Real-Time RT-PCR assay to quantify the expression of fum1 and fum19 genes from the Fumonisin-producing Fusarium verticillioides

Journal of Microbiological Methods

Volumn 68, Issue 2, 2007, Pages 312-317

  López Errasquín, Elena ^a   Vázquez, Covadonga ^a   Jiménez, Misericordia ^b   González Jaén, Ma Teresa ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b UNIVERSITY OF VALENCIA   (Spain)

Author keywords

fum1; fum19; Fumonisins; Fusarium verticillioides; Real time RT PCR

Indexed keywords

ABC TRANSPORTER; FUMONISIN; FUMONISIN B1; MYCOTOXIN; POLYKETIDE SYNTHASE;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; FOOD CONTAMINATION; FUM1 GENE; FUM19 GENE; FUNGAL DETECTION; FUSARIUM; FUSARIUM VERTICILLIOIDES; GENE; GENE EXPRESSION; GENE FUNCTION; GIBBERELLA FUJIKUROI; HEALTH HAZARD; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CULTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ATP-BINDING CASSETTE TRANSPORTERS; FUMONISINS; FUSARIUM; GENE EXPRESSION REGULATION, FUNGAL; POLYKETIDE SYNTHASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, FUNGAL;

ANIMALIA; FUSARIUM; GIBBERELLA FUJIKUROI; GIBBERELLA MONILIFORMIS;

EID: 33846428789     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2006.09.007     Document Type: Article

References (24)

1. Bezuidenhout S.C., Gelderblom W.C.A., Gorst-Allman C.P., Horak R.M., Marasas W.F.O., Spiteller G., and Vleggaar R. Structure elucidation of the fumonisins, mycotoxins from Fusarium moniliforme. J. Chem. Soc. 1988 (1988) 743-745
2. Bojja R.S., Cerny R.L., Proctor R.H., and Du L. Determining the biosynthetic sequence in the early steps of the fumonisin pathway by use of three gene disruption mutants of F. verticillioides. J. Agric. Food Chem. 52 (2004) 2855-2860
3. Covert S.F., Briley A., Wallace M.M., and Mckinney. V.T. Partial MAT-2 gene structure and the influence of temperature on mating success in Gibberella circinata. Fungal Genet. Biol. 28 (1999) 43-54
4. Desjardins A.E. Gibberella from A (venaceae) to Z (eae). Annu. Rev. Phytopathol. 41 (2003) 177-198
5. Doohan F.M., Weston G., Rezanoor H.N., Parry D.W., and Nicholson P. Development and use of a reverse transcription-PCR assay to study expression of Tri5 by Fusarium species in vitro and in planta. Appl. Environ. Microbiol. 65 (1999) 3850-3854
6. Gardiner D.M., Jarvis R.S., and Howlett B.J. The ABC transporter gene in the sirodesmin biosynthetic gene cluster of Leptosphaeria maculans is not essential for sirodesmin production but facilitates self-protection. Fungal Genet. Biol. 42 (2005) 257-263
7. Geisen R. Molecular monitoring of environmental conditions influencing the induction of ochratoxin A biosynthesis genes in Penicillium nordicum. Mol. Nutr. Food Res. 48 (2004) 532-540
8. Ginzinger D.G. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp. Hematol. 30 (2002) 503-512
9. Glass N.L., and Donaldson G.C. Development of primer sets designed for use with PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61 (1995) 1323-1330
10. González-Jaén M.T., Mirete S., Patiño B., López-Errasquín E., and Vázquez C. Genetic markers for the analysis of variability and for production of specific diagnostic sequences in fumonisin-producing strains of Fusarium verticillioides. Eur. J. Plant Pathol. 110 (2004) 525-532
11. Higgins C.F. ABC transporters: from microorganisms to man. Annu. Rev. Cell Biol. 8 (1992) 67-113
12. 1 carcinogenicity in a two-year feeding study using F344 rats and B6C3F1 mice. Environ. Health Perspect. suppl. 2 (2001) 277-282
13. Jiménez M., Mateo J.M., Hinojo M.J., and Mateo R. Sugars and amino-acids as factors affecting the synthesis of fumonisins in liquid cultures of isolates of the Gibberella fujikuroi complex. Int. J. Food Microbiol. 89 (2003) 185-193
14. Jurado M., Vázquez C., Patiño B., and González-Jaén M.T. PCR detection assays for the trichothecene-producing species Fusarium graminearum, Fusarium culmorum, Fusarium poae, Fusarium equiseti and Fusarium sporotrichioides. Syst. Appl. Microbiol. 28 (2005) 562-568
15. Keréngy Z., Zeller K., Hornok L., and Leslie J.F. Molecular standardization of Mating Type terminology in the Gibberella fujikuroi species complex. Appl. Environ. Microbiol. 65 (1999) 4071-4076
16. Leslie J.F. Gibberella fujikuroi: available populations and variable traits. Can. J. Bot. 73 (1995) 5282-5291
17. Mirete S., Vázquez C., Mulé G., Jurado M., and González-Jaén M.T. Differentiation of Fusarium verticillioides from banana fruits by IGS and EF-1α sequence analyses. Eur. J. Plant Pathol. 110 (2004) 515-523
18. Nelson P.E., Desjardins A.E., and Plattner R.D. Fumonisins, mycotoxins produced by Fusarium species: biology, chemistry, and significance. Annu. Rev. Phytopathol. 31 (1993) 233-252
19. Nicholson P., Chandler E., Draeger R.C., Gosman N.E., Simpson D.R., Thomsett M., and Wilson A.H. Molecular tools to study epidemiology and toxicology of Fusarium head blight of cereals. Eur. J. Plant Pathol. 109 (2003) 691-703
20. Patiño B., González-Salgado A., González-Jaén M.T., and Vázquez C. PCR detection assays for the ochratoxin-producing Aspergillus carbonarius and Aspergillus ochraceus species. Int. J. Food Microbiol. 104 (2005) 207-214
21. Proctor R.H., Desjardins A.E., Plattner R.D., and Hohn T.M. A polyketide synthase gene required for biosynthesis of fumonisin mycotoxins in Gibberella fujikuroi mating population A. Fungal Genet. Biol. 27 (1999) 100-112
22. Proctor R.H., Brown D.W., Plattner R.D., and Desjardins A.E. Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis. Fungal Genet. Biol. 38 (2003) 237-249
23. Waalwijk C., van der Heide R., de Vries I., van der Lee T., Schoen C., Costrel-de Corainville G., Häuser-Hahn I., Kastelein P., Köhl J., Lonnet P., Demarquet T., and Kema G.H.J. Major changes in Fusarium spp. in wheat in the Netherlands. Eur. J. Plant Pathol. 110 (2004) 481-494
24. Wang E., Norred W.P., Bacon C.W., Riley R.T., and Merrill A.H. Inhibition of sphingolipid biosynthesis by fumonisins: implications for diseases associated with Fusarium moniliforme. J. Biol. Chem. 266 (1991) 14486-14490