/Alan R. Liss Inc., Tokyo, New York 59-70
12. Russell W.L., and Hunsicker P.R. Mutagenic effect of ethylnitrosourea (ENU) on poststem-cell stages in male mice. Environ. Mutagen. 6 (1984) 390
13. Favor J., Sund M., Neuhäuser-Klaus A., and Ehling U.H. A dose-response analysis of ethylnitrosourea-induced recessive specific-locus mutations in treated spermatogonia of the mouse. Mutat. Res. 231 (1990) 47-54
14. Favor J., Neuhäuser-Klaus A., and Ehling U.H. The frequency of dominant ctaract and recessive specific-locus mutation mosaics in F1 mice derived from port-spermatogonial treatment with ethylnitrosourea. Mutat. Res. 229 (1990) 105-114
15. Ehling U.H., and Neuhäuser-Klaus A. Induction of specific-locus and dominant lethal mutations in male mice by 1-methyl-1-nitrosourea (MNU). Mutat. Res. 250 (1991) 447-456
16. Russell W.L., Hunsicker P.R., Carpenter D.A., Cornett C.V., and Guinn G.M. Effect of dose fractionation on the ethylnitrosourea induction of specific-locus mutations in mouse spermatogonia. Proc. Natl. Acad. Sci. U.S.A. 79 (1982) 3592-3593
17. Russell W.L. X-ray-induced mutations in mice. Cold Spring Harbor Symp. Quant. Biol. 16 (1951) 327-336
18. Crow E.L., and Gardner R.S. Confidence intervals for the expectation of a Poisson variable. Biometrika 46 (1959) 441-453
19. Oakberg E.F. Germ cell toxicity: significance in genetic and fertility effects of radiation and chemicals. Environ. Sci. Res. 31 (1984) 549-590
20. Douglas G.R., Jiao J., Gingerich J.D., Gossen J.A., and Soper L.M. Temporal and molecular characteristics of mutations induced by ethylnitrosourea in germ cells isolated from seminiferous tubules and in spermatozoa of lacZ transgenic mice. Proc. Natl. Acad. Sci. U.S.A. 92 (1995) 7485-7489
21. Oakberg E.F., and Crosthwait C.D. The effect of ethyl-, methyl-, and hydroxyethyl-nitrosourea on the mouse testis. Mutat. Res. 108 (1983) 337-344
22. Generoso W.M., Cain K.T., Cornett C.C., and Cacheiro N.L.A. DNA target sites associated with chemical induction of dominant-lethal mutations and heritable translocations in mice. In: Chopra V.L., Joshi B.C., Sharma R.P., and Bansal H.C. (Eds). Frontiers in Genetics vol. 1 (1984), Oxford and IBH Publishing Co., New Delhi 347-356
23. Russell L.B., and Russell W.L. Spontaneous mutations recovered as mosaics in the mouse specific-locus test. Proc. Natl. Acad. Sci. U.S.A. 93 (1996) 13072-13077 (brief correction added in Proc. Natl. Acad. Sci. U.S.A. 94 (1997) 4233)
24. Russell L.B. Significance of the perigametic interval as a major source of spontaneous mutations that result in mosaics. Environ. Mol. Mutagen. 34 (1999) 16-23
25. van Delft J.H.M., Bergmans A., and Baan R.A. Germ-cell mutagenesis in llacZ transgenic mice treated with ethylating and methylating agents: comparison with specific-locus test. Mutat. Res. 388 (1997) 165-173
26. Nagao T. Frequency of congenital defects and dominant lethals in the offspring of mice treated with methylnitrosourea. Mutat. Res. 177 (1987) 171-178
27. Russell L.B. Effects of male germ-cell stage on the frequency, nature, and spectrum of induced specific-locus mutations in the mouse. Genetica 122 (2004) 25-36
28. Bultman S., Russell L.B., Gutierrez-Espeleta G.A., and Woychik R.P. Molecular characterization of a region of DNA associated with mutations at the agouti locus in the mouse. Proc. Natl. Acad. Sci. U.S.A. 88 (1991) 8062-8066
29. Smyth I.M., Wilming L., et al. Genomic anatomy of the Tyrp1 (brown) deletion complex. Proc. Natl. Acad. Sci. U.S.A. 103 (2006) 3704-3709
30. Rinchik E.M., Stoye J.P., Frankel W.N., Coffin J., Kwon B.S., and Russell L.B. Molecular analysis of viable spontaneous and radiation-induced albino (c)-locus mutations in the mouse. Mutat. Res. 286 (1993) 199-207
