Proteolytic stability of insecticidal toxins expressed in recombinant bacilli

Applied and Environmental Microbiology

Volumn 73, Issue 1, 2007, Pages 218-225

  Yang, Yankun ^a,b   Wang, Liwei ^b   Gaviria, Adelaida ^b   Yuan, Zhiming ^a   Berry, Colin ^b  

^a WUHAN INSTITUTE OF VIROLOGY   (China)

^b CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEOLYTIC STABILITY; RECOMBINANT BACILLI;

BACTERIA; BIODEGRADATION; CRYSTALLINE MATERIALS; ENZYMES; GENES; INSECTICIDES;

TOXIC MATERIALS;

BACTERIAL ENZYME; BACTERIAL TOXIN; CRY1AA TOXIN; INSECTICIDE; MTX1 TOXIN; MUTANT PROTEIN; SERINE PROTEINASE; SPHERICASE; UNCLASSIFIED DRUG;

BACTERIUM; ENZYME ACTIVITY; GENE EXPRESSION; GENETICALLY MODIFIED ORGANISM; INSECTICIDE; MUTATION; PROTEIN; RECOMBINATION; SPORULATION; TOXIN;

ARTICLE; BACILLUS SPHAERICUS; BACILLUS THURINGIENSIS; BACTERIAL GENE; BACTERIAL SPORE; BACTERIAL STRAIN; BIN GENE; CRYSTAL; CULTURE MEDIUM; DEVELOPMENTAL STAGE; ENZYME ACTIVE SITE; ENZYME PURIFICATION; ENZYME SPECIFICITY; HOST; MUTATION; NONHUMAN; PROMOTER REGION; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN STABILITY; RECEPTOR BINDING; SPOROGENESIS;

AMINO ACID SEQUENCE; ANIMALS; BACILLUS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BASE SEQUENCE; CULEX; ENDOTOXINS; ESCHERICHIA COLI; HEMOLYSIN PROTEINS; MOLECULAR SEQUENCE DATA; PEPTIDE HYDROLASES; RECOMBINATION, GENETIC;

BACILLI (CLASS); BACILLUS SPHAERICUS; BACILLUS THURINGIENSIS;

EID: 33846138364     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.01100-06     Document Type: Article

References (34)

