Assessment of anaerobic benzene degradation potential using 16S rRNA gene-targeted real-time PCR

Environmental Microbiology

Volumn 9, Issue 1, 2007, Pages 72-80

  Da Silva, Marcio L B ^a   Alvarez, Pedro J J ^a  

^a Rice University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BENZENE; PRIMER DNA; RIBOSOME DNA; RNA 16S;

ANAEROBIC BACTERIUM; ARTICLE; BIOREMEDIATION; ELECTROPHORESIS; GENE DOSAGE; GENETICS; METABOLISM; METHODOLOGY; POLYMERASE CHAIN REACTION; RNA GENE; WATER POLLUTANT;

BACTERIA, ANAEROBIC; BENZENE; BIODEGRADATION, ENVIRONMENTAL; DNA PRIMERS; DNA, RIBOSOMAL; ELECTROPHORESIS; GENE DOSAGE; GENES, RRNA; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; WATER POLLUTANTS, CHEMICAL;

DESULFOBACTERIUM;

EID: 33846096841     PISSN: 14622912     EISSN: 14622920     Source Type: Journal    
DOI: 10.1111/j.1462-2920.2005.01116.x     Document Type: Article

References (39)

1. Adamson, D.T., and Parkin, G.F. (1999) Biotransformation of mixtures of chlorinated aliphatic hydrocarbons by an acetate-grown methanogenic enrichment culture. Water Res 33: 1482-1494.
2. Alvarez, P.J.J., and Illman, W. (2005) Bioremediation and Natural Attenuation of Groundwater Contaminants: Process Fundamentals and Mathematical Models. Hoboken, NJ: John Wiley & Sons, p. 608.
3. Alvarez, P.J.J., and Vogel, T.M. (1995) Degradation of BTEX and their aerobic metabolites by indigenous microorganisms under nitrate-reducing conditions. Water Sci Technol 31: 15-28.
4. Anderson, R.T., and Lovley, D.R. (2000) Anaerobic bioremediation of benzene under sulfate-reducing conditions in a petroleum-contaminated aquifer. Environ Sci Technol 34: 2261-2266.
5. Archer, D.B., and Powell, G.E. (1985) Dependence of the specific growth-rate of methanogenic mutualistic cocultures on the methanogen. Arch Microbiol 141: 133-137.
6. Beller, H.R., Kane, S.R., Legler, T.C., and Alvarez, P.J.J. (2002) A real-time polymerase chain reaction method for monitoring anaerobic, hydrocarbon-degrading bacteria based on a catabolic gene. Environ Sci Technol 36: 3977-3984.
7. Bratbak, G. (1985) Bacterial biovolume and biomass estimations. Appl Environ Microbiol 49: 1488-1493.
8. 2-utilizing methanogenic bacteria. Appl Environ Microbiol 33: 1162-1169.
9. Chakraborty, R., and Coates, J.D. (2005) Hydroxylation and carboxylation-two crucial steps of anaerobic benzene degradation by Dechloromonas strain RCB. Appl Environ Microbiol 71: 5427-5432.
10. Coates, J.D., Chakraborty, R., Lack, J.G., O'Connor, S.M., Cole, K.A., Bender, K.S., and Achenbach, L.A. (2001) Anaerobic benzene oxidation coupled to nitrate reduction in pure culture by two strains of Dechloromonas. Nature 411: 1039-1043.
11. Corseuil, H.X., Hunt, C.S., Dos Santos, R.C.F., and Alvarez, P.J.J. (1998) The influence of the gasoline oxygenate ethanol on aerobic and anaerobic BTX biodegradation. Water Res 32: 2065-2072.
12. Da Silva, M.L.B., and Alvarez, P.J.J. (2002) Effects of ethanol versus MTBE on benzene, toluene, ethylbenzene, and xylene natural attenuation in aquifer columns. J Environ Eng-ASCE 128: 862-867.
13. Da Silva, M.L.B., and Alvarez, P.J.J. (2004) Enhanced anaerobic biodegradation of benzene-toluene-ethylbenzene-xylene-ethanol mixtures in bioaugmented aquifer columns. Appl Environ Microbiol 70: 4720-4726.
14. Edwards, E.A., and Grbic'-Galic', D. (1992) Complete mineralization of benzene by aquifer microorganisms under strictly anaerobic conditions. Appl Environ Microbiol 58: 2663-2666.
15. Edwards, E.A., and Grbic'-Galic', D. (1994) Anaerobic degradation of toluene and o-xylene by a methanogenic consortium. Appl Environ Microbiol 60: 313-322.
16. Edwards, E.A., Wills, L.E., Reinhard, M., and Grbic'-Galic', D. (1992) Anaerobic degradation of toluene and xylene by aquifer microorganisms under sulfate-reducing conditions. Appl Environ Microbiol 58: 794-800.
17. Ferris, M.J., Muyzer, G., and Ward, D.M. (1996) Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community. Appl Environ Microbiol 62: 340-346.
