Rapid measurement of deuterium-labeled long-chain fatty acids in plasma by HPLC-ESI-MS

Journal of Lipid Research

Volumn 48, Issue 1, 2007, Pages 252-259

  Gagné, Sébastien ^a   Crane, Sheldon ^a   Huang, Zheng ^a   Chun, Sing Li ^a   Bateman, Kevin P ^a   Lévesque, Jean François ^a  

^a MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

Author keywords

Deuterium labeled oleic acid; Deuterium labeled palmitic acid; Deuterium labeled stearic acid; Electrospray ionization negative mode; High performance liquid chromatography electrospray ionization mass spectrometry; Quantification of plasma levels

Indexed keywords

DEUTERIUM; LONG CHAIN FATTY ACID;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; FATTY ACID ANALYSIS; FATTY ACID DESATURATION; FATTY ACID METABOLISM; FATTY ACID OXIDATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; INCIDENCE; LIPID BLOOD LEVEL; MALE; NONHUMAN; PRIORITY JOURNAL; RAT;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DEUTERIUM; FATTY ACIDS, NONESTERIFIED; HUMANS; ISOTOPE LABELING; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 33846008711     PISSN: 00222275     EISSN: 15397262     Source Type: Journal    
DOI: 10.1194/jlr.D600037-JLR200     Document Type: Article

References (26)

1. Bergen, W. G., and H. J. Mersmann. 2005. Comparative aspects of lipid metabolism: impact on contemporary research and use of animal models. J. Nutr. 135: 2499-2502.
2. Center for Disease Control and Prevention-U.S. Department of Health and Human Services, National Center for Health Statistics. Accessed May, 2006 at www.cdc.gov/nchs/fastats/overwt.htm.
3. Seppanen-Laakso, T., I. Laakso, and L. R. Hiltunen. 2002. Analysis of fatty acids by gas chromatography, and its relevance to research on health and nutrition. Anal. Chim. Acta. 465: 39-62.
4. Pawlosky, R. J., H. W. Sprecher, and N. Salem, Jr. 1992. High sensitivity negative ion GC-MS method for detection of desaturated and chain-elongated products of deuterated linoleic and linolenic acids. J. Lipid Res. 33: 1711-1717.
5. Pawlosky, R., A. Barnes, and N. Salem, Jr. 1994. Essential fatty acid metabolism in the feline: relationship between liver and brain production of long-chain polyunsaturated fatty acids. J. Lipid Res. 35: 2032-2040.
6. Lin, Y. H., R. J. Pawlosky, and N. Salem, Jr. 2005. Simultaneous quantitative determination of deuterium- and carbon-13 labeled essential fatty acids in rat plasma. J. Lipid Res. 46: 1974-1982.
7. Hilkert, A. W., C. B. Douthitt, H. J. Schluter, and W. A. Brand. 1999. Isotope ratio monitoring gas chromatography/mass spectrometry of D/H by high temperature conversion isotope ratio mass spectrometry. Rapid Commun. Mass Spectrom. 13: 1226-1230.
8. Brenna, J. T. 1997. Use of stable isotopes to study fatty acid and lipoprotein metabolism in man. Prostaglandins Leukot. Essent. Fatty Acids. 57: 467-472.
9. Morrison, W. R., and L. M. Smith. 1964. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. J. Lipid Res. 5: 600-608.
10. Juaneda, P. 2002. Utilisation of reversed-phase high-performance liquid chromatography as an alternative to silver-ion chromatography for the separation of cis- and trans-C18:1 fatty acid isomers. J. Chromatogr. A. 954: 285-289.
11. Gutnikov, G. 1995. Fatty acid profiles of lipid samples. J. Chromatogr. B Biomed. Appl. 671: 71-89.
12. Singh, G., A. Gutierrez, K. Xu, and I. A. Blair. 2000. Liquid chromatography/electron capture atmospheric pressure chemical ionization/mass spectrometry: analysis of pentafluorobenzyl derivatives of biomolecules and drugs in the attomole range. Anal. Chem. 72: 3007-3013.
13. Privett, O. S., and W. L. Erdahl. 1978. Practical aspects of liquid chromatography-mass spectrometry (LC-MS) of lipids. Chem. Phys. Lipids. 21: 361-387.
14. Oliw, E. H., C. Su, T. Skogstrom, and G. Benthin. 1998. Analysis of novel hydroperoxides and other metabolites of oleic, linoleic, and linolenic acids by liquid chromatography-mass spectrometry with ion trap MSn. Lipids. 33: 843-852.
15. Johnson, D. W. 2000. Alkyldimethylaminoethyl ester iodides for improved analysis of fatty acids by electrospray ionization tandem mass spectrometry. Rapid Commun. Mass Spectrom. 14: 2019-2024.
16. Johnson, D. W., M-U. Trinh, and T. Oe. 2003. Measurement of plasma pristanic, phytanic and very long chain fatty acids by liquid chromatography-electrospray tandem mass spectrometry for the diagnosis of peroxisomal disorders. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 798: 159-162.
17. Tsukamoto, Y., T. Santa, H. Saimaru, K. Imai, and T. Funatsu. 2005. Synthesis of benzofurazan derivatization reagents for carboxylic acids and its application to analysis of fatty acids in rat plasma by high-performance liquid chromatography-electrospray ionization mass spectrometry. Biomed. Chromatogr. 19: 802-808.
18. Rigol, A., A. Latorre, S. Lacorte, and D. Barcelo. 2003. Direct determination of resin and fatty acids in process waters of paper industries by liquid chromatography/mass spectrometry. J. Mass Spectrom. 38: 417-426.
19. Perret, D., A. Gentili, S. Marchese, M. Sergi, and L. Caporossi. 2004. Determination of free fatty acids in chocolate by liquid chromatography with tandem mass spectrometry. Rapid Commun. Mass Spectrom. 18: 1989-1994.
20. Nagy, K., A. Jakab, J. Fekete, and K. Vekey. 2004. An HPLC-MS approach for analysis of very long chain fatty acids and other apolar compounds on octadecyl-silica phase using partly miscible solvents. Anal. Chem. 76: 1935-1941.
21. Oudhoff, K. A., T. Sangster, E. Thomas, and I. D. Wilson. 2006. Application of microbore HPLC in combination with tandem MS for the quantification of rosuvastatin in human plasma. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 832: 191-196.
22. Yu, H., E. Lopez, S. W. Young, J. Luo, H. Tian, and P. Cao. 2006. Quantitative analysis of free fatty acids in rat plasma using matrixassisted laser desorption/ionization time-of-flight mass spectrometry with meso-tetrakis porphyrin as matrix. Anal. Biochem. 354: 182-191.
23. Rock, D. A., B. N. Perkins, J. Wahlstrom, and J. P. Jones. 2003. A method for determining two substrates binding in the same active site of cytochrome P450BM3: an explanation of high energy omega product formation. Arch. Biochem. Biophys. 416: 9-16.
24. Berg, J. M., J. L. Tymoczko, L. Stryer, and N. D. Clarke. 2002. Fatty acid metabolism. In Biochemistry. 5th edition. W. H. Freeman and Company, New York. Chapter 22.
25. Lands, W. E. 1995. Long-term fat intake and biomarkers. Am. J. Clin. Nutr. 61 (Suppl. 3): 721-725.
26. Kimura, T., H. Fukuda, and N. Iritani. 2005. Labeled acetate incorporation into lipids and lipid elimination after oral administration in rat liver and adipose tissue. J. Nutr. Sci. Vitaminol. (Tokyo). 51: 104-109.