High-performance liquid chromatography method for rapid assessment of viral particle number in crude adenoviral lysates of mixed serotype

Gene Therapy

Volumn 14, Issue 2, 2007, Pages 180-184

  Kuhn, I ^a   Larsen, B ^a   Gross, C ^a   Hermiston, T ^a  

^a BAYER AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

SEPHAROSE;

ACCURACY; ADENOVIRUS VECTOR; ARTICLE; CELL LYSATE; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SEROTYPE; VIRAL GENE THERAPY; VIRUS CULTURE; VIRUS IDENTIFICATION; VIRUS PARTICLE;

ADENOVIRIDAE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GENE THERAPY; SEROTYPING; VIRION;

ADENOVIRIDAE;

EID: 33846013953     PISSN: 09697128     EISSN: 14765462     Source Type: Journal    
DOI: 10.1038/sj.gt.3302851     Document Type: Article

References (6)

1. Shabram PW, Giroux DD, Goudreau AM, Gregory RJ, Horn MT, Huyghe BG et al. Analytical anion-exchange HPLC of recombinant type-5 adenoviral particles. Hum Gene Ther 1997; 8: 453-465.
2. Blanche F, Cameron B, Barbot A, Ferrero L, Guillemin T, Guyot S et al. An improved anion-exchange HPLC method for the detection and purification of adenoviral particles. Gene Therapy 2000; 7: 1055-1062.
3. Tollefson AE, Hermiston T,Wold WSM. Preparation and Titration of Cesium Chloride Banded Adenovirus Stock. Humana Press: Totowa, NJ, 1999.
4. Lehmberg E, Traina JA, Chakel JA, Chang RJ, Parkman M, McCaman MT et al. Reversed-phase high-performance liquid chromatographic assay for the adenovirus type 5 proteome. J Chromatogr B 1999; 732: 411-423.
5. Maizel Jr JV, White DO, Scharff MD. The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion. Virology 1968; 36: 126-136.
6. Maizel Jr JV, White DO, Scharff MD. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology 1968; 36: 115-125