Quantitative continuous assay for hyaluronan synthase

Analytical Biochemistry

Volumn 361, Issue 2, 2007, Pages 218-225

  Krupa, Joanne C ^a   Shaya, David ^b   Chi, Lianli ^c   Linhardt, Robert J ^c   Cygler, Miroslaw ^b,d   Withers, Stephen G ^e   Mort, John S ^a,b  

^a SHRINERS HOSPITAL FOR CHILDREN   (Canada)

^b MCGILL UNIVERSITY   (Canada)

^c RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^d NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^e UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Glycosaminoglycan; Hyaluronan synthase; Pasteurella multocida hyaluronan synthase; UDP glycosyltransferase

Indexed keywords

ELECTROPHORESIS; GLUCOSE; HYALURONIC ACID;

CONCENTRATION-DEPENDENT MANNERS; GLYCOSAMINOGLYCANS; GLYCOSYL TRANSFERASE; HYALURONAN; INHIBITION CONSTANTS; MECHANISTIC ASPECTS; PASTEURELLA MULTOCIDA; SYNTHASES;

ENZYME INHIBITION;

ENZYME; GLUCURONIC ACID; GLYCOSYLTRANSFERASE; HYALURONAN SYNTHASE; HYALURONIC ACID; N ACETYLGLUCOSAMINYLTRANSFERASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; TETRASACCHARIDE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE; URIDINE DIPHOSPHATE GLUCOSE;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME PURIFICATION; GEL ELECTROPHORESIS; NONHUMAN; OXIDATION; PASTEURELLA MULTOCIDA; PRIORITY JOURNAL; QUANTITATIVE ASSAY; TIME; ULTRAVIOLET SPECTROSCOPY;

PASTEURELLA MULTOCIDA;

EID: 33846399701     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.11.011     Document Type: Article

References (46)

