Endothelial cell density in donor corneas: A comparison of automatic software programs with manual counting

Cornea

Volumn 26, Issue 1, 2007, Pages 80-83

  Hirneiss, Christoph ^a   Schumann, Ricarda G ^a   Grüterich, Martin ^a   Welge Luessen, Ulrich C ^a   Kampik, Anselm ^a   Neubauer, Aljoscha S ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Automated endothelium counting; Corneal endothelium; Endothelial cell density; Organ culture

Indexed keywords

ARTICLE; AUTOANALYSIS; AUTOANALYZER; CELL COUNT; CELL DENSITY; COMPUTER ANALYSIS; COMPUTER PROGRAM; CONTROLLED STUDY; CORNEA DONOR; CORNEA ENDOTHELIUM; DIGITAL COMPUTER; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MEDICAL SPECIALIST; ORGAN CULTURE; ORGAN DONOR; PHOTOGRAPHY; PRIORITY JOURNAL; VALIDATION PROCESS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CELL COUNT; ENDOTHELIUM, CORNEAL; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; MIDDLE AGED; OPHTHALMOLOGY; ORGAN CULTURE TECHNIQUES; PHOTOGRAPHY; SOFTWARE; TISSUE DONORS;

EID: 33845966398     PISSN: 02773740     EISSN: None     Source Type: Journal    
DOI: 10.1097/ICO.0b013e31802be629     Document Type: Article

References (18)

1. Maas-Reijs J, Pels E, Tullo AB. Eye banking in Europe 1991-1995. Acta Ophthalmol Scand. 1997;75:541-543.
2. Nishimura JK, Hodge DO, Bourne WM. Initial endothelial cell density and chronic endothelial cell loss rate in corneal transplants with late endothelial failure. Ophthalmology. 1999;106:1962-1965.
3. Bourne WM. Cellular changes in transplanted human corneas. Cornea. 2001;20:560-569.
4. Armitage WJ, Dick AD, Bourne WM. Predicting endothelial cell loss and long-term corneal graft survival. Invest Ophthalmol Vis Sci. 2003;44:3326-3331.
5. Thuret G, Chiquet C, Bernal F, et al. Prospective, randomized clinical and endothelial evaluation of 2 storage times for cornea donor tissue in organ culture at 31 degrees C. Arch Ophthalmol. 2003;121:442-450.
6. Thuret G, Manissolle C, Acquart S, et al. Is manual counting of corneal endothelial cell density in eye banks still acceptable? The French experience. Br J Ophthalmol. 2003;87:1481-1486.
7. Lester JM, McFarland JL, Bursell SE, et al. Automated morphometric analysis of corneal endothelial cells. Invest Ophthalmol Vis Sci. 1981;20:407-410.
8. Delbosc B, Cantenot L, Fellmann D, et al. Morphométrie semi-automatisée in vitro de l'endothélium cornéen humain conservé. J Fr Ophtalmol. 1986;9:445-454.
9. Gain P, Thuret G, Kodjikian L, et al. Automated tri-image analysis of stored corneal endothelium. Br J Ophthalmol. 2002;86:801-808.
10. Bohnke M. A new system for storing donor corneas. Klin Monatsbl Augenheilkd. 1991;198:135-137.
11. Amann J, Holley GP, Lee SB, et al. Increased endothelial cell density in the paracentral and peripheral regions of the human cornea. Am J Ophthalmol. 2003;135:584-590.
12. Kohlhof JK, Ruprecht KW. Manual endothelial cell enumeration for transplanted corneas in comparison to software controlled, automatic enumeration. Klin Monatsbl Augenheilkd. 2004;221:473-478.
13. Altman DG, Bland JM. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986;7:307-310.
14. Barisani-Asenbauer T, Baumgartner I, Grabner G, et al. Automated digital image analysis of organ culture preserved donor corneas. Ophthalmic Res. 1993;25:94-99.
15. Seitz B, Muller EE, Langenbucher A, et al. Reproducibility and validity of a new automatic method of specular microscopy analysis of corneal endothelium. Ophthalmologe. 1997;94:127-135.
16. Reinhard T, Spelsberg H, Holzwarth D, et al. Endothelial evaluation of corneal transplants by digital imaging. Klin Monatsbl Augenheilkd. 1999;214:407-411.
17. Gavet Y, Meulle A, Deb N, et al. Endothelial cell count and morphometry by the tri image analyser sambacornee: multicentric validation. 16th Annual Meeting of the European Eye Bank Association, Barcelona, Spain, January, 18-19, 2004.
18. Thuret G, Manissolle C, Acquart S, et al. Urgent need for normalization of corneal graft quality controls in French eye banks. Transplantation. 2004;78:1299-1302.