A hexanucleotide selected for increased cellular uptake in cis contains a highly active CpG-motif in human B cells and primary peripheral blood mononuclear cells

Immunology

Volumn 120, Issue 2, 2007, Pages 261-272

  Mende, Miriam ^a,b   Hopert, Anne ^a   Wünsche, Winfried ^a   Overhoff, Marita ^a   Detzer, Anke ^a   Börngen, Kirsten ^a   Schlenke, Peter ^a   Kirchner, Holger ^a   Sczakiel, Georg ^a,b  

^a UNIVERSITY HOSPITAL SCHLESWIG HOLSTEIN   (Germany)

^b Kompetenzzentrum fuer Drug Design und Target Monitoring   (Germany)

Author keywords

B cells; Cellular uptake; CpG; Oligonucleotides

Indexed keywords

CYTOSINE; GUANOSINE; OLIGONUCLEOTIDE; PHOSPHATE; PHOSPHOROTHIOIC ACID DERIVATIVE;

ARTICLE; B LYMPHOCYTE; BLOOD CELL; CELL ACTIVITY; CELL STIMULATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOSTIMULATION; MAMMAL CELL; MONONUCLEAR CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

B-LYMPHOCYTES; BASE SEQUENCE; CELLS, CULTURED; COMBINATORIAL CHEMISTRY TECHNIQUES; CPG ISLANDS; DNA MUTATIONAL ANALYSIS; DOSE-RESPONSE RELATIONSHIP, IMMUNOLOGIC; GENE LIBRARY; HUMANS; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE ACTIVATION; MOLECULAR SEQUENCE DATA; OLIGODEOXYRIBONUCLEOTIDES; TUMOR CELLS, CULTURED;

EID: 33845992884     PISSN: 00192805     EISSN: 13652567     Source Type: Journal    
DOI: 10.1111/j.1365-2567.2006.02497.x     Document Type: Article

References (31)