1. Toyokawa K., Harayama H., and Miyake M. Exogenous hyaluronic acid enhances porcine parthenogenetic embryo development in vitro possibly mediated by CD44. Theriogenology 64 (2005) 378-392
2. Taylor K.R., and Gallo R.L. Glycosaminoglycans and their proteoglycans: host-associated molecular patterns for initiation and modulation of inflammation. FASEB J. 20 (2006) 9-22
3. Price R.D., Myers S., Leigh I.M., and Navsaria H.A. The role of hyaluronic acid in wound healing: assessment of clinical evidence. Am. J. Clin. Dermatol. 6 (2005) 393-402
4. Yabushita H., Kishida T., Fusano K., Kanyama K., Zhuo L., Itano N., Kimata K., and Noguchi M.Y. Role of hyaluronan and hyaluronan synthase in endometrial cancer. Oncol. Rep. 13 (2005) 1101-1105
5. Adamia S., Maxwell C.A., and Pilarski L.M. Hyaluronan and hyaluronan synthases: potential therapeutic targets in cancer. Curr. Drug Targets Cardiovasc. Haematol. Disord. 5 (2005) 3-14
6. Volpi N. Therapeutic applications of glycosaminoglycans. Curr. Med. Chem. 13 (2006) 1799-1810
7. Kelly M.A., Moskowitz R.W., and Lieberman J.R. Hyaluronan therapy: looking toward the future. Am. J. Orthop. 33 Suppl. 2 (2004) 23-28
8. Fujishiro M., Yahagi N., Nakamura M., Kakushima N., Kodashima S., Ono S., Kobayashi K., Hashimoto T., Yamamichi N., Tateishi A., Shimizu Y., Oka M., Ogura K., Kawabe T., Ichinose M., and Omata M. Successful outcomes of a novel endoscopic treatment for GI tumors: endoscopic submucosal dissection with a mixture of high-molecular-weight hyaluronic acid, glycerin, and sugar. Gastrointest. Endosc. 63 (2006) 243-249
9. Watt J.M., Swialto E., Wade M.M., and Champlin F.R. Regulation of capsule biosynthesis in serotype A strains of Pasteurella multocida. FEMS Microbiol. Lett. 225 (2003) 9-14
10. Chung J.Y., Wilkie I., Boyce J.D., Townsend K.M., Frost A.J., Ghoddusi M., and Adler B. Role of capsule in the pathogenesis of fowl cholera caused by Pasteurella multocida serogroup A. Infect. Immun. 69 (2001) 2487-2492
11. Wessels M.R., Moses A.E., Goldberg J.B., and DiCesare T.J. Hyaluronic acid capsule is a virulence factor for mucoid group A Streptococci. Proc. Natl. Acad. Sci. USA 88 (1991) 8317-8321
12. Jing W., and DeAngelis P.L. Dissection of the two transferase activities of the Pasteurella multocida hyaluronan synthase: two active sites exist in one polypeptide. Glycobiology 10 (2000) 883-889
13. Williams K.J., Halkes K.M., Kamerling J.P., and DeAngelis P.L. Critical elements of oligosaccharide acceptor substrates for the Pasteurella multocida hyaluronan synthase. J. Biol. Chem. 281 (2006) 5391-5397
14. Jing W., and DeAngelis P.L. Analysis of the two active sites of the hyaluronan synthase and the chondroitin synthase of Pasteurella multocida. Glycobiology 13 (2003) 661-671
15. DeAngelis P.L. Enzymological characterization of the Pasteurella multocida hyaluronic acid synthase. Biochemistry 35 (1996) 9768-9771
16. Itano N., Sawai T., Yoshida M., Lenas P., Yamada Y., Imagawa M., Shinomura T., Hamaguchi M., Yoshida Y., Ohnuki Y., Miyauchi S., Spicer A.P., McDonald J.A., and Kimata K. Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties. J. Biol. Chem. 274 (1999) 25085-25092
17. Yoshida M., Itano N., Yamada Y., and Kimata K. In vitro synthesis of hyaluronan by a single protein derived from mouse HAS1 gene and characterization of amino acid residues essential for the activity. J. Biol. Chem. 275 (2000) 497-506
18. Tlapak-Simmons V.L., Baggenstoss B.A., Clyne T., and Weigel P.H. Purification and lipid dependence of the recombinant hyaluronan synthases from Streptococcus pyogenes and Streptococcus equisimilis. J. Biol. Chem. 274 (1999) 4239-4245
19. Pummill P.E., and DeAngelis P.L. Alteration of polysaccharide size distribution of a vertebrate hyaluronan synthase by mutation. J. Biol. Chem. 278 (2003) 19808-19814
20. Tlapak-Simmons V.L., Baggenstoss B.A., Kumari K., and Heldermon C. Kinetic characterization of the recombinant hyaluronan synthases from Streptococcus pyogenes and Streptococcus equisimilis. J. Biol. Chem. 274 (1999) 4246-4253
21. Kumari K., Tlapak-Simmons V.L., Baggenstoss B.A., and Weigel P.H. The Streptococcal hyaluronan synthases are inhibited by sulfhydryl-modifying reagents, but conserved cysteine residues are not essential for enzyme function. J. Biol. Chem. 277 (2002) 13943-13951
