Role for the nonessential N terminus of FtsN in divisome assembly

Journal of Bacteriology

Volumn 189, Issue 2, 2007, Pages 646-649

  Goehring, Nathan W ^a   Robichon, Carine ^a   Beckwith, Jon ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; PEPTIDOGLYCAN; PROTEIN FTSK; PROTEIN FTSN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CELL DIVISION; ESCHERICHIA COLI; FTSA GENE; FTSI GENE; FTSK GENE; FTSQ GENE; GENE MUTATION; GENE OVEREXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION; TEMPERATURE SENSITIVE MUTANT;

BINDING SITES; BLOTTING, WESTERN; CELL CYCLE PROTEINS; CELL DIVISION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GREEN FLUORESCENT PROTEINS; MEMBRANE PROTEINS; MUTATION; PENICILLIN-BINDING PROTEINS; PEPTIDOGLYCAN GLYCOSYLTRANSFERASE; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP;

ESCHERICHIA COLI;

EID: 33846198296     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00992-06     Document Type: Article

References (16)

1. Buddelmeijer, N., and J. Beckwith. 2004. A complex of the Escherichia coli cell division proteins FtsL, FtsB and FtsQ forms independently of its localization to the septal region. Mol. Microbiol. 52:1315-1327.
2. Chen, J. C., and J. Beckwith. 2001. FtsQ, FtsL and FtsI require FtsK, but not FtsN, for co-localization with FtsZ during Escherichia coli cell division. Mol. Microbiol. 42:395-413.
3. Dai, K., Y. Xu, and J. Lutkenhaus. 1993. Cloning and characterization of ftsN, an essential cell division gene in Escherichia coli isolated as a multicopy suppressor of ftsA12(Ts). J. Bacteriol. 175:3790-3797.
4. Dai, K., Y. Xu, and J. Lutkenhaus. 1996. Topological characterization of the essential Escherichia coli cell division protein FtsN. J. Bacteriol. 178:1328-1334.
5. Di Lallo, G., M. Fagioli, D. Barionovi, P. Ghelardini, and L. Paolozzi. 2003. Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation. Microbiology 149:3353-3359.
6. Draper, G. C., N. McLennan, K. Begg, M. Masters, and W. D. Donachie. 1998. Only the N-terminal domain of FtsK functions in cell division. J. Bacteriol. 180:4621-4627.
7. Geissler, B., and W. Margolin. 2005. Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK. Mol. Microbiol. 58:596-612.
8. Goehring, N. W., M. D. Gonzalez, and J. Beckwith. 2006. Premature targeting of cell division proteins to midcell reveals hierarchies of protein interactions involved in divisome assembly. Mol. Microbiol. 61:33-45.
9. Goehring, N. W., F. Gueiros-Filho, and J. Beckwith. 2005. Premature targeting of a cell division protein to midcell allows dissection of divisome assembly in Escherichia coli. Genes Dev. 19:127-137.
10. Goehring, N. W., I. Petrovska, D. Boyd, and J. Beckwith. 2007. Mutants, suppressors, and wrinkled colonies: mutant alleles of the cell division gene ftsQ point to functional domains in FtsQ and a role for domain 1C of FtsA in divisome assembly. J. Bacteriol. 189:633-645.
11. Guzman, L. M., D. S. Weiss, and J. Beckwith. 1997. Domain-swapping analysis of FtsI, FtsL, and FtsQ, bitopic membrane proteins essential for cell division in Escherichia coli. J. Bacteriol. 179:5094-5103.
12. Karimova, G., N. Dautin, and D. Ladant. 2005. Interaction network among Escherichia coli membrane proteins involved in cell division as revealed by bacterial two-hybrid analysis. J. Bacteriol. 187:2233-2243.
13. Piette, A., C. Fraipont, T. Den Blaauwen, M. E. Aarsman, S. Pastoret, and M. Nguyen-Disteche. 2004. Structural determinants required to target penicillin-binding protein 3 to the septum of Escherichia coli. J. Bacteriol. 186:6110-6117.
14. Ursinus, A., F. van den Ent, S. Brechtel, M. de Pedro, J. V. Holtje, J. Lowe, and W. Vollmer. 2004. Murein (peptidoglycan) binding property of the essential cell division protein FtsN from Escherichia coli. J. Bacteriol. 186:6728-6737.
15. Weiss, D. S., J. C. Chen, J. M. Ghigo, D. Boyd, and J. Beckwith. 1999. Localization of FtsI (PBP3) to the septal ring requires its membrane anchor, the Z ring, FtsA, FtsQ, and FtsL. J. Bacteriol. 181:508-520.
16. Wissel, M. C., J. L. Wendt, C. J. Mitchell, and D. S. Weiss. 2005. The transmembrane helix of the Escherichia coli division protein FtsI localizes to the septal ring. J. Bacteriol. 187:320-328.