Translation control of trpG from transcripts originating from the folate operon promoter of Bacillus subtilis is influenced by translation-mediated displacement of bound TRAP, while translation control of transcripts originating from a newly identified trpG promoter is not

Journal of Bacteriology

Volumn 189, Issue 3, 2007, Pages 872-879

  Yakhnin, Helen ^a   Yakhnin, Alexander V ^a   Babitzke, Paul ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; FOLIC ACID; GENE PRODUCT; GLUTAMINE AMIDOTRANSFERASE; GLUTAMINE DERIVATIVE; RNA BINDING PROTEIN; TRANSFERASE; TRYPTOPHAN; TRYPTOPHAN RNA BINDING ATTENUATION PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BINDING AFFINITY; BINDING SITE; CISTRON; ENZYME SUBUNIT; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC CODE; GENETIC TRANSCRIPTION; INHIBITION KINETICS; NONHUMAN; NUCLEOTIDE REPEAT; PABB GENE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SYNTHESIS; RNA BINDING; SEQUENCE ANALYSIS; STOP CODON; TRANSLATION REGULATION; TRPG GENE; TRPP GENE; YCBK GENE;

ANTHRANILATE SYNTHASE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BASE SEQUENCE; ELECTROPHORETIC MOBILITY SHIFT ASSAY; FOLIC ACID; GENE EXPRESSION REGULATION, BACTERIAL; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; NITROGENOUS GROUP TRANSFERASES; OPERON; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PROTEIN BIOSYNTHESIS; RNA-BINDING PROTEINS; SEQUENCE ALIGNMENT; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

BACILLUS SUBTILIS;

EID: 33846639175     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.01398-06     Document Type: Article

References (32)

