Proliferation of mycobacteria in a piggery environment revealed by mycobacterium-specific real-time quantitative PCR and 16S rRNA sandwich hybridization

Veterinary Microbiology

Volumn 120, Issue 1-2, 2007, Pages 105-112

  Pakarinen, J ^a   Nieminen, T ^b   Tirkkonen, T ^a   Tsitko, I ^a   Ali Vehmas, T ^a   Neubauer, P ^b   Salkinoja Salonen, M S ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b UNIVERSITY OF OULU   (Finland)

Author keywords

Mycobacteria; Pig mycobacteriosis; Piggery environment; Real time qPCR; Sandwich hybridization

Indexed keywords

BACTERIAL DNA; DYE; RNA 16S;

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM DETECTION; BED; CARCASS; CONTROLLED STUDY; DNA EXTRACTION; DNA PROBE; FINLAND; GENE AMPLIFICATION; HERD; MYCOBACTERIOSIS; MYCOBACTERIUM; NONHUMAN; PREVALENCE; REAL TIME POLYMERASE CHAIN REACTION; RNA HYBRIDIZATION; SWINE; TUBERCULOSIS;

ANIMAL HUSBANDRY; ANIMALS; BEDDING AND LINENS; FEMALE; MYCOBACTERIUM; MYCOBACTERIUM INFECTIONS; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SWINE; SWINE DISEASES;

ANIMALIA; CORYNEBACTERINEAE; MYCOBACTERIUM; SUIDAE; SUS;

EID: 33846463480     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2006.10.016     Document Type: Article

References (21)

1. Ali-Vehmas T., Moisander A.-M., and Soini H. Mycobacteriosis-a review and survey in Finland. Fin. Vet. J. 110 (2004) 79-84
2. Biet F., Boschiroli M.L., Thorel M.F., and Guilloteau L.A. Zoonotic aspects of Mycobacterium Bovis and Mycobacterium avium-intracellulare complex (MAC). Vet. Res. 36 (2005) 411-436
3. Cole J.R., Chai B., Marsh T.L., Farris R.J., Wang Q., Kulam S.A., Chandra S., McGarrell D.M., Schmidt T.M., Garrity G.M., and Tiedje J.M. The ribosomal database project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res. 31 (2003) 442-443
4. Falkinham J.O. Environmental Sources of Mycobacterium avium Linked to Routes of Exposure, from the World Health Organization Book Pathogenic Mycobacteria in Water: a Guide to Public Health Consequences Monitoring and Management (2004), IWA Publishing, London, UK pp. 26-37
5. Howard S.T., and Byrd T.F. The rapidly growing mycobacteria: saprophytes and parasites. Microbes Infect. 2 (2000) 1853-1854
6. Iivanainen E., Martikainen P.J., Räisänen M.L., and Katila M.L. Mycobacteria in boreal coniferous forest soils. FEMS Microbiol. Ecol. 23 (1997) 325-332
7. Khan I.U.H., and Yadav J.S. Development of a single-tube, cell lysis-based, genus-specific PCR method for rapid identification of mycobacteria: optimization of cell lysis, PCR primers and conditions, and restriction pattern analysis. J. Clin. Microbiol. 42 (2004) 453-457
8. Kirschner Jr. R.A., Parker B.C., and Falkinham III J.O. Epidemiology of infection by nontuberculous mycobacteria. Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum, in acid, brown-water swamps of the southeastern United States, association with environmental variables. Am. Rev. Respir. Dis. 145 (1992) 271-275
9. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F.-C., and Böttger E.C. Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J. Clin. Microbiol. 31 (1993) 2882-2889
10. Klappenbach J.A., Saxman P.R., Cole J.R., and Schmidt T.M. rrndb: the ribosomal RNA operon copy number database. Nucleic Acid Res. 29 (2001) 181-184
11. Komijn R.E., De Haas P.E., Schneider M.M.E., Eger T., Nieuwenhuijs J.H.M., Van Den Hoek J., Bakker D., Van Zijd Erveld F.G., and Van Soolingen D. Prevalence of Mycobacterium avium in slaughter pigs in the Netherlands and comparison of IS1245 restriction fragment length polymorphism patterns of porcine and human isolates. J. Clin. Microbiol. 37 (1999) 1254-1259
12. Matlova L., Dvorska L., Bartl J., Bartos M., Ayele W.Y., Alexa M., and Pavlik I. Mycobacteria isolated from the environment of pig farms in the Czech Republic during the years 1996 to 2002. Vet. Med. Czech. 48 (2003) 343-357
13. Matlova L., Dvorska L., Palecek K., Maurenc L., Bartos M., and Pavlik I. Impact of sawdust and wood shavings on pig bedding on pig tuberculous lesions in lymph nodes, and IS1245 RFLP analysis of Mycobacterium avium subsp. Hominissuis of serotypes 6 and 8 isolated from pigs and environment. Vet. Microbiol. 102 (2004) 227-236
14. Matlova L., Dvorska L., Ayele W.Y., Bartos M., Amemori T., and Pavlik I. Distribution of Mycobacterium avium complex isolates in tissue samples of pigs fed peat naturally contaminated with mycobacteria as a supplement. J. Clin. Microbiol. 43 (2005) 1261-1268
15. Mendum T.A., Chilima B.Z., and Hirsch P.R. The PCR amplification of non-tuberculous mycobacterial 16S rRNA sequences from soil. FEMS Microbiol. Lett. 185 (2000) 189-192
16. Nieminen T., Pakarinen J., Tsitko I., Salkinoja-Salonen M., Breitenstein A., Ali-Vehmas T., and Neubauer P. 16S rRNA targeted sandwich hybridization method for direct quantification of mycobacteria in soils. J. Microbiol. Meth. 67 (2006) 44-55
17. Primm T.P., Lucero C.A., and Falkinham III J.O. Health impacts of environmental mycobacteria. Appl. Environ. Microbiol. 17 (2004) 98-106
18. Ramasoota P., Chansiripornchai N., Källenius G., Hoffner S., and Svenson S.B. Comparison of Mycobacterium avium complex (MAC) strains from pigs and humans in Sweden by random amplified polymorphic DNA (RAPD) using standardized reagents. Vet. Microbiol. 12 (2001) 251-259
19. Talaat A.M., Reimschuessel R., and Trucksis M. Identification of mycobacteria to the species level using polymerase chain reaction and restriction enzyme analysis. Vet. Microbiol. 58 (1997) 221-237
20. Thorel M.F., Huchzermeyer H.F., and Michel A.L. Mycobacterium avium and Mycobacterium intracellulare infection in mammals. Rev. Sci. Tech. Off. Int. Epiz. 20 (2001) 204-218
21. Windsor R.S., Durrant D.S., and Burn K.J. Avian tuberculosis in pigs: Mycobacterium intracellulare infection in a breeding herd. Vet. Rec. 114 (1984) 497-500