Cultured autologous keratinocytes in suspension accelerate epithelial maturation in an in vivo wound model as measured by surface electrical capacitance

Plastic and Reconstructive Surgery

Volumn 119, Issue 2, 2007, Pages 495-499

  Magnusson, Mark ^b   Papini, Remo P ^b   Rea, Suzzane M ^b   Reed, Chris C ^b   Wood, Fiona M ^a  

^a ROYAL PERTH HOSPITAL   (Australia)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; BURN; CELL MATURATION; CELL SUSPENSION; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DOUBLE BLIND PROCEDURE; ELECTRIC CAPACITANCE; FEMALE; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; KERATINOCYTE; MALE; POSTOPERATIVE PERIOD; PRIORITY JOURNAL; RANDOMIZATION; RANDOMIZED CONTROLLED TRIAL; SKIN AUTOGRAFT; SKIN CONDUCTANCE; SKIN EPITHELIUM; SKINFOLD THICKNESS; SPLIT THICKNESS SKIN GRAFT; STATISTICAL SIGNIFICANCE; WOUND HEALING;

ADULT; BURNS; CELLS, CULTURED; DOUBLE-BLIND METHOD; ELECTRIC CAPACITANCE; EPITHELIAL CELLS; EPITHELIUM; FEMALE; HUMANS; KERATINOCYTES; MALE; MIDDLE AGED; MODELS, BIOLOGICAL; SKIN TRANSPLANTATION; TRANSPLANTATION, AUTOLOGOUS; WOUND HEALING;

EID: 33846407229     PISSN: 00321052     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.prs.0000246315.80133.8d     Document Type: Article

References (20)

1. Jarret, A. The epidermis and its relationship with the dermis. In A. Jarrett (Ed.), The Physiology and Pathophysiology of the Skin. New York: Academic Press, 1972. Pp. 3-5.
2. Schwindt, D., Wilhelm, K. P., and Maibach, H. Water diffusion characteristics of human stratum corneum at different anatomical sites in vivo. J. Invest. Dermatol. 111: 385, 1998.
3. Berardesca, E., and Maibach, H. Racial differences in skin pathophysiology. J. Am. Acad. Dermatol. 34: 667, 1996.
4. Reed, J. T., Ghadially, R., and Elias, P. M. Skin type, but neither race nor gender, influence epidermal permeability barrier function. Arch. Dermatol. 131: 1134, 1995.
5. Yosipovitch, G., Xiong G. L., Haus, E., Sackett-Lundeen, L., Ashkenazi, I., and Maibach, H. Time dependent variations of the skin barrier function in humans: Transepidermal water loss, stratum corneum hydration, skin surface pH, and skin temperature. J. Invest. Dermatol. 110: 20, 1998.
6. Moyer, C. A., and Butcher, H. R. Burns, Shock, and Plasma Regulation. St. Louis: Mosby, 1967.
7. Jelenko, C. Studies in burns: VII. The water retaining lipids of eschars: Their presence and potential usefulness. Am. Surg. 5: 709, 1969.
8. Moncrief, J. A., and Mason, A. D. Water vapour loss in the burned patient. Surg. Forum 13: 38, 1962.
9. Harrison, H. N., Moncrief, J. A., and Duckett, J. W. The relationship between energy metabolism and water loss from vapourisation in severely burned patients. Surgery 56: 203, 1964.
10. Birke, G., Liljedahl, S. O., Olsson, K. E., and Plantin, L. O. Total exchange and insensible loss of water in severe burns during the first two weeks. Acta Chir. Scand. 136: 125, 1970.
11. Hammarlund, K., and Sedin, G. Transepidermal water loss in newborn infants: III. Relation to gestational age. Acta Paediatr. Scand. 68: 795, 1979.
12. Sedin, G., Hammarlund, K., Nilsson, G. E., Stromberg, B., and Oberg, P. A. Measurement of transepidermal water loss in newborn infants. Clin. Perinatol. 12: 79, 1985.
13. Leveque, J. L., and De Rigal, J. Impedance methods for studying skin moisturization. J. Soc. Cosmet. Chem. 29: 419, 1983.
14. Gabard, B., and Treffel, P. Hardware and measure principle: The Nova DPM 9003. In P. Elsner, E. Berardesca, and H. I. Maibach (Eds.), Bioengineering of the Skin: Water and the Stratum Corneum. Boca Raton, Fla.: CRC Press, 1994. P. 177.
15. Jemec, G. B., and Serup, J. Epidermal hydration and skin mechanics: The relationship between electrical capacitance and the mechanical properties of the human skin in vivo. Acta Derm. Venereol. 70: 245, 1990.
16. Boyce, S. T., Supp, A. P., Harriger, M. D., Pickens, W. L., Wickett, R. R., and Hoath, S. B. Surface electrical capacitance as a noninvasive index of epidermal barrier in cultured skin substitutes in athymic mice. J. Invest. Dermatol. 107: 82, 1996.
17. Goretsky, M. J., Supp, A. P., Greenhalgh, D. J., Warden, G. D., and Boyce, S. T. Surface electrical capacitance as an index of epidermal barrier properties of composite skin substitutes and skin autografts. Wound Repair Regen. 3: 419, 1995.
18. Fratianne, R., Papay, F., Housini, I., Lang, C., and Schafer, A. J. Keratinocyte allografts accelerate healing of split-thickness donor sites: Applications for improved treatment of burns. Burn Care Rehabil. 14: 54, 1993.
19. Tagami, H., Ohi, M., Iwatsuki, K., Kanamaru, Y., Yamada, M., and Ichijo, B. Evaluation of the skin surface hydration in vivo by electrical measurement. J. Invest. Dermatol. 75: 500, 1980.
20. Stoner, M. L., and Wood, F. M. Cultured epithelial autograft "take" confirmed by the presence of cytokeratin 9. J. Invest. Dermatol. 112: 391, 1999.