An arginine-to-proline mutation in a domain with undefined functions within the helicase protein (Nsp13) is lethal to the coronavirus infectious bronchitis virus in cultured cells

Virology

Volumn 358, Issue 1, 2007, Pages 136-147

  Fang, Shouguo ^a   Chen, Bo ^b   Tay, Felicia P L ^a   Ng, Beng Sern ^a   Liu, Ding Xing ^a,b  

^a INSTITUTE OF MOLECULAR AND CELL BIOLOGY   (Singapore)

^b NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

Author keywords

Arginine to proline mutation; Coronavirus infectious bronchitis virus; Helicase; Infectious clone; Infectivity

Indexed keywords

AMINO ACID; ARGININE; GLUTAMIC ACID; GLUTAMINE; GLYCINE; HELICASE; LEUCINE; LYSINE; NUCLEOTIDE; PAPAIN; PROLINE; PROTEIN NSP13; PROTEIN NSP3; PROTEIN NSP5; PROTEINASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; AVIAN INFECTIOUS BRONCHITIS VIRUS; CELL CULTURE; CLONE; CONTROLLED STUDY; CORONAVIRUS; GENE MUTATION; GENETIC MANIPULATION; IN VITRO STUDY; LETHALITY; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PATHOGENESIS; POINT MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSCRIPTION; RNA VIRUS; VERO CELL; VIROGENESIS; VIRUS CELL INTERACTION; VIRUS GENOME; VIRUS INFECTIVITY; VIRUS MUTANT;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CERCOPITHECUS AETHIOPS; INFECTIOUS BRONCHITIS VIRUS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE HYDROLASES; PLAQUE ASSAY; POINT MUTATION; RNA HELICASES; RNA, VIRAL; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTION, GENETIC; VERO CELLS; VIRAL PROTEINS; VIRUS REPLICATION;

AVIAN INFECTIOUS BRONCHITIS VIRUS; CORONAVIRUS; RNA VIRUSES;

EID: 33846074516     PISSN: 00426822     EISSN: 10960341     Source Type: Journal    
DOI: 10.1016/j.virol.2006.08.020     Document Type: Article

References (36)