1. Abdul-Rauf, M., and D. J. Ellar. 1999. Mutations of loop 2 and loop 3 residues in domain II of Bacillus thuringiensis Cry1C delta-endotoxin affect insecticidal specificity and initial binding to Spodoptera littoralis and Aedes aegypti midgut membranes. Curr. Microbiol. 39:94-98.
2. Almog, O., A. Gonzalez, D. Klein, H. M. Greenblatt, S. Braun, and G. Shoham. 2003. The 0.93Å crystal structure of sphericase: a calcium-loaded serine protease from Bacillus sphaericus. J. Mol. Biol. 332:1071-1082.
3. Almond, B. D., and D. H. Dean. 1994. Intracellular proteolysis and limited diversity of the Bacillus thuringiensis Cry1A family of the insecticidal crystal proteins. Biochem. Biophys. Res. Commun. 201:788-794.
4. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
5. Arantes, O., and D. Lereclus. 1991. Construction of cloning vectors for Bacillus thuringiensis. Gene 108:115-119.
6. Arora, N., A. Selvapandiyan, N. Agrawal, and R. K. Bhatnagar. 2003. Relocation of vegetative insecticidal protein into mother cell of Bacillus thuringiensis. Biochem. Biophys. Res. Commun. 310:158-162.
7. Berry, C., J. Hindley, A. F. Ehrhardt, T. Grounds, I. de Souza, and E. W. Davidson. 1993. Genetic determinants of the host range of the Bacillus sphaericus mosquito larvicidal toxins. J. Bacteriol. 175:510-518.
8. Bourgouin, C., A. Delécluse, F. de la Torre, and J. Szulmajster. 1990. Transfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression. Appl. Environ. Microbiol. 56:340-344.
9. de Maagd, R. A., A. Bravo, C. Berry, N. Crickmore, and H. E. Schnepf. 2003. Structure, diversity, and evolution of protein toxins from spore-forming entomopathogenic bacteria. Annu. Rev. Genet. 37:409-433.
10. Finney, D. J. 1971. Probit analysis-a statistical treatment of the sigmoid response curve, 3rd ed. Cambridge University Press, Cambridge, United Kingdom.
11. Graf, R., and H. Briegel. 1985. Isolation of trypsin isozymes from the mosquito Aedes aegypti (L.). Insect Biochem. 15:611-618.
12. Kasman, L. M., A. A. Lukowiak, S. F. Garczynski, R. J. McNall, P. Youngman, and M. J. Adang. 1998. Phage display of a biologically active Bacillus thuringiensis toxin. Appl. Environ. Microbiol. 64:2995-3003.
13. Li, J. D., J. Carroll, and D. J. Ellar. 1991. Crystal structure of insecticidal delta-endotoxin from Bacillus thuringiensis at 2.5 Å resolution. Nature 353: 815-821.
14. Liu, J.-W., A. G. Porter, B. Y. Wee, and T. Thanabalu. 1996. New gene from nine Bacillus sphaericus strains encoding highly conserved 35.8-kilodalton mosquitocidal toxins. Appl. Environ. Microbiol. 62:2174-2176.
15. Liu, X. S., and D. H. Dean. 2006. Redesigning Bacillus thuringiensis Cry1Aa toxin into a mosquitocidal toxin. Protein Eng. Design Select. 19:107-111.
16. Marzari, R., P. Edomi, R. K. Bhatnagar, S. Ahmad, A. Selvapandiyan, and A. Bradbury. 1997. Phage display of Bacillus thuringiensis Cry1A(a) insecticidal toxin. FEBS Lett. 411:27-31.
17. Pacheco, S., I. Gomez, R. Sato, A. Bravo, and M. Soberon. 2006. Functional display of Bacillus thuringiensis Cry1Ac toxin on T7 phage. J. Invertebr. Pathol. 92:45-49.
18. Rajamohan, F., M. K. Lee, and D. H. Dean. 1998. Bacillus thuringiensis insecticidal proteins: molecular mode of action. Prog. Nucleic Acid Res. Mol. Biol. 60:1-27.
19. Reinert, D. J., I. Carpusca, K. Aktories, and G. E. Schulz. 2006. Structure of the mosquitocidal toxins from Bacillus sphaericus. J. Mol. Biol. 357:1226-1236.
20. Schirmer, J., I. Just, and K. Aktories. 2002. The ADP-ribosylating mosquitocidal toxin from Bacillus sphaericus. J. Biol. Chem. 277:11941-11948.
21. Siezen, R. J., and J. A. Leunissen. 1997. Subtilases: the superfamily of subtilisin-like serine proteases. Protein Sci. 6:501-523.
22. Silva-Filha, M.-H., L. Regis, C. Nielsen-LeRoux, and J.-F. Charles. 1995. Low-level resistance to Bacillus sphaericus in a field-treated population of Culex quinquefasciatus (Diptera: Culicidae). J. Econ. Entomol. 88:525-530.
23. Silva-Filha, M. H., C. Nielsen-LeRoux, and J.-F. Charles. 1997. Binding kinetics of Bacillus sphaericus binary toxin to midgut brush-border membranes of Anopheles and Culex sp. mosquito larvae. Eur. J. Biochem. 247: 754-761.
24. Smith, G. P., and D. J. Ellar. 1994. Mutagenesis of two surface-exposed loops of the Bacillus thuringiensis CryIC delta-endotoxin affects insecticidal specificity. Biochem. J. 302:611-616.
25. Tabashnik, B. E., Y. Carriere, T. J. Dennehy, S. Morin, M. S. Sisterson, R. T. Roush, A. M. Shelton, and J. Z. Zhao. 2003. Insect resistance to transgenic Bt crops: lessons from the laboratory and field. J. Econ. Entomol. 96:1031-1038.
26. Tabashnik, B. E., N. L. Cushing, N. Finson, and M. W. Johnson. 1990. Field development of resistance to Bacillus thuringiensis in diamondback moth (Lepidoptera: Plutellidae). J. Econ. Entomol. 83:1671-1676.
27. Thanabalu, T., J. Hindley, and C. Berry. 1992. Proteolytic processing of the mosquitocidal toxin from Bacillus sphaericus SSII-1. J. Bacteriol. 174:5051-5056.
28. Thanabalu, T., J. Hindley, J. Jackson-Yap, and C. Berry. 1991. Cloning, sequencing and expression of a gene encoding a 100-kilodalton mosquitocidal toxin from Bacillus sphaericus SSII-1. J. Bacteriol. 173:2776-2785.
29. Thanabalu, T., and A. G. Porter. 1996. A Bacillus sphaericus gene encoding a novel type of mosquitocidal toxin of 31.8kDa. Gene 170:85-89.
30. Thanabalu, T., and A. G. Porter. 1995. Efficient expression of a 100-kilodalton mosquitocidal toxin in protease-deficient recombinant Bacillus sphaericus. Appl. Environ. Microbiol. 61:4031-4036.
31. Vilchez, S., J. Jacoby, and D. J. Ellar. 2004. Display of biologically functional insecticidal toxin on the surface of lambda phage. Appl. Environ. Microbiol. 70:6587-6594.
32. Wati, M., T. Thanabalu, and A. Porter. 1997. Gene from tropical Bacillus sphaericus encoding a protease closely related to subtilising from Antarctic bacilli. Biochim. Biophys. Acta 1352:56-62.
33. Yuan, Z., C. Nielsen-LeRoux, N. Pasteur, A. Delécluse, J. F. Charles, and R. Frutos. 1999. Cloning and expression of the binary toxin genes of Bacillus sphaericus C3-41 in a crystal minus B. thuringiensis subsp. israelensis. Wei Sheng Wu Xue Bao 39:29-35.
34. Yuan, Z., Y. M. Zhang, Q. X. Cai, and E. Y. Liu. 2000. High-level field resistance to Bacillus sphaericus C3-41 in Culex quinquefasciatus from Southern China. Biocontrol Sci. Technol. 10:41-49.