Mitochondrial DNA assessment in adipocytes and peripheral blood mononuclear cells of HIV-infected patients with lipodystrophy according to a validated case definition

HIV Medicine

Volumn 8, Issue 1, 2007, Pages 32-37

  Casula, Miriam ^a,c   van der Valk, M ^b   Wit, F W ^a,b   Nievaard, M A ^b   Reiss, P ^a,b  

^a International Antiviral Therapy Evaluation Center   (Netherlands)

^b ACADEMIC MEDICAL CENTER   (Netherlands)

^c LATEC   (Netherlands)

Author keywords

HIV 1; Lipodystrophy; Mitochondrial toxicity; Nucleoside reverse transcriptase inhibitor; Stavudine

Indexed keywords

2',3' DIDEOXYNUCLEOSIDE DERIVATIVE; ANTIRETROVIRUS AGENT; DIDANOSINE; MITOCHONDRIAL DNA; PROTEINASE INHIBITOR; RNA DIRECTED DNA POLYMERASE INHIBITOR; STAVUDINE; ZALCITABINE;

ADIPOCYTE; ADULT; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE MARKER; DNA CONTENT; DNA DETERMINATION; DRUG EXPOSURE; FEMALE; HIV ASSOCIATED LIPODYSTROPHY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; HUMAN TISSUE; LUMBAR SPINE; MALE; MITOCHONDRIAL TOXICITY; PATHOGENESIS; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; STATISTICAL SIGNIFICANCE; SUBCUTANEOUS FAT; THIGH; TREATMENT DURATION;

ADIPOCYTES; ADIPOSE TISSUE; ADULT; DIDEOXYNUCLEOSIDES; DNA, MITOCHONDRIAL; FEMALE; HIV INFECTIONS; HIV-1; HIV-ASSOCIATED LIPODYSTROPHY SYNDROME; HUMANS; LUMBOSACRAL REGION; MALE; MIDDLE AGED; NETHERLANDS; REVERSE TRANSCRIPTASE INHIBITORS; STAVUDINE; THIGH;

EID: 33846323668     PISSN: 14642662     EISSN: 14681293     Source Type: Journal    
DOI: 10.1111/j.1468-1293.2007.00428.x     Document Type: Article

References (27)