1. Antson, A. A., E. J. Dodson, G. Dodson, R. B. Greaves, X.-P. Chen, and P. Gollnick. 1999. Structure of the trp RNA-binding attenuation protein, TRAP, bound to RNA. Nature 401:235-242.
2. Antson, A. A., J. B. Otridge, A. M. Brzozowski, E. J. Dodson, G. G. Dodson, K. S. Wilson, T. M. Smith, M. Yang, T. Kurecki, and P. Gollnick. 1995. The three dimensional structure of trp RNA-binding attenuation protein. Nature 374:693-700.
3. Babitzke, P. 2004. Regulation of transcription attenuation and translation initiation by allosteric control of an RNA binding protein: the Bacillus subtilis TRAP protein. Curr. Opin. Microbiol. 7:132-139.
4. Babitzke, P., and C. Yanofsky. 1993. Reconstitution of Bacillus subtilis trp attenuation in vitro with TRAP, the trp RNA-binding attenuation protein. Proc. Natl. Acad. Sci. USA 90:133-137.
5. Babitzke, P., D. G. Bear, and C. Yanofsky. 1995. TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis, is a toroid-shaped molecule that binds transcripts containing GAG or UAG repeats separated by two nucleotides. Proc. Natl. Acad. Sci. USA 92:7916-7920.
6. Babitzke, P., J. T. Stults, S. J. Shire, and C. Yanofsky. 1994. TRAP, the trp RNA-binding attenuation protein of Bacillus subtilis, is a multisubunit complex that appears to recognize GAJAG repeats in the trpEDCFBA and trpG transcripts. J. Biol. Chem. 269:16597-16604.
7. Baumann, C., S. Xirsagar, and P. Gollnick. 1997. The trp RNA-binding attenuation protein (TRAP) from Bacillus subtilis binds to unstacked trp leader RNA. J. Biol. Chem. 272:19863-19869.
8. Deikus, G., P. Babitzke, and D. H. Bechhofer. 2004. Recycling of an RNA-binding protein by ribonuclease digestion. Proc. Natl. Acad. Sci. USA 101: 2747-2751.
9. de Saizieu, A., P. Vankan, C. Vockler, and A. P. G. M. van Loon. 1997. The trp RNA-binding attenuation protein (TRAP) regulates the steady-state levels of transcripts of the Bacillus subtilis folate operon. Microbiology 143: 979-989.
10. Dostie, J., and G. Dreyfuss. 2002. Translation is required to remove Y14 from mRNAs in the cytoplasm. Curr. Biol. 12:1060-1067.
11. Du, H., and P. Babitzke. 1998. trp RNA-binding attenuation protein-mediated long distance RNA refolding regulates translation of trpE in Bacillus subtilis. J. Biol. Chem. 273:20494-20503.
12. Du, H., R. Tarpey, and P. Babitzke. 1997. The trp RNA-binding attenuation protein regulates TrpG synthesis by binding to the trpG ribosome binding site of Bacillus subtilis. J. Bacteriol. 179:2582-2586.
13. Gollnick, P., P. Babitzke, A. Antson, and C. Yanofsky. 2005. Complexity in regulation of tryptophan biosynthesis in Bacillus subtilis. Annu. Rev. Genet. 39:47-68.
14. Gollnick, P., S. Ishino, M. I. Kuroda, D. J. Henner, and C. Yanofsky. 1990. The mtr locus is a two gene operon required for transcription attenuation in the trp operon of Bacillus subtilis. Proc. Natl. Acad. Sci. USA 87:8726-8730.
15. Gutierrez-Preciado, A., R. A. Jansen, C. Yanofsky, and E. Merino. 2005. New insights into regulation of the tryptophan biosynthetic operon in gram-positive bacteria. Trends Genet. 21:432-436.
16. Lu, J.-Y., N. Bergman, N. Sadri, and R. J. Schneider. 2006. Assembly of AUF1 with eIF4G-poly(A) binding protein complex suggests a translation function in AU-rich mRNA decay. RNA 12:883-893.
17. Lykke-Andersen, J., M.-D. Shu, and J. A. Steitz. 2000. Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon. Cell 103:1121-1131.
18. Merino, E., P. Babitzke, and C. Yanofsky. 1995. trp RNA-binding attenuation protein (TRAP)-trp leader RNA interactions mediate translational as well as transcriptional regulation of the Bacillus subtilis trp operon. J. Bacteriol. 177:6362-6370.
19. Otridge, J., and P. Gollnick. 1993. MtrB from Bacillus subtilis binds specifically to trp leader RNA in a tryptophan dependent manner. Proc. Natl. Acad. Sci. USA 90:128-132.
20. Platt, T., B. Müller-Hill, and J. H. Miller. 1972. Assay of β-galactosidase. In J. H. Miller (ed.), Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
21. Qi, Y., and F. M. Hulett. 1998. Role of Pho-P in transcriptional regulation of genes involved in cell wall anionic polymer biosynthesis in Bacillus subtilis. Mol. Microbiol. 28:1187-1197.
22. Sarsero, J. P., E. Merino, and C. Yanofsky. 2000. A Bacillus subtilis gene of previously unknown function, yhaG, is translationally regulated by tryptophan-activated TRAP and appears to be concerned with tryptophan transport. J. Bacteriol. 182:2329-2331.
23. Sarsero, J. P., E. Merino, and C. Yanofsky. 2000. A Bacillus subtilis operon containing genes of unknown function senses tRNAtrp charging and regulates expression of the genes for tryptophan biosynthesis. Proc. Natl. Acad. Sci. USA 97:2656-2661.
24. 2+-dependent RNA tertiary structure forms in the Bacillus subtilis trp operon leader transcript and appears to interfere with trpE translation control by inhibiting TRAP binding. J. Mol. Biol. 332:555-574.
25. Valbuzzi, A., P. Gollnick, P. Babitzke, and C. Yanofsky. 2002. The anti-trp RNA-binding attenuation protein (anti-TRAP), AT, recognizes the tryptophan-activated RNA binding domain of the TRAP regulatory protein. J. Biol. Chem. 277:10608-10613.
26. Yakhnin, A. V., and P. Babitzke. 2002. NusA-stimulated RNA polymerase pausing and termination participates in the Bacillus subtilis trp operon attenuation mechanism in vitro. Proc. Natl. Acad. Sci. USA 99:11067-11072.
27. Yakhnin, A. V., J. J. Trimble, C. R. Chiaro, and P. Babitzke. 2000. Effects of mutations in the L-tryptophan binding pocket of the trp RNA-binding attenuation protein of Bacillus subtilis. J. Biol. Chem. 275:4519-4524.
28. Yakhnin, H., A. V. Yakhnin, and P. Babitzke. 2006. The trp RNA-binding attenuation protein (TRAP) of Bacillus subtilis regulates translation initiation of ycbK, a gene encoding a putative efflux protein, by blocking ribosome binding. Mol. Microbiol. 61:1252-1266.
29. Yakhnin, H., H. Zhang, A. V. Yakhnin, and P. Babitzke. 2004. The trp RNA-binding attenuation protein (TRAP) of Bacillus subtilis regulates translation of the tryptophan transport gene, trpP (yhaG), by blocking ribosome binding. J. Bacteriol. 186:278-286.
30. Yakhnin, H., J. E. Babiarz, A. V. Yakhnin, and P. Babitzke. 2001. Expression of the Bacillus subtilis trpEDCFBA operon is influenced by translational coupling and Rho termination factor. J. Bacteriol. 183:5918-5926.
31. Yang, M., A. de Saizieu, A. P. G. M. Van Loon, and P. Gollnick. 1995. Translation of trpG in Bacillus subtilis is regulated by the trp RNA-binding attenuation protein (TRAP). J. Bacteriol. 177:4272-4278.
32. Yang, M., X.-P. Chen, K. Militello, R. Hoffman, B. Fernandez, C. Baumann, and P. Gollnick. 1997. Alanine-scanning mutagenesis of Bacillus subtilis trp RNA-binding attenuation protein (TRAP) reveals residues involved in tryptophan binding and RNA binding. J. Mol. Biol. 270:696-710.