A second galacturonic acid transferase is required for core lipopoylsaccharide biosynthesis and complete capsule association with the cell surface in Klebsiella pneumoniae

Journal of Bacteriology

Volumn 189, Issue 3, 2007, Pages 1128-1137

  Fresno, Sandra ^a   Jiménez, Natalia ^a   Canals, Rocío ^a   Merino, Susana ^a   Corsaro, Maria Michela ^b   Lanzetta, Rosa ^b   Parrilli, Michelangelo ^b   Pieretti, Giuseppina ^b   Regué, Miguel ^a   Tomás, Juan M ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; BACTERIUM LIPOPOLYSACCHARIDE; GALACTURONIC ACID; GALACTURONIC ACID TRANSFERASE; HEPTOSE; PROTEIN WABO; TRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL MEMBRANE; BACTERIAL PNEUMONIA; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CELL SURFACE; EXPERIMENTAL INFECTION; GENE MUTATION; IN VITRO STUDY; KLEBSIELLA PNEUMONIAE; LIPID ANALYSIS; LIPOGENESIS; NONHUMAN; PRIORITY JOURNAL; WAAQ GENE; WABO GENE;

ANIMALS; BACTERIAL CAPSULES; CARBOHYDRATE SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENES, BACTERIAL; GLYCOSYLTRANSFERASES; HEXURONIC ACIDS; KLEBSIELLA INFECTIONS; KLEBSIELLA PNEUMONIAE; LIPOPOLYSACCHARIDES; MAGNETIC RESONANCE SPECTROSCOPY; MICE; MICROSCOPY, IMMUNOELECTRON; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; VIRULENCE;

KLEBSIELLA; KLEBSIELLA PNEUMONIAE;

EID: 33846605211     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.01489-06     Document Type: Article

References (28)

