Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe

Tetrahedron Letters

Volumn 48, Issue 8, 2007, Pages 1341-1344

  Manabe, Yoshiyuki ^a   Sugimoto, Takanori ^a   Kawasaki, Tomoyuki ^a   Ueda, Minoru ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

Leaf opening substance; Nano gold; Nyctinasty; TEM; TEM probe

Indexed keywords

ANTIGEN; BENZOPHENONE; CELL RECEPTOR; FLUORESCEIN ISOTHIOCYANATE; GOLD;

ARTICLE; CASSIA MIMOSOIDES; CELL MEMBRANE; MECHANICAL PROBE; MOLECULAR WEIGHT; PHOTOAFFINITY LABELING; PLANT; PLANT CELL; PLANT LEAF; RATING SCALE; TRANSMISSION ELECTRON MICROSCOPY;

CHAMAECRISTA MIMOSOIDES;

EID: 33846356654     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tetlet.2006.12.128     Document Type: Article

References (10)

1. Darwin C. The Power of Movement in Plants. Third Thousand (1882), John Murray, London
2. Satter R.L., Gorton H.L., and Vogelmann T.C. The Pulvinus: Motor Organ for Leaf Movement (1990), American Society of Plant Physiologists
3. Ueda M., and Yamamura S. Angew. Chem., Int. Ed. 39 (2000) 1400-1414
4. Ueda M., and Nakamura Y. Nat. Prod. Rep. 23 (2006) 548-557
5. Hainfeld J.F., and Powell R.D. J. Histochem. Cytochem. 48 (2000) 471-480
6. Shigemori H., Sakai N., Miyoshi E., Shizuri Y., and Yamamura S. Tetrahedron 46 (1990) 383-394
7. Ueda M., Sawai Y., and Yamamura S. Tetrahedron Lett. 40 (1999) 3757-3760
8. Fujii T., Manabe Y., Sugimoto T., and Ueda M. Tetrahedron 61 (2005) 7874-7893
9. 19 -7.1 (c 0.50, MeOH).
10. 2 fragment (0.2 mg in 167 μL, Goat-Poly, Rockland) was diluted by 5 mM EDTA-0.1 M phosphate buffer (pH 6.0) to 1 mL, and then cysteamine hydrochloride (6.0 mg, Aldrich) was added to this solution. After being allowed to stand at rt for 1 h, the reaction mixture was separated by using gel filtration using Fast Desalting Column HR 10/10 (Amersham) with 0.02 M sodium phosphate (pH 6.5) containing 1 mM EDTA and 0.15 M NaCl, and then concentrated with Centricon YM-10 (Millipore). Aqueous solution of monomaleimido nano-gold (250 μL, Nanoprobe) was added to the resulting Fab′ fragment (0.1 mg), and then allowed to stand at 4 °C overnight. The solution was concentrated with Centricon YM-30 (Millipore), and then separated by Superdex 75 HR 10/30 with 0.02 M sodium phosphate (pH 7.4) containing 0.15 M NaCl to give nano-gold-bound anti-FITC Fab′ fragment.