1. Krieg AM. CpG motifs: the active ingredient in bacterial extracts? Nat Med 2003 2004; 9:831-5.
2. Krieg AM. CpG motifs in bacterial DNA and their immune effects. Annu Rev Immunol 2002; 20:709-60.
3. Dalpke A, Zimmermann S, Heeg K. CpG-oligonucleotides in vaccination: signaling and mechanisms of action. Immunobiology 2001; 204:667-76.
4. Klinman DM. Immunotherapeutic uses of CpG oligodeoxynucleotides. Nat Rev Immunol 2004; 4:249-58.
5. Mutwiri GK, Nichani AK, Babiuk S, Babiuk LA. Strategies for enhancing the immunostimulatory effects of CpG oligodeoxynucleotides. J Control Release 2004; 97:1-17.
6. Bartz H, Mendoza Y, Gebker M, Fischborn T, Heeg K, Dalpke A. Poly-guanosine strings improve cellular uptake and stimulatory activity of phosphodiester CpG oligonucleotides in human leukocytes. Vaccine 2004; 23:148-55.
7. Leifer CA, Kennedy MN, Mazzoni A, Lee C, Kruhlak MJ, Segal DM. TLR9 is localized in the endoplasmic reticulum prior to stimulation. J Immunol 2004; 173:1179-83.
8. Latz E, Schoenemeyer A, Visintin A, et al. TLR9 signals after translocating from the ER to CpG DNA in the lysosome. Nat Immunol 2004; 5:190-8.
9. Zhao Q, Song X, Waldschmidt T, Fisher E, Krieg AM. Oligonucleotide uptake in human hematopoietic cells is increased in leukemia and is related to cellular activation. Blood 1996; 88:1788-95.
10. Laktionov PP, Dazard JE, Vives E, Rykova EY, Piette J, Vlassov VV, Lebleu B. Characterisation of membrane oligonucleotide-binding proteins and oligonucleotide uptake in keratinocytes. Nucl Acids Res 1999; 27:2315-24.
11. Gray GD, Basu S, Wickstrom E. Transformed and immortalized cellular uptake of oligodeoxynucleoside phosphorothioates, 30-alkylamino oligodeoxynucleotides, 2′-O-methyl oligoribonucleotides, oligodeoxynucleoside methylphosphonates, and peptide nucleic acids. Biochem Pharmacol 1997; 53:1465-76.
12. Budker V, Budker T, Zhang G, Subbotin V, Loomis A, Wolff JA. Hypothesis: naked plasmid DNA is taken up by cells in vivo by a receptor-mediated process. J Gene Med 2000; 2:76-88.
13. Doerfler W, Hohlweg U, Muller K, Remus R, Heller H, Hertz J. Foreign DNA integration - perturbations of the genome - oncogenesis. Ann N Y Acad Sci 2001; 945:276-88.
14. Lehmann MJ, Sczakiel G. Spontaneous uptake of biologically active recombinant DNA by mammalian cells via a selected DNA segment. Gene Ther 2005; 12:446-51.
15. Etore F, Tenu JP, Teiger E, Adnot S, Lonchampt MO, Pirotzki E, Le Doan T. Sequence dependency of the internalization and distribution of phosphorothioate oligonucleotides in vascular smooth muscle cells. Biochem Pharmacol 1998; 55:1465-73.
16. Tari AM, Lopez-Berestein G. Cellular uptake of antisense oligonucleotides. Curr Opin Invest Drugs 2001; 2:1450-3.
17. de Diesbach P, Berens C, N'Kuli F, Monsigny M, Sonveaux E, Wattiez R, Courtoy PJ. Identification, purification and partial characterisation of an oligonucleotide receptor in membranes of HepG2 cells. Nucl Acids Res 2000; 28:868-74.
18. de Diesbach P, N'Kuli F, Delmee M, Courtoy PJ. Infection by Mycoplasma hyorhinis strongly enhances uptake of antisense oligonucleotides. a reassessment of receptor-mediated endocytosis in the HepG2 cell line. Nucl Acids Res 2003; 31:886-92.
19. Schubbert R, Lettmann C, Doerfler W. Ingested foreign (phage M13) DNA survives transiently in the gastrointestinal tract and enters the bloodstream of mice. Mol Generalgenet 1994; 242:495-504.
20. Schubbert R, Renz D, Schmitz B, Doerfler W. Foreign (M13) DNA ingested by mice reaches peripheral leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalently linked to mouse DNA. Proc Natl Acad Sci USA 1997; 94:961-6.
21. Menezes J, Leibold W, Klein G, Clements G. Establishment and characterization of an Epstein-Barr virus (EBV)-negative lymphoblastoid B cell line (BJA-B) from an exceptional, EBV-genome-negative African Burkitt's lymphoma. Biomedicine 1975; 22:276-84.
22. Overhoff M, Wunsche W, Sczakiel G. Quantitative detection of siRNA and single-stranded oligonucleotides: relationship between uptake and biological activity of siRNA. Nucleic Acids Res 2004; 32:e170.
23. Schroer J, Holker I, Doerfler W. Adenovirus type 12 DNA firmly associates with mammalian chromosomes early after virus infection or after DNA transfer by the addition of DNA to the cell culture medium. J Virol 1997; 71:7923-32.
24. Hartmann G, Krieg AM. Mechanism and function of a newly identified CpG DNA motif in human primary B cells. J Immunol 2000; 164:944-53.
25. Jahrsdorfer B, Jox R, Muhlenhoff L, et al. Modulation of malignant B cell activation and apoptosis by bcl-2 antisense ODN and immunostimulatory CpG ODN. J Leukoc Biol 2002; 72:83-92.
26. Hartmann G, Weeratna RD, Ballas ZK, et al. Delineation of a CpG phosphorothioate oligodeoxynucleotide for activating primate immune responses in vitro and in vivo. J Immunol 2000; 164:1617-24.
27. Hartmann G, Krug A, Waller-Fontaine K, Endres S. Oligodeoxynucleotides enhance lipopolysaccharide-stimulated synthesis of tumor necrosis factor: dependence on phosphorothioate modification and reversal by heparin. Mol Med 1996; 2:429-38.
28. Wu CC, Castro JE, Motta M, Cottam HB, Kyburz D, Kipps TJ, Corr M, Carson DA. Selection of oligonucleotide aptamers with enhanced uptake and activation of human leukemia B cells. Hum Gene Ther 2003; 14:849-60.
29. Sester DP, Naik S, Beasley SJ, Hume DA, Stacey KJ. Phosphorothioate backbone modification modulates macrophage activation by CpG DNA. J Immunol 2000; 165:4165-73.
30. Dalpke AH, Zimmermann S, Albrecht I, Heeg K. Phosphodiester CpG oligonucleotides as adjuvants. polyguanosine runs enhance cellular uptake and improve immunostimulative activity of phosphodiester CpG oligonucleotides in vitro and in vivo. Immunology 2002; 106:102-12.
31. Lee SW, Song MK, Baek KH, et al. Effects of a hexameric deoxyriboguanosine run conjugation into CpG oligodeoxynucleotides on their immunostimulatory potentials. J Immunol 2000; 165:3631-9.