On the use of ratio standard curves to accurately quantitate relative changes in protein levels by Western blot

Analytical Biochemistry

Volumn 361, Issue 2, 2007, Pages 305-307

  Pitre, Aaron ^a   Pan, Yihang ^a   Pruett, Steve ^a   Skalli, Omar ^a  

^a LOUISIANA STATE UNIVERSITY HEALTH SCIENCES CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

DENSITOMETRIC; DENSITOMETRIC MEASUREMENT; DENSITOMETRY; PROTEIN EXPRESSIONS; PROTEIN LEVEL; RATIO STANDARD; SIMPLE METHOD; WESTERN BLOTS;

PROTEINS;

CELL PROTEIN; PROTEIN;

ARTICLE; CURVE FITTING; DENSITOMETRY; GEL; GENE EXPRESSION; GENETIC TRANSFECTION; LINEAR REGRESSION ANALYSIS; MATHEMATICAL ANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; STATISTICAL ANALYSIS; WESTERN BLOTTING;

EID: 33846542342     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.11.008     Document Type: Article

References (4)

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3. Towbin H., Staehelin T., and Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76 (1979) 4350-4354
4. Tinoco Jr. I., Sauer K., and Wang J.C. Physical Chemistry: Principles and Applications in Biological Sciences. 3rd ed. (1995), Prentice Hall, Englewood Cliffs, NJ