Isolation and characterization of four isoinhibitors from cowpea (Vigna unguiculata (L.) Walp.) seeds

Turkish Journal of Biology

Volumn 30, Issue 4, 2006, Pages 207-215

  Sammour, Reda Helmy Ahmed ^a  

^a Tanta University   (Egypt)

Author keywords

Cowpea trypsin inhibitor; CPTI; N benzoyl L arginine p nitroanilide (BAPNA); N benzoyl L tyrosine ethyl ester (BTEE); P nitrophenyl P guanidinobenzoate HCl (NPGB)

Indexed keywords

MACROTYLOMA AXILLARE; PHASEOLUS LUNATUS; VIGNA RADIATA VAR. RADIATA; VIGNA UNGUICULATA;

EID: 33846506520     PISSN: 13000152     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (46)

1. Ceciliani F, Tava A, Iori R et al. A trypsin inhibitor from snail medic seeds active against pest proteases. Phytochemistry 44: 393-398, 1997.
2. Ye XY, Ng TB, Rao PF. A Bowman-Birk-type trypsin-chymotrypsin inhibitor from broad beans. Biochem Biophys Res Commun. 289: 91-96, 2001.
3. Rodrigues Macedo ML, Machado Freire MG, Cabrini EC et al. A trypsin inhibitor from Peltophorum dubium seeds active against pest proteases and its effect on the survival of Anagasta kuehniella (Lepidoptera: Pyralidae). Biochim Biophys Acta 1621: 170-182, 2003.
4. Schaller A. A cut above the rest: the regulatory function of plant proteases. Planta 220: 183-97, 2004.
5. Xia Y. Proteases in pathogenesis and plant defense. Cell Microbiol 6: 905-913, 2004.
6. Mello GC, Oliva ML, Sumikawa JT et al. Purification and characterization of a new trypsin inhibitor from Dimorphandra mollis seeds. J Protein Chem 20: 625-32, 2001.
7. Silva JA, Macedo ML, Novello JC et al. Biochemical characterization and N-terminal sequences of two new trypsin inhibitors from Copaifera langsdorffii seeds. J Protein Chem 20: 1-7, 2001.
8. Haq SK, Khan RH. Characterization of a proteinase inhibitor from Cajanus cajan (L.). J Protein Chem 22: 543-54, 2003.
9. Giri AP, Harsulkar AM, Ku MS et al. Identification of potent inhibitors of Helicoverpa armigera gut proteinases from winged bean seeds. Phytochemistry. 63: 523-32, 2003.
10. Garcia VA, Freire MG, Novello JC et al. Trypsin inhibitor from Poecilanthe parviflora seeds: purification, characterization, and activity against pest proteases. Protein J 23: 343-50, 2004.
11. Weder JK, Hinkers SC. Complete amino acid sequence of the lentil trypsin-chymotrypsin inhibitor LCI-1.7 and a discussion of atypical binding sites of Bowman-Birk inhibitors. J Agric Food Chem 52: 4219-26, 2004
12. Borchers R, Ackerson CW, Kimmett L. Trypsin inhibitor (IV) occurrence in seeds of the Leguminosae and other seeds. Arch Biochem 13: 291-296, 1947.
13. Jaffe WG. Toxic factors in Leguminosae. Proc Soc Exp Biol Med 75: 219-225, 1950.
14. Ventura MM, Filho JX. Purification of trypsin inhibitors from Vigna sinesls. An. Acad. Bras Ceinc 39: 553-559, 1966.
15. Ventura MM, Filho JX, Moreira RA et al. Isolation and characterization of two trypsin inhibitors from Vigna sinesis. An Acad Bras Cienc 43: 233-238, 1971.
16. Gennis LS, Cantor CR. Double-headed protease inhibitors from black-eyed peas. 1. Purification of two new protease inhibitors and the endogenous protease by affinity chromatography. J Biol Chem 251: 734-740, 1976.
17. Gatehouse AMR, Gatehouse JA. Boulter D. Isolation and characterization of trypsin inhibitors from cowpea (Vigna unguiculata). Phytochemistr, 19: 751-756, 1980.
18. Schwertz GW, Takenaka Y. A spectrophotometric determination of trypsin and chymotrypsin activity. Biochim Biophys Acta 16: 571-575, 1955.
19. Suk-Hee K, Morton SF. An improvement in the hummel chymotrypsin assay. Anal Biochem 54 262-265, 1973.
20. Appel W. Amino acid aryl amidases (leucine-nitroanilidase). Determination with L-leucine p-nitroanilides substrates. In: Bergemeyer H ed. Methods of Enzymatic Analysis Vol 2. Verlag Chemie USA International, Deerfield Beach: 1974: pp. 958-963.
21. Hashimoto F, Horigome T, Kanbayashi M et al. An improved method for separation of low molecular weight polypeptides by electrophoresis in sodium dodecyl sulphate-polyacrylamide gel. Analytical Biochemistry 129: 192-199, 1983.
22. Vesterberg O.Isoelectric focusing of proteins. Meth Enzymol 22:389-412, 1971.
