High-frequency embryogenesis, regeneration of broccoli (Brassica oleracea var. italica) and analysis of genetic stability by RAPD

Scientia Horticulturae

Volumn 111, Issue 3, 2007, Pages 203-208

  Qin, Ying ^a   Li, Hong Ling ^b   Guo, Yang Dong ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b Agricultural Technology Extension Station in Beijing   (China)

Author keywords

Broccoli; Explant; Genetic stability; RAPD marker; Regeneration; Somatic Embryogenesis

Indexed keywords

DICOTYLEDON; DNA; EMBRYONIC DEVELOPMENT; GENETIC ANALYSIS; GENETIC MARKER; GENOTYPE; GROWTH REGULATOR; POLYMERASE CHAIN REACTION; REGENERATION;

BRASSICA OLERACEA VAR. CAPITATA; BRASSICA OLERACEA VAR. ITALICA;

EID: 33845989120     PISSN: 03044238     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.scienta.2006.10.022     Document Type: Article

References (31)

1. 3 and genotype. Plant Cell Rep. 24 (2005) 649-654
2. Astarini I.A., Plummer J.A., Lancaster R.A., and Yan G.J. Fingerprinting of cauliflower cultivars using RAPD markers. Aust. J. Agric. Res. 55 (2004) 117-124
3. Bhalla P.L., and Smith N. Comparison of shoot regeneration potential from seedling explants of Australian cauliflowers (Brassica oleracea var. botrytis) varieties. Aust. J. Agric. Res. 49 (1998) 1261-1266
4. Cao J., and Earle E.D. Transgene expression in broccoli (Brassica oleracea var. italica) clones propagated in vitro via leaf explants. Plant Cell Rep. 21 (2003) 789-796
5. Chen L.P., Zhang M.F., Li C.S., and Hirata Y. Production of interspecific somatic hybrids between tuber mustard (Brassica juncea) and red cabbage (Brassica oleracea). Plant Cell Tissue Organ Cult. 80 (2005) 305-311
6. Christey M.C., and Earle E.D. Regeneration of Brassica oleracea from peduncle explants. Horticult. Sci. 26 (1991) 1069-1072
7. Crockett P.A., Bhalla P.L., Lee C.K., and Singh M.B. RAPD analysis of seed purity in a commercial hybrid cabbage (Brassica oleracea var. capitata) cultivar. Genome 43 (2000) 317-321
8. Crockett P.A., Singh M.B., Lee C.K., and Bhalla P.L. Genetic purity analysis of hybrid broccoli (Brassica oleracea var. italica) seeds using RAPD PCR. Aust. J. Agric. Res. 53 (2002) 51-54
9. Dale P., Al-Kaff N., and Bavage A. Transgene expression and stability in Brassica. Acta Horticult. 459 (1998) 167-171
10. Divaret I., and Thomas G. Use of RAPD markers to analyze the genetic variability of a collection of Brassica oleracea L. Acta Horticult. 459 (1998) 255-262
11. Guo Y.D., Sewón P., and Pulli S. Improved embryogenesis from anther culture and plant regeneration in timothy. Plant Cell Tissue Organ Cult. 57 (1999) 85-93
12. Guo Y.D., and Pulli S. Isolated microspore culture and plant regeneration in rye (Secale cereale L.). Plant Cell Rep. 19 (2000) 875-880
13. Guo Y.D., Yli-Mattila T., and Pulli S. Analysis of timothy (Phleum pratense L.) genetic variation with RAPD and UP-PCR. Hereditas 138 (2003) 101-113
14. Guo Y.D., Mizukami Y., and Yamada T. Genetic characterization of androgenic progeny derived from Lolium perenne x Festuca pratensis cultivars. New Phytol. 166 (2005) 455-464
15. Hansen G., and Wright M.S. Recent advances in the transformation of plants. Trends Plant Sci. 4 (1999) 226-231
16. Hu J., and Quiros C.F. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep. 10 (1991) 501-505
17. Khehra G.S., and Mathias R.J. The interaction of genotype, explant and media on the regeneration of shoots from complexes explants of Brassica napus L. J. Exp. Bot. 43 (1992) 115-120
18. King G.J. Morphological development in Brassica oleracea is modulated by in vivo treatment with 5-azacytidine. J. Horticult. Sci. 70 (1995) 333-342
19. Lazaro A., and Aguinagalde I. Genetic diversity in Brassica oleracea L. (Cruciferae) and wild relatives (2n = 18) using isozymes. Ann. Bot. 82 (1998) 821-828
20. Leroy X.J., Leon K., Charles G., and Branchard M. Cauliflower somatic embryogenesis and analysis of regenerant stability by ISSRs. Plant Cell Rep. 19 (2000) 1102-1107
21. Leroy X.J., Leon K., Hily J.M., Chaumeil P., and Branchard M. Detection of in vitro culture-induced instability through inter-simple sequence repeat analysis. Theor. Appl. Genet. 102 (2001) 885-891
22. Lim H.T., You Y.S., Park E.J., Song Y.N., and Park H.K. High plant regeneration, genetic stability of regenerants, and genetic transformation of herbicide resistance gene (bar) in Chinese cabbage (Brassica campestris ssp. pekinensis). Acta Horticult. 459 (1998) 199-208
23. Metz T.D., Dixit R., and Earle E.D. Agrobacterium tumefaciens-mediated transformation of broccoli (Brassica oleracea var. italica) and cabbage (B. oleracea var. capitata). Plant Cell Rep. 15 (1995) 287-292
24. Munthali M.T., Newbury H.J., and Ford-Lloyd B.V. The detection of somaclonal variants of beet using RAPD. Plant Cell Rep. 15 (1996) 474-478
25. Puddephat I.J., Robinson H.T., Fenning T.M., Barbara D.J., Morton A., and Pink D.A.C. Recovery of phenotypically normal transgenic plants of Brassica oleracea upon Agrobacterium rhizogenes-mediated co-transformation and selection of transformed hairy roots by GUS assay. Mol. Breed. 7 (2001) 229-242
26. Ozeki Y., Davies E., and Takeda J. Somatic variation during long term subculturing of plant cells caused by insertion of a transposable element in a phenylalanine ammonia-lyase (PAL) gene. Mol. Gen. Genet. 28 (1997) 407-416
27. Sparrow P.A.C., Dale P.J., and Irwin J.A. The use of phenotypic markers to identify Brassica oleracea genotypes for routine high-throughput Agrobacterium-mediated transformation. Plant Cell Rep. 23 (2004) 64-70
28. Stipic M., and Campion B. An improved protocol for androgenesis in cauliflowers (Brassica oleracea var. botrytis). Plant Breed. 116 (1997) 153-157
29. Wrage K., and Writer S. A new era for value-added vegetables? Bringing anti-cancer compounds to the produce shelves. Seed Crop Ind. 45 (1994) 4-15
30. Zhang F.L., Aoki S., and Takahata Y. RAPD markers linked to microspore embryogenic ability in Brassica crops. Euphytica 131 (2003) 207-213
31. Zhang Y., and Bhalla P.L. In vitro shoot regeneration from commercial cultivars of Australian canola (Brassica napus L.). Aust. J. Agric. Res. 55 (2004) 753-756