31. Johnson D.K., Stubbs L.J., Culiat C.T., Montgomery C.S., Russell L.B., and Rinchik E.M. Molecular analysis of 36 mutations at the mouse pink-eyed dilution (p) locus. Genetics 141 (1995) 1563-1571
32. Roix J.J., Hagge-Greenberg A., Bissonnette D.M., Rodick S., Russell L.B., and O'Brien T.P. Molecular and functional mapping of the piebald deletion complex on mouse chromosome 14. Genetics 157 (2001) 803-815
33. DiLeone R.J., Russell L.B., and Kingsley D.M. An extensive 3′ regulatory region controls expression of Bmp5 in specific anatomical structures of the mouse embryo. Genetics 148 (1998) 401-408
34. Russell L.B., and Montgomery C.S. Supermutagenicity of ethylnitrosourea in the mouse spot test; comparisons with methylnitrosourea and ethylnitrosourethane. Mutat. Res. 92 (1982) 193-204
35. L.B. Russell, W.L. Russell, E.M. Rinchik, P.R. Hunsicker, Factors affecting the nature of induced mutations, in: J.W., Allen, B.A., Bridges, M.F., Lyon, M.J., Moses, L.B., Russell (Eds.), Banbury Report 34: Biology of Mammalian Germ Cell Mutagenesis, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1990, pp 271-289.
36. Russell L.B., and Rinchik E.M. Structural differences between specific-locus mutations induced by different exposure regimes in mouse spermatogonial stem cells. Mutat. Res. 288 (1993) 187-195
37. Singer B. Mutagenesis from a chemical perspective: nucleic acid reactions, repair, translation, and transcription. In: Lemontt J.F., and Generoso W.M. (Eds). Basic Life Sciences, Molecular and Cellular Mechanisms of Mutagenesis vol. 20 (1982), Plenum Press, New York 1-42
38. 6-methylguanine-DNA methyltransferase in cell survival, mutagenesis and carcinogenesis (review). Mutat. Res. 145 (1985) 1-16
39. Provost G.C., and Short J.M. Characterization of mutations induced by ethylnitrososurea in seminiferous tubule germ cells of transgenic B6C3FI mice. Proc. Natl. Acad. Sci. U.S.A. 91 (1994) 6564-6568
40. Nivard M.J., Pastink A., and Vogel E.W. Mutational spectra induced under distinct excision repair conditions by the three methylating agents N-methyl-N-nitrosourea, N-methyl-N′-nitro-N-nitrosoguanidine, and N-nitrosodimethylamine in postmeiotic male germ cells of Drosophila. Mutat. Res. 10 (1996) 97-115
41. Generoso W.M. Relationship between alkylation sites and induction of dominant lethals and heritable translocations in mice. In: Ramel C., Lambert B., and Magnusson J. (Eds). Genetic Toxicology of Environmental Chemicals, Part B. Genetic Effects and Applied Mutagenesis (1986), Alan R. Liss Inc., New York 493-500
42. Generoso W.M., Cain K.T., Krishna M., and Huff S.W. Genetic lesions induced by chemicals in spermatozoa and spermatids of mice are repaired in the egg. Proc. Natl. Acad. Sci. U.S.A. 76 (1979) 435-437
43. Sotomayor R.E., Ehling U.H., and Sega G.A. Differential sensitivities of spermatogonial and postspermatogonial cell stages of the mouse to induction of unscheduled DNA synthesis by ethyl- and methyl-nitrosourea. Tertogen. Carcinogen. Mutagen. 19 (1999) 339-351
44. Riley B.B., and Grunwald D.J. Efficient induction of point mutations allowing recovery of specific locus mutations in zebrafish. Proc. Natl. Acad. Sci. U.S.A. 92 (1995) 5997-6001
45. Yoshikawa I., Avaki T., and Ohshima K. Comparative studies of dose-response curves for recessive lethal mutations induced by ethylnitrosourea in spermatogonia and spermatozoa of Drosophila melanogaster. Environ. Mutagen. 6 (1984) 489-496
46. Russell L.B., Bangham J.W., Stelzner K.F., and Hunsicker P.R. High frequency of mosaic mutants produced by N-ethyl-N-nitrosourea exposure of mouse zygotes. Proc. Natl. Acad. Sci. U.S.A. 85 (1988) 9167-9170