1. Ciucanu, I., and F. Kerek. 1984. A simple and rapid method for the permethylation of carbohydrates. Carbohydr. Res. 131:209-217.
2. Climent, N., S. Ferrer, X. Rubires, S. Merino, J. M. Tomás, and M. Regué. 1997. Molecular characterization of a 17-kDa outer-membrane protein from Klebsiella pneumoniae. Res. Microbiol. 148:133-143.
3. Cortés, G, N. Borrell, B. Aztorza, C. Gómez, J. Sauleda, and S. Albertí. 2002. Molecular analysis of the contribution of the capsular polysaccharide and the lipopolysaccharide O side chain to the virulence of Klebsiella pneumoniae in a murine model of pneumonia. Infect. Immun. 70:2583-2590.
4. Emori, T. G., and R. P. Gaynes. 1993. An overview of nosocomial infections, including the role of the microbiology laboratory. Clin. Microbiol. Rev. 6:428-442.
5. Fresno, S., N. Jiménez, L. Izquierdo, S. Merino, M. M. Corsaro, C. De Castro, M. Parrilli, T. Naldi, M. Regué, and J. M. Tomás. 2006. The ioinic interaction of Klebsiella pneumoniae K2 capsule and core lipopolysaccharide. Microbiology 152:1807-1818.
6. Frirdich, E., E. Vinogradov, and C. Whitfield. 2004. Biosynthesis of a novel 3-deoxy-D-manno-oct-2-ulosonic acid-containing outer core oligosaccharide in the lipopolysaccharide of Klebsiella pneumoniae. J. Biol. Chem. 279:27928-27940.
7. Galanos, C., O. Lüderitz, and O. Westphal. 1969. A new method for the extraction of R lipopolysaccharides. Eur. J. Biochem. 9:245-249.
8. Hansen, D. S., F. Mestre, S. Albertí, S. Hernández- Alles, D. Alvarez, A. Domenech-Sánchez, J. Gil, S. Merino, J. M. Tomás, and V. J. Benedí. 1999. Klebsiella pneumoniae lipopolysaccharide O typing: revision of prototype strains and O-group distribution among clinical isolates from different sources and countries. J. Clin. Microbiol. 37:56-62.
9. Hitchcock, P. J., and T. M. Brown. 1983. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J. Bacteriol. 154:269-277.
10. Holst, O. 2002. Chemical structure of the core region of lipopolysaccharides-an update. Trends Glycosci. Glycotech. 14:87-103.
11. Izquierdo, L., N. Abitiu, N. Coderch, B. Hita, S. Merino, R. Gavín, J. M. Tomás, and M. Regué. 2002. The inner-core lipopolysaccharide biosynthetic waaE gene: function and genetic distribution among some Enterobacteriaceae. Microbiology 148:3485-3496.
12. Izquierdo, L., N. Coderch, N. Piqué, E. Bedini, M. Corsaro, S. Merino, S. Fresno, J. M. Tomas, and M. Regué. 2003. The Klebsiella pneumoniae wabG gene: its role in the biosynthesis of the core lipopolysaccharide and virulence. J. Bacteriol. 185:7213-7221.
13. Izquierdo, L., S. Merino, N. Coderch, M. Regué, and J. M. Tomás. 2002. The wavB gene of Vibrio cholerae and the waaE of Klebsiella pneumoniae codify for a beta-1,4- glucosyltransferase involved in the transfer of a glucose residue to the L-glycero-D-manno-heptose I in the lipopolysaccharide inner core. FEMS Microbiol. Lett. 216:211-216.
14. Link, A. J., D. Phillips, and G. M. Church. 1997. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179:6228-6237.
15. Marchler-Bauer, A., J. B. Anderson, P. F. Cherukuri, C. DeWeese-Scott, L. Y. Geer, M. Gwadz, S. He, D. I. Hurwitz, J. D. Jackson, Z. Ke, C. J. Lanczycki, C. A. Liebert, C. Liu, F. Lu, G. H. Marchler, M. Mullokandov, B. A. Shoemaker, V. Simonyan, J. S. Song, P. A. Thiessen, R. A. Yamashita, J. J. Yin, D. Zhang, and S. H. Bryant. 2005. CDD: a conserved domain database for protein classification. Nucleic Acids Res. 33:D192-D196.
16. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
17. Nassif, X., J. M. Fournier, J. Arondel, and P. J. Sansonetti. 1989. Mucoid phenotype of Klebsiella pneumoniae is a plasmid-encoded virulence factor. Infect. Immun. 57:546-552.
18. Orskov, I., and F. Orskov. 1984. Serotyping of Klebsiella. Methods Microbiol. 14:143-164.
19. Pradel, E., and C. A. Schnaitman. 1991. Effect of rfaH (sfrB) and temperature on expression of rfa genes of Escherichia coli K-12. J. Bacteriol. 173: 6428-6431.
20. Reed, L. J., and C. H. Muench. 1938. A simple method of estimating fifty percent end points. Am. J. Hyg. 27:493-497.
21. Regué, M., N. Climent, N. Abitiu, N. Coderch, S. Merino, L. Izquierdo, M. Altarriba, and J. M. Tomás. 2001. Genetic characterization of the Klebsiella pneumoniae waa gene cluster, involved in core lipopolysaccharide biosynthesis. J. Bacteriol. 183:3564-3573.
22. Regué, M., L. Izquierdo, S. Fresno, N. Piqué, M. M. Corsaro, T. Naldi, C. de Castro, D. Waidelich, S. Merino, and J. M. Tomás. 2005. A second outercore region in Klebsiella pneumoniae lipopolysaccharide. J. Bacteriol. 187: 4198-4206.
23. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Tsai, C. M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:115-119.
25. Vinogradov, E., M. Cedzynski, A. Ziolkowski, and A. Swierzko. 2001. The structure of the core region of the lipopolysaccharide from Klebsiella pneumoniae O3. 3-deoxy-α-D-manno-octulosonic acid (α-Kdo) residue in the outer part of the core, a common structural element of Klebsiella pneumoniae O1, O2, O3, O4, O5, O8, and O12 lipopolysaccharides. Eur. J. Biochem. 268:1722-1729.
26. Vinogradov, E., and M. B. Perry. 2001. Structural analysis of the core region of the lipopolysaccharides from eight serotypes of Klebsiella pneumoniae. Carbohydr. Res. 335:291-296.
27. Volk, V. A., N. L. Salomonsky, and D. Hunt. 1972. Xanthomonas sinensis cell wall lipopolysaccharide. I. Isolation of 4,7-anhydro- and 4,8-anhydro-3-deoxy-octulosonic acid following acid hydrolysis of Xanthomonas sinensis lipopolysaccharide. J. Biol. Chem. 247:3881-3887.
28. Westphal, O., and K. Jann. 1965. Bacterial lipopolysaccharide extraction with phenol-water and further application of the procedure. Methods Carbohydr. Chem. 5:83-91.