18. Ficker, M., Krastel, K., Orlicky, S., and Edwards, E. (1999) Molecular characterization of a toluene-degrading methanogenic consortium. Appl Environ Microbiol 65: 5576-5585.
19. Grbic'-Galic', D., and Vogel, T.M. (1987) Transformation of toluene and benzene by mixed methanogenic cultures. Appl Environ Microbiol 53: 254-260.
20. Guyot, J.P. (1986) Role of formate in methanogenesis from xylan by Cellulomonas sp associated with methanogens and Desulfovibrio vulgaris - inhibition of the aceticlastic reaction. FEMS Microbiol Lett 34: 149-153.
21. Heider, J., Spormann, A.M., Beller, H.R., and Widdel, F. (1998) Anaerobic bacterial metabolism of hydrocarbons. FEMS Microbiol Rev 22: 459-473.
22. Jones, W.J., Guyot, J.P., and Wolfe, R.S. (1984) Methanogenesis from sucrose by defined immobilized consortia. Appl Environ Microbiol 47: 1-6.
23. Kazumi, J., Caldwell, M.E., Suflita, J.M., Lovley, D.R., and Young, L.Y. (1997) Anaerobic degradation of benzene in diverse anoxic environments. Environ Sci Technol 31: 813-818.
24. Lovley, D.R., Coates, J.D., Woodward, J.C., and Phillips, E.J.P. (1995) Benzene oxidation coupled to sulfate reduction. Appl Environ Microbiol 61: 953-958.
25. National Academy of Sciences (2000) Natural Attenuation for Groundwater Remediation. Washington, DC, USA: National Academy of Sciences.
26. Phelps, C.D., Kerkhof, L.J., and Young, L.Y. (1998) Molecular characterization of a sulfate-reducing consortium which mineralizes benzene. FEMS Microbiol Ecol 27: 269-279.
27. Porteous, L.A., Seidler, R.J., and Watrud, L.S. (1997) An improved method for purifying DNA from soil for polymerase chain reaction amplification and molecular ecology applications. Mol Ecol 6: 787-791.
28. Powers, S.E., Rice, D., Dooher, B., and Alvarez, P.J.J. (2001a) Will ethanol-blended gasoline affect groundwater quality? Using ethanol instead of MTBE as a gasoline oxygenate could be less harmful to the environment. Environ Sci Technol 35: 24A-30A.
29. Powers, S.E., Hunt, C.S., Heermann, S.E., Corseuil, H.X., Rice, D., and Alvarez, P.J.J. (2001b) The transport and fate of ethanol and BTEX in groundwater contaminated by gasohol. Crit Rev Environ Sci Technol 31: 79-123.
30. Rooney-Varga, J.N., Anderson, R.T., Fraga, J.L., Ringelberg, D., and Lovley, D.R. (1999) Microbial communities associated with anaerobic benzene degradation in a petroleum-contaminated aquifer. Appl Environ Microbiol 65: 3056-3063.
31. Ruiz-Aguilar, G.M.L., O'Reilly, K., and Alvarez, P.J.J. (2002) A comparison of benzene and toluene plume lengths for sites contaminated with regular vs. ethanol-amended gasoline. Ground Water Monit Remediat 23: 48-53.
32. Suzuki, M.T., Taylor, L.T., and DeLong, E.F. (2000) Quantitative analysis of small-subunit rRNA genes in mixed microbial populations via 5'-nuclease assays. Appl Environ Microbiol 66: 4605-4614.
33. Ulrich, A.C., and Edwards, E.A. (2003) Physiological and molecular characterization of anaerobic benzene-degrading mixed cultures. Environ Microbiol 5: 92-102.
34. Ulrich, A.C., Beller, H.R., and Edwards, E.A. (2005) Metabolites detected during biodegradation of C-13(6)-benzene in nitrate-reducing and methanogenic enrichment cultures. Environ Sci Technol 39: 6681-6691.
35. United States Environmental Agency Technical Support Project (2000) Natural Attenuation for Groundwater Remediation. Washington, DC, USA: United States Environmental Agency Technical Support Project, p. 79.
36. United States Environmental Protection Agency (1998a) Carcinogenic Effects of Benzene: An Update, Vol. 63: 107. Washington, DC, USA: United States Environmental Protection Agency, p. 30495.
37. United States Environmental Protection Agency (1998b) Guide for remediation by natural attenuation. E943-98.
38. United States Environmental Protection Agency (2005) Semi-annual Mid-Year Activity Report. Washington, DC, USA: United States Environmental Protection Agency, pp. 1-7.
39. Zhou, J.Z., Bruns, M.A., and Tiedje, J.M. (1996) DNA recovery from soils of diverse composition. Appl Environ Microbiol 62: 316-322.