23. Glazer AN, Delange RJ, Sigman D S. Chemical modification of proteins - Selected Methods and Analytical Procedures. North-Holland/Elsevier, Amsterdam 1975.
24. Croy RR, Gatehouse JA, Tyler M, Boulter D. The purification and characterization of a third storage protein (convicilin) from the seeds of pea (Pisum sativum L.). Biochem J 191: 509-516, 1980.
25. Moore S. On the determination of cystine as cysteic acid. The Journal of Biological Chemistry 238: 235-237, 1963.
26. Gray WR. Sequence analysis with dansyl chloride. Methods Enzymol 25: 333-344, 1972.
27. Hirano H, Gatehouse J A, Boulter D. The complete amino acid sequence of a subunit of the vicilin seed storage protein of pea (Pisum sativum L.). FEBS LETTERS 145: 99-102, 1982.
28. Chang JY, Brauer D, Wittmann-Liebold B. Micro-sequence analysis of peptides and proteins using 4-NN-dimethylaminoazobenzene 4_-isothiocyanate/ phenylisothiocyanate double coupling method. FEBS LETTERS 93: 205-214, 1978.
29. Laskowski M, Jr. Sealock RW. Protein proteinase inhibitors-molecular aspects. Enzymes 3: 376-473, 1971.
30. Haynes R, Osuga DT, Feeney RF. Modification of amino groups in inhibitors of proteolytic enzymes. Biochemistry 6: 541-547, 1967.
31. Joubert FJ, Kruger H, Townshend G et al. Purification, some properties and complete primary structure of two protease inhibitors (DE-3 and DE-4) from Macrotyloma axillare seeds. Eur. J. Biochem 97: 85-91, 1979.
32. Yoshikawa M, Kiyohara, T, Iwasaki T et al. Amino acid sequences of proteinase inhibitors II and III from adzuki beans. Agric Biol Chem 43: 787-796, 1979.
33. Steven FC, Woery S, Krahu J. Structure-function relationship in lima bean proteinase inhibitor. In: Fritz H, Tschesche H, Greene LJ, Truscheit E. eds. Proteinase Inhibitors. Springer-Verlag. Berlin: pp. 344-354, 1974.
34. Wilson KA, Chen JC. Amino acid sequence of Mung bean trypsin inhibitor and its modified forms appearing during germination. Plant Physiol 2:341-349, 1983.
35. Haynes R, Feeney RF. Fractionation and properties of trypsin and chymotrypsin inhibitors from lima beans. J Biol Biochem 242: 5378- 5385, 1967.
36. Frattali V, Steiner RF. Interaction of trypsin and chymotrypsin with a soybean proteinase inhibitor. Biochem Biophys Res Commun 34: 480-487, 1969.
37. Wilson KA, Laskowski M Jr. The partial amino acid sequence of trypsin inhibitor Il from garden bean, Phaseolus vulgaris, with location of trypsin and elastase reactive sites. J Biol Chem 250: 4261-4267, 1975.
38. Kumar P, Rao AG, Hariharaputran S et al. Molecular mechanism of dimerization of Bowman-Birk inhibitors. Pivotal role of ASP76 in the dimerzation. J Biol Chem 279: 30425-30432, 2004.
39. Mikola J. Proteinases, peptidases, and inhibitors of endogenous proteinases in germinating seeds. In: Daussant J, Mosse J, Vaughan J. eds. Seed Proteins. Academic Press, London and New York: pp 35-52, 1983.
40. Ozawa K, Laskowski M. Jr. The reactive site of trypsin inhibitors. J Biol Chem 241: 3955-3961, 1966.
41. Belew M, Porath J, Sundberg L. The trypsin and chymotrypsin inhibitors in chick peas (Cicer arientinum L.) Purification and properties of the inhibitors. Eur J Biochem 60: 247-258, 1975.
42. Belew M, Eaker D. The trypsin and chymotrypsin inhibitors in chick peas (Cicer arientinum L.). Identification of the trypsin-reactive site, partial-amino-acid sequence and further physiochemical properties of the major inhibitor. Eur J Biochem 62: 499-508, 1976.
43. Krahn J, Stevens F. Lima bean trypsin inhibitor. Limited proteolysis by trypsin and chymotrypsin. Biochemistry 9: 2646-2652, 1970.
44. Feinstein G, Gertler A. Modification of essential carboxylic acid side chains of trypsin. Eur J Biochem 31: 25-31, 1972.
45. Odani S, Ikenaka T. Studies on soybean trypsin inhibitors IV. Complete amino acid sequence and antiproteinase site of Bowman-Birk proteinase anhibitor. J Biochem 71: 839-848, 1972.
46. Tan CGL, Stevens FC. Amino acid sequence of lima bean protease inhibitor component IV. 2. Isolation and sequence determination of the chymotryptic peptides and the complete amino acid sequence. Eur J Biochem 18: 515-523, 1971.