Secretion of biologically active human epidermal growth factor from Escherichia coli using Yersinia pestis Caf1 signal peptide

Journal of Microbiology, Immunology and Infection

Volumn 39, Issue 5, 2006, Pages 366-371

  Liu, Yi Lin ^a   Huang, Liang Min ^b   Lin, Wen Po ^b   Tsai, Chung Chin ^b   Lin, Tsung Shun ^b   Hu, Yu Hua ^b   Chen, Hquan Shin ^b   Han, Jun Ming ^b   Wang, Hsian Jenn ^b   Liu, Yu Tien ^b  

^a TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

^b NATIONAL DEFENSE MEDICAL CENTER   (Taiwan)

Author keywords

Bacterial proteins; Caf1; Protein sorting signals; Recombinant fusion proteins; Yersinia pestis

Indexed keywords

BACTERIAL PROTEIN; DNA FRAGMENT; EPIDERMAL GROWTH FACTOR; HYBRID PROTEIN; PROTEIN CAF1; RECOMBINANT EPIDERMAL GROWTH FACTOR; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; GENETIC TRANSFECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN FUNCTION; PROTEIN SECRETION; PROTEIN SYNTHESIS; YERSINIA PESTIS;

BACTERIAL PROTEINS; CELL WALL; EPIDERMAL GROWTH FACTOR; ESCHERICHIA COLI; HUMANS; MOLECULAR CHAPERONES; PERIPLASM; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

EID: 33845979462     PISSN: 16841182     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. Gregory H. Isolation and structure of urogastrone and its relationship to epidermal growth factor. Nature 1975;257:325-7.
2. Nakagawa S, Yoshida S, Mirao Y, Kasua S, Fuwa T. Biological effects of biosynthetic human EGF on the growth of mammalian cells in vitro. Differentiation 1985;29:294-8.
3. Clare JJ, Romanos MA, Rayment FB, Rowedder JE, Smith MA, Payne MM, et al. Production of mouse epidermal growth factor in yeast: high-level secretion using Pichia pastoris strains containing multiple gene copies. Gene 1991;105:205-12.
4. Jeong KJ, Lee SY. Secretory production of human leptin in Escherichia coli. Biotechnol Bioeng 2000;67:398-407.
5. Galyov EE, Smirnov OYu, Karlishev AV, Volkovoy KI, Denesyuk AI, Nazimov IV, et al. Nucleotide sequence of the Yersinia pestis gene encoding F1 antigen and the primary structure of the protein. Putative T and B cell epitopes. FEBS Lett 1990;277:230-2.
6. Galyov EE, Karlishev AV, Chernovskaya TV, Dolgikh DA, Smirnov OYu, Volkovoy KI, et al. Expression of the envelope antigen F1 of Yersinia pestis is mediated by the product of caf1M gene having homology with the chaperone protein PapD of Escherichia coli. FEBS Lett 1991;286:79-82.
7. von Heijne G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 1986;14:4683-90.
8. Zavialov AV, Batchikova NV, Korpela T, Petrovskaya LE, Korobko VG, Kersley J, et al. Secretion of recombinant protein via the chaperone/usher pathway in Escherichia coli. Appl Environ Microbiol 2001;67:1805-14.
9. Kim E, Fu J, Wilson DB, Shuler ML. Expression of human epidermal growth factor by Escherichia coli in continuous culture. Biotechnol Lett 1992;14:339-44.
10. Yadwad VB, Wilson S, Ward OP. Production of human epidermal growth factor by an ampicillin-resistant recombinant Escherichia coli strain. Biotechnol Lett 1994;16:885-90.
11. Clements JM, Catlin GH, Prince MJ, Edwards RM. Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences. Gene 1991;106:267-71.
12. Ebisu S, Takagi H, Kadowaki K, Yamagata H, Udaka S. Production of human epidermal growth factor by Bacillus brevis increased with use of a stable plasmid from B. brevis 481. Biosci Biotechnol Biochem 1992;56:812-3.
13. Engler DA, Matsunami RK, Campion SR, Stringer CD, Stevens A, Niyogi SK. Cloning of authentic human epidermal growth factor as a bacterial secretory protein and its initial structure-function analysis by site-directed mutagenesis. J Biol Chem 1988;263:12384-90.
14. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989.
15. Neu HC, Heppel LA. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem 1965;240:3685-92.
16. Schagger H, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Chem 1987;166:368-79.
17. Derynck R, Roberts AB, Winkler ME, Chen EY, Goeddel DV. Human transforming growth factor-alpha: precursor structure and expression in E. coli. Cell 1984;38:287-97.
18. Savage CR Jr, Hash JH, Cohen S. Epidermal growth factor. Location of disulfide bonds. J Biol Chem 1973;248:7669-72.