22. Tlapak-Simmons V.L., Kempner E.S., Baggenstoss B.A., and Weigel P.H. The active Streptococcal hyaluronan synthases (HASs) contain a single HAS monomer and multiple cardiolipin molecules. J. Biol. Chem. 273 (1998) 26100-26109
23. DeAngelis P.L. Molecular directionality of polysaccharide polymerization by the Pasteurella multocida hyaluronan synthase. J. Biol. Chem. 274 (1999) 26557-26562
24. Stoolmiller A.C., and Dorfman A. The biosynthesis of hyaluronic acid by Streptococcus. J. Biol. Chem. 244 (1969) 236-246
25. Malinowski N.M., Cysyk R.L., and August E.M. A filter paper assay for hyaluronic acid synthetase: application to the enzyme from Swiss 3T3 fibroblasts. Biochem. Mol. Biol. Intl. 35 (1995) 1123-1132
26. Larjava H., Vaarala J., Saarni H., Penttinen R., and Hopsu-Favu V.K. A method for the hyaluronic acid synthetase assay in human skin. Arch. Dermatol. Res. 273 (1982) 199-204
27. Spicer A.P. In vitro assays for hyaluronan synthase. Methods Mol. Biol. 171 (2001) 373-382
28. Gosselin S., Alhussaini M., Streiff M.B., Takabayashi K., and Palcic M.M. A continuous spectrophotometric assay for glycosyltransferases. Anal. Biochem. 220 (1994) 92-97
29. Ly H.D., Lougheed B., Wakarchuk W.W., and Withers S.G. Mechanistic studies of a retaining α-galactosyltransferase from Neisseria meningitidis. Biochemistry 41 (2002) 5075-5085
30. Fitzgerald D.K., Colvin B., Mawal R., and Ebner K.E. Enzymic assay for galactosyl transferase activity of lactose synthetase and α-lactalbumin in purified and crude systems. Anal. Biochem. 36 (1970) 43-61
31. Brodbeck U., and Ebner K.E. Resolution of a soluble lactose synthetase into two protein components and solubilization of microsomal lactose synthetase. J. Biol. Chem. 24 (1966) 762-764
32. Davidson E.A. Specificity of pyruvate kinase. Biochim. Biophys. Acta 33 (1959) 238-240
33. Gill S.C., and von Hippel P.H. Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem. 182 (1989) 319-326
34. D'Auria G., Flores G., Falcigno L., Oliva R., Vacatello M., Corsaro M.M., Parrilli M., and Paolillo L. Hyaluronate tetrasaccharide-Cu(II) interaction: a NMR study. Biopolymers 70 (2003) 260-269
35. Reissig J.L., Strominger J.L., and Leloir L.F. A modified colorimetric method for the estimation of N-acetylamino sugars. J. Biol. Chem. 217 (1955) 959-966
36. Asteriou T., Deschrevel B., Delpech B., Bertrand P., Bultelle F., Merai C., and Vincent J.-C. An improved assay for the N-acetyl-d-glucosamine reducing ends of polysaccharides in the presence of proteins. Anal. Biochem. 293 (2001) 53-59
37. Starr C.M., Masada R.I., Hague C., Skop E., and Klock J.C. Fluorophore-assisted carbohydrate electrophoresis in the separation, analysis, and sequencing of carbohydrates. J. Chromatogr. A 720 (1996) 295-321
38. Calabro A., Midura R., Wang A., West L., Plaas A., and Hascall V.C. Fluorophore-assisted carbohydrate electrophoresis (FACE) of glycosaminoglycans. Osteoarthr. Cartil. 9 (2001) S16-S22
39. Gao N., and Lehrman M.A. Alternative sources of reagents and supplies for fluorophore-assisted carbohydrate electrophoresis (FACE). Glycobiology 13 (2003) 1G-3G
40. Jackson P. High-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides. Methods Enzymol. 230 (1994) 250-265
41. Weigel P.H. Functional characteristics and catalytic mechanisms of the bacterial hyaluronan synthases. IUBMB Life 54 (2002) 201-211
42. Tlapak-Simmons V.L., Baron C.A., Gotschall R., Haque D., Canfield W.M., and Weigel P.H. Hyaluronan biosynthesis by class I Streptococcal hyaluronan synthases occurs at the reducing end. J. Biol. Chem. 280 (2005) 13012-13018
43. Jedrzejas M.J. Structural and functional comparison of polysaccharide-degrading enzymes. Crit. Rev. Biochem. Mol. Biol. 35 (2000) 221-251
44. DeAngelis P.L., Oatman L.C., and Gay D.F. Rapid chemoenzymatic synthesis of monodisperse hyaluronan oligosaccharides with immobilized enzyme reactors. J. Biol. Chem. 278 (2003) 35199-35203
45. Pummill P.E., and DeAngelis P.L. Evaluation of critical structural elements of UDP-sugar substrates and certain cysteine residues of a vertebrate hyaluronan synthase. J. Biol. Chem. 277 (2002) 21610-21616
46. Tlapak-Simmons V.L., Baron C.A., and Weigel P.H. Characterization of the purified hyaluronan synthase from Streptococcus equisimilis. Biochemistry 43 (2004) 9234-9242