High efficiency and throughput system in directed evolution in vitro of reporter gene

Applied Microbiology and Biotechnology

Volumn 74, Issue 1, 2007, Pages 160-168

  Xiong, Ai Sheng ^a,c   Peng, Ri He ^a   Liu, Jin Ge ^a,c   Zhuang, Jing ^a,c   Qiao, Yu Shan ^c   Xu, Fang ^a,c   Cai, Bing ^a,c   Zhang, Zhen ^c   Chen, Jian Min ^b   Yao, Quan Hong ^a  

^a INSTITUTE OF EDIBLE FUNGI   (China)

^b YANGZHOU UNIVERSITY   (China)

^c NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Glucuronidase; DNA directed evolution; Gene shuffling; GUS reporter gene; Thermostability

Indexed keywords

DNA; ENZYME KINETICS; ENZYMES; ESCHERICHIA COLI; MATHEMATICAL MODELS; THERMODYNAMIC STABILITY;

GENE SHUFFLING; MUTATION RATES; PROKARYOTIC EXPRESSION VECTORS; PROTEIN STRUCTURE;

GENES;

BETA GLUCURONIDASE; PYROXYLIN;

COLIFORM BACTERIUM; DNA; ENZYME; ENZYME ACTIVITY; EVOLUTION; GENE EXPRESSION; PROTEIN;

ARTICLE; BACTERIUM COLONY; DIALYSIS; DNA SEQUENCE; DNA SHUFFLING; ELECTROPHORESIS; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE LIBRARY; GENE MUTATION; GENETIC SCREENING; GENETIC TRANSCRIPTION; GENETIC VARIABILITY; HEATING; HIGH TEMPERATURE; IN VITRO STUDY; INCUBATION TIME; METHODOLOGY; MOLECULAR EVOLUTION; MUTATION RATE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROKARYOTE; PROTEIN PURIFICATION; REPORTER GENE; TRANSCRIPTION TERMINATION; WILD TYPE;

BIOTECHNOLOGY; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; ENZYME STABILITY; ESCHERICHIA COLI; GENES, BACTERIAL; GENES, REPORTER; GENETIC ENGINEERING; GLUCURONIDASE; HEAT; MOLECULAR SEQUENCE DATA; SEQUENCE ANALYSIS, DNA;

ESCHERICHIA COLI; PROKARYOTA;

EID: 33846602199     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0659-0     Document Type: Article

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