1. Almazan F., Gonzalez J.M., Penzes Z., Izeta A., Calvo E., Plana-Duran J., and Enjuanes L. Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome. Proc. Natl. Acad. Sci. U.S.A. 97 (2000) 5516-5521
2. Boursnell M.E.G., Brown T.D.K., Foulds I.J., Green P.F., Tomley F.M., and Binns M.M. Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J. Gen. Virol. 68 (1987) 57-77
3. Casais R., Thiel V., Siddell S.G., Cavanagh D., and Britton P. Reverse genetics system for the avian coronavirus infectious bronchitis virus. J. Virol. 75 (2001) 12359-12369
4. Casais R., Dove B., Cavanagh D., and Britton P. Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. J. Virol. 77 (2003) 9084-9089
5. Casais R., Davies M., Cavanagh D., and Britton P. Gene 5 of the avian coronavirus infectious bronchitis virus is not essential for replication. J. Virol. 79 (2005) 8065-8078
6. Coley S.E., Lavi E., Sawicki S.G., Fu L., Schelle B., Karl N., Siddell S.G., and Thiel V. Recombinant mouse hepatitis virus strain A59 from cloned, full-length cDNA replicates to high titers in vitro and is fully pathogenic in vitro. J. Virol. 79 (2005) 3097-3106
7. Fang S.G., Shen S., Tay F.P., and Liu D.X. Selection of and recombination between minor variants lead to the adaptation of an avian coronavirus to primate cells. Biochem. Biophys. Res. Commun. 336 (2005) 417-423
8. Hodgson T., Britton P., and Cavanagh D. Neither the RNA nor the proteins of open reading frames 3a and 3b of the coronavirus infectious bronchitis virus are essential for replication. J. Virol. 80 (2006) 296-305
9. Ivanov K.A., Thiel V., Dobbe J.C., van der Meer Y., Snijder E.J., and Ziebuhr J. Multiple enzymatic activities associated with severe acute respiratory syndrome coronavirus helicase. J. Virol. 78 (2004) 5619-5632
10. Koetzner C.A., Parker M.M., Ricard C.S., Sturman L.S., and Masters P.S. Repair and mutagenesis of the genome of a deletion mutant of the coronavirus mouse hepatitis virus by targeted RNA recombination. J. Virol. 66 (1992) 1841-1848
11. Lim K.P., and Liu D.X. Characterization of a papain-like proteinase domain encoded by ORF1a of the coronavirus IBV and determination of the C-terminal cleavage site of an 87 kDa protein. Adv. Exp. Med. Biol. 440 (1998) 173-184
12. Lim K.P., and Liu D.X. Characterization of the two overlapping papain-like proteinase domains encoded in gene 1 of the coronavirus infectious bronchitis virus and determination of the C-terminal cleavage site of an 87 kDa protein. Virology 245 (1998) 303-312
13. Lim K.P., Ng L.F., and Liu D.X. Identification of a novel cleavage activity of the first papain-like proteinase domain encoded by open reading frame 1a of the coronavirus avian infectious bronchitis virus and characterization of the cleavage products. J. Virol. 74 (2000) 1674-1685
14. Liu D.X., Tibbles K.W., Cavanagh D., Brown T.D.K., and Brierley I. Identification, expression and processing of an 87K polypeptide encoded by ORF1a of the coronavirus infectious bronchitis virus. Virology 208 (1995) 47-54
15. Liu D.X., Xu H.Y., and Brown T.D.K. Proteolytic processing of the coronavirus infectious bronchitis virus 1a polyprotein: identification of a 10 kDa polypeptide and determination of its cleavage sites. J. Virol. 71 (1997) 1814-1820
16. Liu D.X., Shen S., Xu H.Y., and Wang S.F. Proteolytic mapping of the coronavirus infectious bronchitis virus 1b polyprotein: evidence for the presence of four cleavage sites of the 3C-like proteinase and identification of two novel cleavage products. Virology 246 (1998) 288-297
17. Marra M.A., Jones S.J.M., Astell C.R., Holt R.A., Brooks-Wilson A., Butterfield Y.S.N., Khattra J., Asano J.K., et al. The genome sequence of the SARS-associated coronavirus. Science 300 (2003) 1399-1404
18. Masters P.S., and Rottier P.J. Coronavirus reverse genetics by targeted RNA recombination. Curr. Top. Microbiol. Immunol. 287 (2005) 133-159
19. Ng L.F., and Liu D.X. Identification of a 24 kDa polypeptide processed from the coronavirus infectious bronchitis virus 1a polyprotein by the 3C-like proteinase and determination of its cleavage sites. Virology 243 (1998) 388-395
20. Ng L.F., and Liu D.X. Further characterization of the coronavirus infectious bronchitis virus 3C-like proteinase and determination of a new cleavage. Virology 272 (2000) 27-39
21. Ng L.F., and Liu D.X. Membrane association and dimerization of a cysteine-rich, 16-kDa polypeptide released from the C-terminal region of the coronavirus infectious bronchitis virus 1a polyprotein. J. Virol. 76 (2002) 6257-6267
22. Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R., Icenogle J.P., Penaranda S., et al. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300 (2003) 1394-1999
23. Sanchez C.M., Izeta A., Sanchez-Morgado J.M., Alonso S., Sola I., Balasch M., Plana-Duran J., and Enjuanes L. Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence. J. Virol. 73 (1999) 7607-7618
24. Seybert A., Posthuma C.C., van Dinten L.C., Snijder E.J., Gorbalenya A.E., and Ziebuhr J. A complex zinc finger controls the enzymatic activities of nidovirus helicases. J. Virol. 79 (2005) 696-704
25. Shen S., Wen Z.L., and Liu D.X. Emergence of an avian coronavirus infectious bronchitis virus (IBV) mutant with a truncated 3b gene: functional characterization of the 3b gene in pathogenesis and replication. Virology 311 (2003) 16-27
26. Shen S., Law Y.C., and Liu D.X. A Single amino acid mutation in the spike protein of coronavirus infectious bronchitis virus hampers its maturation and incorporation into virions at the nonpermissive temperature. Virology 326 (2004) 288-298
27. Thiel V., Herold J., Schelle B., and Siddell S.G. Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus. J. Gen. Virol. 82 (2001) 1273-1281
28. van Dinten L.C., den Boon J.A., Wassenaar A.L., Spaan W.J., and Snijder E.J. An infectious arterivirus cDNA clone: identification of a replicase point mutation that abolishes discontinuous mRNA transcription. Proc. Natl. Acad. Sci. U.S.A. 94 (1997) 991-996
29. van Dinten L.C., van Tol H., Gorbalenya A.E., and Snijder E.J. The predicted metal-binding region of the arterivirus helicase protein is involved in subgenomic mRNA synthesis, genome replication, and virion biogenesis. J. Virol. 74 (2000) 5213-5223
30. Xu H.Y., Lim K.P., Shen S., and Liu D.X. Further identification and characterization of novel intermediate and mature cleavage products released from the ORF 1b region of the avian coronavirus infectious bronchitis virus 1a/1b polyprotein. Virology 288 (2001) 212-222
31. Youn S., Collisson E.W., and Machamer C.E. Contribution of trafficking signals in the cytoplasmic tail of the infectious bronchitis virus spike protein to virus infection. J. Virol. 79 (2005) 13209-13217
32. Youn S., Leibowitz J.L., and Collisson E.W. In vitro assembled, recombinant infectious bronchitis viruses demonstrate that the 5a open reading frame is not essential for replication. Virology 332 (2005) 206-215
33. Yount B., Curtis K.M., and Baric R.S. Strategy for systematic assembly of large RNA and DNA genomes: transmissible gastroenteritis virus model. J. Virol. 74 (2000) 10600-10611
34. Yount B., Denison M.R., Weiss S.R., and Baric R.S. Systematic assembly of a full-length infectious cDNA of mouse hepatitis virus strain A59. J. Virol. 76 (2002) 11065-11078
35. Yount B., Curtis K.M., Fritz E.A., Hensley L.E., Jahrling P.B., Prentice E., Denison M.R., Geisbert T.W., and Baric R.S. Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus. Proc. Natl. Acad. Sci. U.S.A. 100 (2003) 12995-13000
36. Yount B., Roberts R.S., Sims A.C., Deming D., Frieman M.B., Sparks J., Denison M.R., Davis N., and Baric R.S. Severe acute respiratory syndrome coronavirus group-specific open reading frames encode nonessential functions for replication in cell cultures and mice. J. Virol. 79 (2005) 14909-14922