1. Carr A, Cooper DA. Adverse effects of antiretroviral therapy. Lancet 2000; 356: 1423-1430.
2. Mallal SA, John M, Moore CB, James IR, McKinnon EJ. Contribution of nucleoside analogue reverse transcriptase inhibitors to subcutaneous fat wasting in patients with HIV infection. AIDS 2000; 14: 1309-1316.
3. Nolan D, Hammond E, James I, McKinnon E, Mallal S. Contribution of nucleoside-analogue reverse transcriptase inhibitor therapy to lipoatrophy from the population to the cellular level. Antiviral Ther 2003; 8: 617-626.
4. Saint-Marc T, Partisani M, Poizot-Martin I et al. A syndrome of peripheral fat wasting (lipodystrophy) in patients receiving long-term nucleoside analogue therapy. AIDS 1999; 13: 1659-1667.
5. Brinkman K, Smeitink JA, Romijn JA, Reiss P. Mitochondrial toxicity induced by nucleoside-analogue reverse-transcriptase inhibitors is a key factor in the pathogenesis of antiretroviral-therapy-related lipodystrophy. Lancet 1999; 354: 1112-1115.
6. Cossarizza A, Mussini C, Vigano A. Mitochondria in the pathogenesis of lipodystrophy induced by anti-HIV antiretroviral drugs: Actors or bystanders? Bioessays 2001; 23: 1070-1080.
7. Martin JL, Brown CE, Matthews-Davis N, Reardon JE. Effects of antiviral nucleoside analogs on human DNA polymerases and mitochondrial DNA synthesis. Antimicrob Agents Chemother 1994; 38: 2743-2749.
8. Zaera MG, Miro O, Pedrol E et al. Mitochondrial involvement in antiretroviral therapy-related lipodystrophy. AIDS 2001; 15: 1643-1651.
9. Shikuma CM, Hu N, Milne C et al. Mitochondrial DNA decrease in subcutaneous adipose tissue of HIV-infected individuals with peripheral lipoatrophy. AIDS 2001; 15: 1801-1809.
10. Walker UA, Brinkman K. NRTI induced mitochondrial toxicity as a mechanism for HAART related lipodystrophy: Fact or fiction? HIV Med 2001; 2: 163-165.
11. Chiappini F, Teicher E, Saffroy R et al. Prospective evaluation of blood concentration of mitochondrial DNA as a marker of toxicity in 157 consecutively recruited untreated or HAART-treated HIV-positive patients. Lab Invest 2004; 84: 908-914.
12. Hoy JF, Gahan ME, Carr A et al. Changes in mitochondrial DNA in peripheral blood mononuclear cells from HIV-infected patients with lipoatrophy randomized to receive abacavir. J Infect Dis 2004; 190: 688-692.
13. Nolan D, Hammond E, Martin A et al. Mitochondrial DNA depletion and morphologic changes in adipocytes associated with nucleoside reverse transcriptase inhibitor therapy. AIDS 2003; 17: 1329-1338.
14. Cossarizza A, Riva A, Pinti M et al. Increased mitochondrial DNA content in peripheral blood lymphocytes from HIV-infected patients with lipodystrophy. Antiviral Ther 2003; 8: 315-321.
15. Buffet M, Schwarzinger M, Amellal B et al. Mitochondrial DNA depletion in adipose tissue of HIV-infected patients with peripheral lipoatrophy. J Clin Virol 2005; 33: 60-64.
16. Miura T, Goto M, Hosoya N et al. Depletion of mitochondrial DNA in HIV-1-infected patients and its amelioration by antiretroviral therapy. J Med Virol 2003; 70: 497-505.
17. Walker UA, Bickel M, Lutke Volksbeck SI et al. Evidence of nucleoside analogue reverse transcriptase inhibitor associated genetic and structural defects of mitochondria in adipose tissue of HIV-infected patients. J Acquir Immune Defic Syndr 2002; 29: 117-121.
18. van der Valk M, Casula M, Weverling GJ et al. Prevalence of lipoatrophy and mitochondrial DNA content of blood and subcutaneous fat in HIV-1-infected patients randomly allocated to zidovudine- or stavudine-based therapy. Antiviral Ther 2004; 9: 385-393.
19. Carr A, Emery S, Law M, Puls R, Lundgren JD, Powderly WG. An objective case definition of lipodystrophy in HIV-infected adults: A case-control study. Lancet 2003; 361: 726-735.
20. Crisp M, Starkey KJ, Lane C, Ham J, Ludgate M. Adipogenesis in thyroid eye disease. Invest Ophthalmol Vis Sci 2000; 41: 3249-3255.
21. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990; 28: 495-503.
22. de Baar MP, Dobbelaer I, Timmermans EC et al. Declined mitochondrial DNA levels in peripheral blood mononuclear cells due to HIV infection can further decline or rise depending on the mitochondrial toxicity of the used antiviral drug combination. Program and Abstracts of the 5th International Workshop on Adverse Drug Reactions and Lipodystrophy in HIV, Paris. London, International Medical Press Ltd, 2003 [Abstract L37].
23. Banas B, Kost BP, Goebel FD. Platelets, a typical source of error in real-time PCR quantification of mitochondrial DNA content in human peripheral blood cells. Eur J Med Res 2004; 9: 371-377.
24. Timmermans EC, Tebas P, Ruiter JP, Wanders RJ, de Ronde A, de Baar MP. Real-time nucleic acid sequence-based amplification assay to quantify changes in mitochondrial DNA concentrations in cell cultures and blood cells from HIV-infected patients receiving antiviral therapy. Clin Chem 2006; 52: 979-987.
25. Miró O, Lopez S, Pedrol E et al. Mitochondrial DNA depletion and respiratory chain enzyme deficiencies are present in peripheral blood mononuclear cells of HIV-infected patients with HAART-related lipodystrophy. Antiviral Ther 2003; 8: 333-338.
26. Cherry CL, Gahan ME, McArthur JC, Lewin SR, Hoy JF, Wesselingh SL. Exposure to dideoxynucleosides is reflected in lowered mitochondrial DNA in subcutaneous fat. J Acquir Immune Defic Syndr 2002; 30: 271-277.
27. Mussini C, Pinti M, Bugarini R et al. Effect of treatment interruption monitored by CD4 cell count on mitochondrial DNA content in HIV-infected patients: A prospective study. AIDS 2005; 19: 1627-1633.